[논문]화장품에서 1,3-부틸렌 글라이콜 및 알칸디올계 조성에 따른 방부력에 관한 연구

서지영; 윤믿음; 이예슬; 현송화; 박동순; 박수남

doi:10.15230/scsk.2018.44.4.363

화장품에서 1,3-부틸렌 글라이콜 및 알칸디올계 조성에 따른 방부력에 관한 연구
Preservative Efficacies according to the Composition of 1, 3-Butylene Glycol and Alkane Diols in Cosmetics 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.44 no.4, 2018년, pp.363 - 373

서지영 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융복합산업지원센터) , 윤믿음 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융복합산업지원센터) , 이예슬 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융복합산업지원센터) , 현송화 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융복합산업지원센터) , 박동순 (아람휴비스(주)) , 박수남 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 코스메틱 융복합산업지원센터)

초록
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최근 화장품에서 방부제로 사용되는 파라벤류는 인체 안전성에 대한 문제가 이슈화되고 있다. 따라서 본 연구에서는 파라벤류를 대체할 수 있는 방부시스템으로 1,3-butylene glycol, 1,2-hexanediol 및 1,2-pentanediol의 함량에 따른 방부력 효능을 평가하고자 하였다. 화장품 크림에 1,3-butylene glycol을 5- 25% 사이의 농도로 첨가하였다. 1,3-Butylene glycol의 방부력은 Personal Care Products Council(CTFA)의 M-3 시험법으로 측정하였다. 알칸 디올계인 1,2-hexanediol 및 1,2-pentanediol도 유사한 방법으로 평가하였다. 1,3-Butylene glycol의 함량에 따른 방부력 평가 결과, 25%를 첨가한 크림 처방에서 모든 시험 균주에 대하여 방부력을 나타내었으며, phenoxyethanol 0.3%와 ethylhexylglycerin 0.1%가 혼합된 처방에서 방부력을 나타내었다. 방부제인 phenoxyethanol의 0.3% 함량을 대체할 수 있는 대체 방부제로 alkane diol계인 1,2-hexanediol과 1,2-pentanediol을 선정하여 방부력 평가를 진행하였다. 1,2-Hexanediol과 1,2-pentanediol의 조성에 따른 방부력 평가 결과, 1,2-hexanediol 1%와 1,2-pentanediol 1%의 혼합 처방에서 방부력을 나타내었다. 결과적으로 본 연구에서는 25%의 1,3-butylene glycol과 0.1%의 ethylhexylglycerin, 1%의 1,2-hexanediol 및 1%의 1,2-pentanediol의 처방은 가장 우수한 방부력을 나타냄을 입증하였다. 따라서 이러한 처방은 화장품에서 사용되어 안전성의 이슈가 되어온 파라벤류 방부제를 대체할 수 있는 가능성이 있음을 시사한다.

Abstract ▼ AI-Helper

In recent years, parabens used as preservatives in cosmetics have become a problem of human safety. Therefore, in this study, we tried to evaluate the preservative efficacy of 1,3-butylene glycol, 1,2-hexanediol, and 1,2-pentanediol as a preservative system to replace parabens. 1,3-Butylene glycol was added to cosmetic creams at a concentration of between 5 and 25%. The preservative efficacy of 1,3-butylene glycol was determined using a M-3 challenge test, as recommended by the Personal Care Products Council (formally CTFA). The alkane diols, such as 1,2-hexanediol and 1,2-pentanediol, were assessed in a similar manner. An evaluation of the preservative efficacy of 1,3-butylene glycol revealed that it was effective against all tested microbial strains at a concentration of 25%. We also investigated the efficacy of combinations of 0.3% phenoxyethanol and 0.1% ethylhexylglycerin. Finally, we tested the alkane diols, including 1,2-hexanediol and 1,2-pentanediol, as an alternative to the preservative 0.3% phenoxyethanol. Both 1% 1,2-hexanediol and 1% 1,2-pentanediol demonstrated preservative efficacy. Taken together, our study demonstrated that the formulation of 25% 1,3-butylene glycol and 0.1% ethylhexylglycerin, 1% 1,2-hexanediol, and 1% 1,2-pentanediol had the best preservative efficacy of the compositions tested. Thus, this study suggests that the formulation is a possibility of substituting parabens preservatives, which has been used in cosmetics and has become a safety issue.

주제어

표/그림 (10)

표 Table 1. List of Strains and The Cultivation Conditions used for The Antimicrobial Experiments
표 Table 2. Formulations for The Testing of 1,3-BG
표 Table 3. Formulations Used for The Testing of Phenoxyethanol and Ethylhexylglycerin
표 Table 4. Formulations for The Testing of Alkanediols
그림 Figure 1. Efficacy of various 1,3-BG concentrations on the microbial counts in cosmetic cream. (a) S. aureus, (b) E. coli, (c) P. aeruginosa, (d) C. albicans, and (e) A. niger. NC (1,3-BG 0%); A (1,3-BG 5%); B (1,3-BG 10%); C (1,3-BG 15%); D (1,3-BG 20%); E (1,3-BG 25%).
표 Table 5. Log Reduction in Microbial Counts for Creams Containing Different 1,3-BG Concentrations
그림 Figure 2. Efficacy on microbial counts of adding phenoxyethanol and ethylhexylglycerin to cosmetic cream. (a) S. aureus, (b) E. coli, (c) P. aeruginosa, (d) C. albicans, and (e) A. niger. A (1,3-BG 5%); A-1 (ethylhexylglycerin 0.1%); A-2 (phenoxyethanol 0.3%); A-3 (ethylhexylglycerin 0.1% + phenoxyethanol 0.3%).
표 Table 6. Log Reduction in Microbial Counts for Creams Containing Phenoxyethanol and Ethylhexylglycerin
그림 Figure 3. Efficacy on microbial counts for various alkanediol compositions. (a) S. aureus, (b) E. coli, (c) P. aeruginosa, (d) C. albicans, and (e) A. niger. A-1 (ethylhexylglycerin 0.1%), E-1 (ethylhexylglycerin 0.1% + 1,2-hexanediol 1%), E-2 (ethylhexylglycerin 0.1% + 1,2-pentanediol 1%), E-3 (ethylhexylglycerin 0.1% + 1,2-hexanediol 0.5% + 1,2-pentanediol 0.5%), E-4 (ethylhexylglycerin 0.1% + 1,2-hexanediol 0.7% + 1,2-pentanediol 0.7%), and E-5 (ethylhexylglycerin 0.1% + 1,2-hexanediol 1% + 1,2-pentanediol 1%).
표 Table 7. Log Reduction in Microbial Counts for Creams with Different Alkanediol Compositions

AI 본문요약
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제안 방법

In this study, various cosmetic formulations were created with different compositions of 1,3-BG, 1,2-hexanediol, and 1,2-pentanediol. We then evaluated the preservative efficacies of these formulations against various microbes.
As any ingredient can affect final preservative efficacy, it is essential to verify the overall preservative efficacy of a new formulation after it changes. Our study was therefore conducted to aid development of a formulation free from known as preservatives that instead used safer alternatives, although the results of our evaluation are only applicable to the precise formulations described in the study.

대상 데이터

The strains used for the challenge test were the aerobic gram positive bacterium S. aureus (ATCC6538), two aerobic gram negative bacteria, E. coli (ATCC8739) and P. aeruginosa (ATCC9027), and two fungi, C. albicans (ATCC10231) and A. niger (ATCC16404). These five strains were provided by the Korean Culture Center of Microorganisms (KCCM, Seoul, Korea).

이론/모형

The challenge protocol used to assess preservative efficacy was based on the M-3 method recommended by the Personal Care Products Council (formally CTFA). Briefly, 30 g of cream was inoculated with a 1% microbial suspension (10 8 CFU/mL for bacteria or 10 7 CFU/mL for fungi).

성능/효과

In conclusion, we have identified two novel preservative-free formulations, E (25% 1,3-butylene glycol) and E-5 (1% 1,2-hexanediol and 1% 1,2-pentanediol with 0.1% ethylhexylglycerin). Based on our results, we suggest that could be applied to the creation of new preservative-free formulations for use in the cosmetic industry.
niger. Therefore, based on the M-3 method, the formulation with the best preservative efficacy was E-5 (ethylhexylglycerin 0.1% + 1,2-hexanediol 1% + 1,2-pentanediol 1%).
Figure 1 demonstrates the preservative efficacy that different concentrations of 1,3-BG had on five microbial strains, while Table 5 reveals the log reduction values for the first week after inoculation. These data reveal that 99.9% of S. aureus were killed by formulation A (1,3-BG 5%), B (1,3-BG 10%), C (1,3-BG 15%), D (1,3-BG 20%), and E (1,3-BG 25%), relative to NC (1,3-BG 0%). For E.

후속연구

We then evaluated the preservative efficacies of these formulations against various microbes. As a result of this evaluation, we assess the possibility of constructing a preservative-free formulations for cosmetics based on using 1,3-BG and alkane diols.

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