Bacteriophages of Acidovorax citrulli, the causal agent of bacterial fruit blotch, were isolated from 39 watermelon, pumpkin, and cucumber leaf samples collected from various regions of Korea and tested against 18 A. citrulli strains. Among the six phages isolated, ACP17 forms the largest plaque, an...
Bacteriophages of Acidovorax citrulli, the causal agent of bacterial fruit blotch, were isolated from 39 watermelon, pumpkin, and cucumber leaf samples collected from various regions of Korea and tested against 18 A. citrulli strains. Among the six phages isolated, ACP17 forms the largest plaque, and exhibits the morphology of phages in the Myoviridae family with a head diameter of $100{\pm}5nm$ and tail length of $150{\pm}5nm$. ACP17 has eclipse and latent periods of $25{\pm}5min$ and $50{\pm}5min$, respectively, and a burst size of 120. The genome of ACP17 is 156,281 base pairs with a G + C content of 58.7%, 263 open reading frames, and 4 transfer RNA genes. Blast search and phylogenetic analysis of the major capsid protein showed that ACP17 has limited homology to two Stentrophomonas phages, suggesting that ACP17 is a new type of Myoviridae isolated from A. citrulli.
Bacteriophages of Acidovorax citrulli, the causal agent of bacterial fruit blotch, were isolated from 39 watermelon, pumpkin, and cucumber leaf samples collected from various regions of Korea and tested against 18 A. citrulli strains. Among the six phages isolated, ACP17 forms the largest plaque, and exhibits the morphology of phages in the Myoviridae family with a head diameter of $100{\pm}5nm$ and tail length of $150{\pm}5nm$. ACP17 has eclipse and latent periods of $25{\pm}5min$ and $50{\pm}5min$, respectively, and a burst size of 120. The genome of ACP17 is 156,281 base pairs with a G + C content of 58.7%, 263 open reading frames, and 4 transfer RNA genes. Blast search and phylogenetic analysis of the major capsid protein showed that ACP17 has limited homology to two Stentrophomonas phages, suggesting that ACP17 is a new type of Myoviridae isolated from A. citrulli.
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제안 방법
4. Comparative analysis of phage open reading frames (ORFs) encoded by phage ACP17 and Stenotrophomonas phage IME-SM1 using using the Easyfig 9 program (http://mjsull.github.io/Easyfig/). Arrows indicate the ORFs and their orientation and lines indicate ORFs with amino acid sequence homology.
, 2017). In this study, we isolated and characterized bacteriophages that infect A. citrulli for the first time, which can be used bacteriophage biocontrol of BFB.
Similarly, the stability of ACP17 was tested at different temperatures by incubating the phage (approximately 4.0 × 105 PFU/ml) for 24 h at –20, 4, 25, 34, 37, 45, and 60℃ before performing plaque assays.
대상 데이터
Samples for phage isolation were collected from 96 sites around the Korean Peninsula. Samples included soil from watermelon farms, and leaves of watermelon, pumpkin, and cucumber plants.
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