Although there has been a steady increase in the prevalence of food allergies worldwide in recent decades, no effective therapeutic strategies have been developed. Modulation of the gut microbiota composition and/or function through probiotics has been highlighted as a promising target for protectio...
Although there has been a steady increase in the prevalence of food allergies worldwide in recent decades, no effective therapeutic strategies have been developed. Modulation of the gut microbiota composition and/or function through probiotics has been highlighted as a promising target for protection against food allergies. In this study, we aimed to investigate the allergy-reducing effects of a probiotic mixture (P5: Lactococcus lactis KF140, Pediococcus pentosaceus KF159, Lactobacillus pentosus KF340, Lactobacillus paracasei 698, and Bacillus amyloliquefaciens 26N) in mice with ovalbumin (OVA)-induced food allergy. Administration of P5 significantly suppressed the oral OVA challenge-induced anaphylactic response and rectal temperature decline, and reduced diarrhea symptoms. Moreover, P5 also significantly inhibited the secretion of IgE, Th2 cytokines (interleukin (IL)-4, IL-5, IL-10, and IL-13), and Th17 cytokines (IL-17), which were increased in mice with OVA-induced food allergy, and induced generation of CD4+Foxp3+ regulatory T cells. These results revealed that P5 may have applications as a preventive agent against food allergy.
Although there has been a steady increase in the prevalence of food allergies worldwide in recent decades, no effective therapeutic strategies have been developed. Modulation of the gut microbiota composition and/or function through probiotics has been highlighted as a promising target for protection against food allergies. In this study, we aimed to investigate the allergy-reducing effects of a probiotic mixture (P5: Lactococcus lactis KF140, Pediococcus pentosaceus KF159, Lactobacillus pentosus KF340, Lactobacillus paracasei 698, and Bacillus amyloliquefaciens 26N) in mice with ovalbumin (OVA)-induced food allergy. Administration of P5 significantly suppressed the oral OVA challenge-induced anaphylactic response and rectal temperature decline, and reduced diarrhea symptoms. Moreover, P5 also significantly inhibited the secretion of IgE, Th2 cytokines (interleukin (IL)-4, IL-5, IL-10, and IL-13), and Th17 cytokines (IL-17), which were increased in mice with OVA-induced food allergy, and induced generation of CD4+Foxp3+ regulatory T cells. These results revealed that P5 may have applications as a preventive agent against food allergy.
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제안 방법
In a preliminary study searching for anti-allergic probiotics, we found five probiotics (Lactobacillus pentosus KF 340, Lactococcus lactis KF140, Bacillus amyloliquefaciens 26N, Lactobacillus paracasei 698, and Pediococcus pentosaceus KF159 isolated from Korea traditional foods) that possess the IL-4 suppressive effect and Treg inducing effect via in vitro test. Therefore, in this study, we aimed to investigate the effects of a mixture of these five probiotics on alleviation of allergic symptoms, including diarrhea and anaphylactic response, in an OVA-induced food allergy model.
To evaluate the modulatory effects of P5 on the Th1/Th2 balance, we examined the expression patterns of IFN-γ and IL-4 in mLN-derived lymphocytes (isolated from OVA sensitized mice) co-cultured with P5 in the presence of OVA.
To investigate the preventive effects of P5, lyophilized P5 was suspended in saline and orally administered (5 × 108 CFU/head) every day from days 28 to 40 (Fig. 1).
To investigation the effects of oral administration of P5 on total IgE and specific IgE levels in an OVA-induced food allergy model, sera were obtained from each group after the fifth OVA challenge. Total IgE and OVA-specific IgE were dramatically increased in the sham group.
대상 데이터
Twenty-eight BALB/c mice (5 weeks old, female, 18–20 g) were purchased from Orient Bio Inc. (Korea).
데이터처리
Data were analyzed using ANOVA followed by Duncan’s multiple-range test.
The significance of differences between experimental conditions was analyzed using one-way analysis of variance (ANOVA) followed by Duncan’s multiple-range test.
이론/모형
For analysis of cytokines (IL-10 and IL-12), the cells were adjusted to 5 × 106 cells/ml in RPMI medium using the trypan blue exclusion method.
For cytokine analysis, the cells were adjusted to 5 ×106 cells/ml in RPMI medium using the trypan blue exclusion method.
Subsequently, the cells were adjusted to 5 × 106 cells/ml in RPMI medium using the trypan blue exclusion method.
성능/효과
To confirm that the inhibitory effects of P5 on the Th2 and Th17 immune responses were caused by Treg induction, P5 and mLNs were co-cultivated. The results showed that the CD4+Foxp3+ cell population and IL-10 secretion were increased by P5 treatment, suggesting that P5 inhibited Th2 and Th17 immune responses through Treg induction. In addition, in our OVA-induced food allergy model, the population of CD4+Foxp3+ cells and TGF-β secretion in mLNs from the P5-administrated group were increased compared with those in the sham group, and the levels of Th2-related cytokines and the Th17- related cytokine IL-17 were decreased compared with those in the sham group.
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