Bacteriophages of Acidovorax citrulli, the causal agent of bacterial fruit blotch, were isolated from 39 watermelon, pumpkin, and cucumber leaf samples collected from various regions of Korea and tested against 18 A. citrulli strains. Among the six phages isolated, ACP17 forms the largest plaque, an...
Bacteriophages of Acidovorax citrulli, the causal agent of bacterial fruit blotch, were isolated from 39 watermelon, pumpkin, and cucumber leaf samples collected from various regions of Korea and tested against 18 A. citrulli strains. Among the six phages isolated, ACP17 forms the largest plaque, and exhibits the morphology of phages in the Myoviridae family with a head diameter of $100{\pm}5nm$ and tail length of $150{\pm}5nm$. ACP17 has eclipse and latent periods of $25{\pm}5min$ and $50{\pm}5min$, respectively, and a burst size of 120. The genome of ACP17 is 156,281 base pairs with a G + C content of 58.7%, 263 open reading frames, and 4 transfer RNA genes. Blast search and phylogenetic analysis of the major capsid protein showed that ACP17 has limited homology to two Stentrophomonas phages, suggesting that ACP17 is a new type of Myoviridae isolated from A. citrulli.
Bacteriophages of Acidovorax citrulli, the causal agent of bacterial fruit blotch, were isolated from 39 watermelon, pumpkin, and cucumber leaf samples collected from various regions of Korea and tested against 18 A. citrulli strains. Among the six phages isolated, ACP17 forms the largest plaque, and exhibits the morphology of phages in the Myoviridae family with a head diameter of $100{\pm}5nm$ and tail length of $150{\pm}5nm$. ACP17 has eclipse and latent periods of $25{\pm}5min$ and $50{\pm}5min$, respectively, and a burst size of 120. The genome of ACP17 is 156,281 base pairs with a G + C content of 58.7%, 263 open reading frames, and 4 transfer RNA genes. Blast search and phylogenetic analysis of the major capsid protein showed that ACP17 has limited homology to two Stentrophomonas phages, suggesting that ACP17 is a new type of Myoviridae isolated from A. citrulli.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
, 2017). In this study, we isolated and characterized bacteriophages that infect A. citrulli for the first time, which can be used bacteriophage biocontrol of BFB.
Similarly, the stability of ACP17 was tested at different temperatures by incubating the phage (approximately 4.0 × 105 PFU/ml) for 24 h at –20, 4, 25, 34, 37, 45, and 60℃ before performing plaque assays.
4. Comparative analysis of phage open reading frames (ORFs) encoded by phage ACP17 and Stenotrophomonas phage IME-SM1 using using the Easyfig 9 program (http://mjsull.github.io/Easyfig/). Arrows indicate the ORFs and their orientation and lines indicate ORFs with amino acid sequence homology.
대상 데이터
Samples for phage isolation were collected from 96 sites around the Korean Peninsula. Samples included soil from watermelon farms, and leaves of watermelon, pumpkin, and cucumber plants.
Ashelford KE Day MJ Fry JC 2003 Elevated abundance of bacteriophage infecting bacteria in soil Appl Environ Microbiol 69 285 289 10.1128/AEM.69.1.285-289.2003 152412 --> 12514006
Bhunchoth A Phironrit N Leksomboon C Chatchawankanphanich O Kotera S Narulita E Kawasaki T Fujie M Yamada T 2015 Isolation of Ralstonia solanacearum-infecting bacteriophages from tomato fields in Chiang Mai, Thailand, and their experimental use as biocontrol agents J Appl Microbiol 118 1023 1033 10.1111/jam.12763 25619754
Bonilla N Rojas MI Cruz GNF Hung SH Rohwer F Barr JJ 2016 Phage on tap-a quick and efficient protocol for the preparation of bacteriophage laboratory stocks PeerJ 4 e2261 10.7717/peerj.2261 4975003 --> 27547567
Borah PK Jindal JK Verma JP 2000 Integrated management of bacterial leaf spot of mungbean with bacteriophages of Xav and chemicals J Mycol Plant Pathol 30 19 21
Buttimer C McAuliffe O Ross RP Hill C O’Mahony J Coffey A 2017 Bacteriophages and bacterial plant diseases Front Microbiol 8 34 10.3389/fmicb.2017.00034 5247434 --> 28163700
Hopkins DL 1991 Chemical control of bacterial fruit blotch of watermelon Proc Fla State Hortic Soc 104 270 272
Hopkins DL Cucuzza JD Watterson JC 1996 Wet seed treatments for the control of bacterial fruit blotch of watermelon Plant Dis 80 529 532 10.1094/PD-80-0529
Hopkins DL Lovic B Hilgren J Thompson CM 2003 Wet seed treatment with peroxyacetic acid for the control of bacterial fruit blotch and other seedborne diseases of watermelon Plant Dis 87 1495 1499 10.1094/PDIS.2003.87.12.1495
Isakeit T Black MC Barnes LW Jones JB 1997 First report of infection of honeydew with Acidovorax avenae subsp. citrulli Plant Dis 81 694 694 10.1094/PDIS.1997.81.6.694C
Jończyk E Kłak M Międzybrodzki R Górski A 2011 The influence of external factors on bacteriophages Folia Microbiol (Praha) 56 191 200 10.1007/s12223-011-0039-8 21625877
Kubota M Hagiwara N Shirakawa T 2012 Disinfection of seeds of cucurbit crops infested with Acidovorax citrulli with dry heat treatment J Phytopathol 160 364 368 10.1111/j.1439-0434.2012.01913.x
Lessl JT Fessehaie A Walcott RR 2007 Colonization of female watermelon blossoms by Acidovorax avenae ssp. citrulli and the relationship between blossom inoculum dosage and seed infestation J Phytopathol 155 114 121 10.1111/j.1439-0434.2007.01204.x
Lim JA Jee S Lee DH Roh E Jung K Oh C Heu S 2013 Biocontrol of Pectobacterium carotovorum subsp. carotovorum using bacteriophage PP1 J Microbiol Biotechnol 23 1147 1153 10.4014/jmb.1304.04001 23727798
Peng Y Leung HC Yiu SM Chin FY 2012 IDBA-UD: a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth Bioinformatics 28 1420 1428 10.1093/bioinformatics/bts174 22495754
Schaad NW Postnikova E Sechler A Claflin LE Vidaver AK Jones JB Agarkova I Ignatov A Dickstein E Ramundo BA 2008 Reclassification of subspecies of Acidovorax avenae as A. Avenae (Manns 1905) emend., A. cattleyae (Pavarino, 1911) comb. nov., A. citrulli Schaad et al., 1978) comb. nov., and proposal of A. oryzae sp. nov Syst Appl Microbiol 31 434 446 10.1016/j.syapm.2008.09.003 18993005
Sharma S Chatterjee S Datta S Prasad R Dubey D Prasad RK Vairale MG 2017 Bacteriophages and its applications: an overview Folia Microbiol 62 17 55 10.1007/s12223-016-0471-x 27718043
Song WY Kim HM Son IY Kang YK 1991 Pseudomonas pseudoalcaligenes subsp. citrulli: The causal agent of bacterial fruit blotch rot on watermelon Korean J Plant Pathol 7 177 18
Tao C Guo-Liang Q Xiao-Li Y Jun-Yi M Bai-Shi H Feng-Quan L 2009 Detection of a quorum sensing signal molecule of Acidovorax avenae subsp. citrulli and its regulation of pathogenicity Chin J Agric Biotechnol 6 49 53 10.1017/S1479236209002514
Torsvik V Øvreås L 2002 Microbial diversity and function in soil: from genes to ecosystems Curr Opin Microbiol 5 240 245 10.1016/S1369-5274(02)00324-7 12057676
Turner D Wand ME Sutton JM Centron D Kropinski AM Reynolds DM 2016 A viable prophage isolated from Acinetobacter baumannii strain A118 Genome announc 4 e01051 16 10.1128/genomeA.01051-16 5064099 --> 27738026
Walmagh M Boczkowska B Grymonprez B Briers Y Drulis-Kawa Z Lavigne R 2013 Characterization of five novel endolysins from Gram-negative infecting bacteriophages Appl Microbiol Biotechnol 97 4369 4375 10.1007/s00253-012-4294-7 22832988
Willems A Goor M Thielemans S Gillis M Kersters K De Ley J 1992 Transfer of several phytopathogenic Pseudomonas species to Acidovorax as Acidovorax avenae subsp. avenae subsp. nov., comb. nov., Acidovorax avenae subsp. citrulli, Acidovorax avenae subsp. cattleyae, and Acidovorax konjaci Int J Syst Evol Microbiol 42 107 119
Zivanovic M Walcott RR 2016 Further characterization of genetically distinct groups of Acidovorax citrulli strains Phytopathology 107 29 35 10.1094/PHYTO-06-16-0245-R 27618192
※ AI-Helper는 부적절한 답변을 할 수 있습니다.