[논문]Zymomonas mobilis를 이용한 목질계 에탄올 생산을 위한 균주 개선에 관한 연구 동향

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doi:10.5352/jls.2019.29.1.135

[국내논문] Zymomonas mobilis를 이용한 목질계 에탄올 생산을 위한 균주 개선에 관한 연구 동향
Recent Progress in Strain Development of Zymomonas mobilis for Lignocellulosic Ethanol Production 원문보기

생명과학회지 = Journal of life science, v.29 no.1 = no.225, 2019년, pp.135 - 145

초록
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자이모모나스 모빌리스(Zymomonas mobilis)는 수십 년 동안 생화학적 발효 기술을 통한 수송용 에탄올을 생산하기에 적합한 산업용 미생물로 각광을 받아왔다. 최근 이 균주의 포스트 게놈 시대 도래 및 미국 듀폰사(DuPont, USA)의 세계 최대 산업용 목질계 에탄올 생산 시설 완료 등은, 이 미생물을 이용한 산업적 에탄올 생산 공정 가시화를 위한 다양한 연구들을 파생시키고 있다. 특히, 산업용 셀룰로오스 에탄올 발효공정에 이용되는 미생물은 다양한 독성 발효 저해물질 및 물리적 스트레스에 보다 쉽게 노출 될 수 있다. 따라서 본 논문은 이 미생물이 보유한 최신 생리학적 이해와 관련 된 정보와 다양한 환경적 스트레스에 견딜 수 있는 산업적 강건성 및 산업용 균주 개발 방법에 대한 사례 및 이 균주를 이용한 가격 경쟁적인 목질계 에탄올 생산 공정 개발에 필요한 균주 개발에 대한 미래 지향적 연구 방향에 대하여 기술하였다.

Abstract ▼ AI-Helper

Zymomonas mobilis has been recognized as a potential industrial ethanologen for many decades due to its outstanding fermentation characteristics, including high ethanol tolerance, fast sugar uptake rate, and high theoretical ethanol yield. With the emergence of the postgenomic era and the recent announcement of DuPont's world largest cellulosic ethanol production process, research on this bacterium has become even more important to harness successful application not only for use in the bioethanol process but also in other biochemical processes, which can be included in bio-refinery. As an important industrial microorganism, Z. mobilis will likely be exposed to various stressful environments, such as toxic chemicals, including the end-product ethanol and fermentative inhibitory compounds (e.g., furan derivatives, organic acids, and lignin derivatives in pretreatment steps), as well as physical stresses, such as high temperature during large-scale ethanol fermentation. This review focuses on recent information related to the industrial robustness of this bacterium and strain development to improve the ethanol yield and productivity in the lignocellulosic ethanol process. Although several excellent review articles on the strain development of this bacterium have been published, this review aims to fill gaps in the literature by highlighting recent advances in physiological understanding of this bacterium that may aid strain developments and improve the ethanol productivity for lignocellulosic biomass.

Keyword

표/그림 (6)

표 Table 1. The list of Z. mobilis strains genome sequenced
표 Table 2. The list of heterologous genes expressed in Z. mobilis to broaden its substrate usages for ethanol production
표 Table 3. Effect of lignocellulosic inhibitory compounds on specific rates of xylose uptake, ethanol production, and ethanol yield of Z. mobilis ZM4 (pZB5) at 30℃ and initial pH 6 investigated by Kim et al. [25]
그림 Fig 1. A) Transmission electron microscopic image of flagellated cells of the wild type strain Z. mobilis ZM4; B) flocculent cells of the mutant strain ZM401 image stained by calcofluor white, the source adapted from Jeon et al. [22].
그림 Fig 2. Schematic diagram of cell flocculating mechanims in Z. mobilis ZM401 caused by a single point mutation in phophodiesterase domain which allow to increase intracellular level of c-di-GMP. The increased intracellular c-di-GMP subsequently results in increase in cellulose synthsis and decrease in motility. The increased cellulose synthesis metabolism with increased cell flocculation phenotype finally contributed to the enhanced volumatric ethanol productivity due to the acetate and vanillin resistance. DGC: Diguanyl cylase; PDE: Phophodiesterase.
표 Table 4. List of genes from Z. mobilis involved in stress responds confirmed by various techniques

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문제 정의

Recent research activities on this bacterium has contributed to the increased potential for further industrial application used in other chemical production [50, 53]. This paper reviews the relatively recent information related to strain development and physiological characteristics associated with this bacterium.

성능/효과

[51] also investigated gene related to acetate toxicity using a xylose/arabinose fermenting strain 8b. Their transcriptome results also suggested that the presence of acetate caused the genes related to biosynthesis, the flagellar system, and glycolysis to be downregulated, and genes related to stress responses and energy metabolism to be upregulated. Among the several gene candidates, the gene knockout mutant of TonB-dependent receptor (ZMO0128) is associate with acetate tolerance, although its function needs to be further understood.
cerevisiae DQ1, but the strain showed the stronger tolerance to the phenolic acids originated from corncob hydrolysates including 2-furoic acid, p-hydroxybenzoic acid, p-coumaric acid, vanillic acid, ferulic acid, and syringic acid. The results indicated that this bacterium has the capability for in situ detoxification of phenolic aldehydes together with lipopolysaccharide on the cell outer membrane of this bacterium providing the permeable barrier for such phenolic acids. In this respect, Yi et al.
In this regard, Na⁺⁺ tolerant strain of ZMT2 have been isolated by Wang et al [46]. The authors demonstrated that the strain of salt tolerance against Na⁺⁺ can be achieved via himA gene (ZMO1122 encoding integration host factor) disruption via Tn5-based transposon mutagenesis. Among the isolates, the mutant strain ZMT2 with improved salt tolerance phenotype was obtained in the presence of high amount of Na⁺⁺.

참고문헌 (66)

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저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

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