식물공장에서 양액의 종류 및 PPFD가 배초향의 생장 및 항산화 물질에 미치는 영향 Response of Nutrient Solution and Photosynthetic Photon Flux Density for Growth and Accumulation of Antioxidant in Agastache rugosa under Hydroponic Culture Systems원문보기
배초향은 항동맥경화나 항박테리아의 특성을 가지는 한약재에 널리 사용되는 영년생 약용식물이다. 연구의 목적은 수경재배에서 배양액의 종류와 PPFD값에 따른 배초향의 생장 및 항산화 물질의 변화를 조사하는 것이다. 배초향은 주야간 16:8 시간의 일장조건에서 150과 $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD 조건과 일본원시(HES), 서울시립대(UOS), 유럽채소연구소(EVR), 오오츠카 배양액(OTS)을 이용하여 6주간 재배하였다. OTS 배양액조건에서 자란 배초향의 지상부 및 지하부 건물중은 다른 배양액 처리구와 비교하여 유의적으로 높았다. 배초향의 틸리아닌 함량은 OTS 처리에서 가장 높았으며 다음으로 EVR, HES, UOS 순서로 낮아졌다. 총 아카세틴의 함량은 EVR처리에서 가장 높았으나 OTS처리와는 유의적 차이를 보이지 않았다. 또한 $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD 조건에서 자란 배초향은 PPFD 150처리구와 비교하여 유의적으로 생체중과 건물중이 증가하였으며 기능성 물질은 틸리아닌과 아카세틴의 함량도 높았다. 본 연구는 수경재배 방식을 이용하여 식물공장에서 배초향을 재배할 경우 $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD 조건과 OTS 배양액 조건에서 경제적인 광원조건으로 최적 바이오매스 생산량과 틸라아닌과 아카세틴의 함량을 증가시킬 수 있을 것으로 제안한다.
배초향은 항동맥경화나 항박테리아의 특성을 가지는 한약재에 널리 사용되는 영년생 약용식물이다. 연구의 목적은 수경재배에서 배양액의 종류와 PPFD값에 따른 배초향의 생장 및 항산화 물질의 변화를 조사하는 것이다. 배초향은 주야간 16:8 시간의 일장조건에서 150과 $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD 조건과 일본원시(HES), 서울시립대(UOS), 유럽채소연구소(EVR), 오오츠카 배양액(OTS)을 이용하여 6주간 재배하였다. OTS 배양액조건에서 자란 배초향의 지상부 및 지하부 건물중은 다른 배양액 처리구와 비교하여 유의적으로 높았다. 배초향의 틸리아닌 함량은 OTS 처리에서 가장 높았으며 다음으로 EVR, HES, UOS 순서로 낮아졌다. 총 아카세틴의 함량은 EVR처리에서 가장 높았으나 OTS처리와는 유의적 차이를 보이지 않았다. 또한 $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD 조건에서 자란 배초향은 PPFD 150처리구와 비교하여 유의적으로 생체중과 건물중이 증가하였으며 기능성 물질은 틸리아닌과 아카세틴의 함량도 높았다. 본 연구는 수경재배 방식을 이용하여 식물공장에서 배초향을 재배할 경우 $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD 조건과 OTS 배양액 조건에서 경제적인 광원조건으로 최적 바이오매스 생산량과 틸라아닌과 아카세틴의 함량을 증가시킬 수 있을 것으로 제안한다.
Agastache rugosa, is a perennial medicinal plant commonly used in Chinese herbalism, and may have anti-atherogenic and antibacterial properties. Here in this study, we investigated the growth and variations in antioxidant contents of A. rugosa in response to nutrient solution and photosynthetic phot...
Agastache rugosa, is a perennial medicinal plant commonly used in Chinese herbalism, and may have anti-atherogenic and antibacterial properties. Here in this study, we investigated the growth and variations in antioxidant contents of A. rugosa in response to nutrient solution and photosynthetic photon flux density (PPFD) with artificial lighting for a hydroponics culture. Fluorescent light at 150, and $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD with a 16/8 (light/dark) photoperiod, combined with four different nutrient solutions [developed by Horticulture experiment station in Japan (HES), University of Seoul (UOS), Europe vegetable research center (EVR), Otsuka-house 1A (OTS)], were used in a hydroponics culture system for 6 weeks. The shoot and root dry weights of A. rugosa grown with the OTS were significantly higher than those of other nutrient solutions. The amount of tilianin was the highest grown with the OTS, followed by EVR, HES, and UOS. Total acacetin content was the highest in A. rugosa grown under EVR which was statistically similar with OTS. The A. rugosa grown under $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD produced higher fresh weight and both acacetin and tilianin contents than that grown under $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD. The present results suggested that OTS along with $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD could be an optimum growing condition for better growth and higher accumulation of tilianin and acacetin contents in A. rugosa with hydroponic culture systems in a plant factory.
Agastache rugosa, is a perennial medicinal plant commonly used in Chinese herbalism, and may have anti-atherogenic and antibacterial properties. Here in this study, we investigated the growth and variations in antioxidant contents of A. rugosa in response to nutrient solution and photosynthetic photon flux density (PPFD) with artificial lighting for a hydroponics culture. Fluorescent light at 150, and $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD with a 16/8 (light/dark) photoperiod, combined with four different nutrient solutions [developed by Horticulture experiment station in Japan (HES), University of Seoul (UOS), Europe vegetable research center (EVR), Otsuka-house 1A (OTS)], were used in a hydroponics culture system for 6 weeks. The shoot and root dry weights of A. rugosa grown with the OTS were significantly higher than those of other nutrient solutions. The amount of tilianin was the highest grown with the OTS, followed by EVR, HES, and UOS. Total acacetin content was the highest in A. rugosa grown under EVR which was statistically similar with OTS. The A. rugosa grown under $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD produced higher fresh weight and both acacetin and tilianin contents than that grown under $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD. The present results suggested that OTS along with $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD could be an optimum growing condition for better growth and higher accumulation of tilianin and acacetin contents in A. rugosa with hydroponic culture systems in a plant factory.
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제안 방법
At 4 weeks after transplanting, seedlings of A. rugosa grown under different nutrient solution, were analyzed for detection of tilianin and acacetin in the hydroponics culture system. Both tilianin and acacetinin content varied significantly in response to different nutrient solution (Fig.
Each experiment was conducted twice with completely randomized designs. All data were subjected to ANOVA followed by Duncan’s multiple rate test at P ≤ 0.
Four nutrient solutions, developed by Horticultural Experiment Station in Japan (HES), The University of Seoul (UOS), European Vegetable R&D Center (EVR), and Otsuka-house 1A (OTS) were used as experimental treatment in this study (Table 1).
대상 데이터
5 me L-1 PO4-P, 1 me L-1 Mg, and 4 me L-1 K with micro nutrient solutions) during seedling growth stage. Fifty two seedlings at 42 days aged (at four-leaf stage) were selected for four nutrient solution treatments. Thirteen seedlings for one treatment were planted maintaining 120 mm planting distance and cultured at 22±1℃ (day) and 18±1℃ (night) in a culture container (460 mm long × 330 mm wide × 110 mm high) for 4 weeks.
데이터처리
All data were subjected to ANOVA followed by Duncan’s multiple rate test at P ≤ 0.05, 0.01 (JMP, SAS Institute Inc., Cary, NC, USA).
성능/효과
rugosa grown under different nutrient solution. For acacetin accumulation, EVR showed the best performance which was statistically similar grown under OTS whereas the lowest acacetin was accumulated in A. rugosa grown under UOS. Sorgoleone content in the root of sorghum greatly influenced by the different concentration of Hoagland solution (Uddin et al.
In particular, by five weeks after transplanting, the total acacetin content under the PPFD of the 200μmol m–2 s–1 condition increased by approximately 49% compared to the PPFD 150 condition and the total tilianin content under the same condition increased by approximately 40% by six weeks after transplanting (Fig. 4).
Perillaldehyde concentrations, standard functional materials in green perilla (Perilla frutescens), was significantly different between combinations of the highest PPFD (among 100, 200, and 300μmol m–2 s–1) with the highest EC (among 1.0, 2.0, and 3.0 dS m-1), and the lowest PPFD with the lowest EC.
후속연구
rugosa were also increased in the light intensity of 200μmol m–2 s–1 which is applied for plant growth. Additional experiments with increased PPFD for plant growth and accumulation of bioactive compounds of A. rugosa are required.
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