Kim, A Hyeon
(Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
,
Jang, Ji Hun
(Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
,
Woo, Kyeong Wan
(Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
,
Park, Jong Eel
(Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
,
Lee, Ki Ho
(Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
,
Jung, Ho Kyung
(Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
,
An, Byeongkwan
(Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
,
Jung, Won Seok
(Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
,
Ham, Seong Ho
(Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
,
Cho, Hyun Woo
(Division of Traditional Korean Medicine Resource, National Development Institute of Korea M)
Column chromatographic separation of the MeOH extract from the roots of Dicentra spectabilis yielded fourteen compounds, menisdaurin (1), menisdaurilide (2), trans-N-p-coumaroyltyramine (3), trans-N-p-feruloyltyramine (4), 4-O-feruloylquinicacid (5), chlorogenic acid (6), 3-O-feruloylquinicacid (7),...
Column chromatographic separation of the MeOH extract from the roots of Dicentra spectabilis yielded fourteen compounds, menisdaurin (1), menisdaurilide (2), trans-N-p-coumaroyltyramine (3), trans-N-p-feruloyltyramine (4), 4-O-feruloylquinicacid (5), chlorogenic acid (6), 3-O-feruloylquinicacid (7), ferulic acid (8), protopine (9), Kaempferol 3,7-di-O-${\beta}-{\text\tiny{D}}$-glucopyranoside (10), kaempferol 3-O-${\beta}-{\text\tiny{D}}$-glucopyranosyl-7-O-${\alpha}-{\text\tiny{L}}$-rhamnopyranoside (11), ${\alpha}-rhamnoisorobin$ (12), astragalin (13), and nicotiflorin (14). Their structures were determined on the basis of NMR spectroscopic data. Among them, compound 1, 3-8, and 10-14 isolated from this plant were reported for the first time. The isolated compounds (1-14) were tested for nitric oxide (NO) inhibitory activity on lipopolysaccharide-stimulated RAW 264.7 cells. Compound 3, 4 and 12 significantly inhibited NO production. Moreover, Compound 3 suppressed pro-inflammatory cytokines ($TNF-{\alpha}$, $IL-1{\beta}$ and IL-6) in a dose- dependent manner. These data suggest that compound 3 possess anti-inflammatory activity and might be useful natural materials for development of anti-inflammatory agent.
Column chromatographic separation of the MeOH extract from the roots of Dicentra spectabilis yielded fourteen compounds, menisdaurin (1), menisdaurilide (2), trans-N-p-coumaroyltyramine (3), trans-N-p-feruloyltyramine (4), 4-O-feruloylquinicacid (5), chlorogenic acid (6), 3-O-feruloylquinicacid (7), ferulic acid (8), protopine (9), Kaempferol 3,7-di-O-${\beta}-{\text\tiny{D}}$-glucopyranoside (10), kaempferol 3-O-${\beta}-{\text\tiny{D}}$-glucopyranosyl-7-O-${\alpha}-{\text\tiny{L}}$-rhamnopyranoside (11), ${\alpha}-rhamnoisorobin$ (12), astragalin (13), and nicotiflorin (14). Their structures were determined on the basis of NMR spectroscopic data. Among them, compound 1, 3-8, and 10-14 isolated from this plant were reported for the first time. The isolated compounds (1-14) were tested for nitric oxide (NO) inhibitory activity on lipopolysaccharide-stimulated RAW 264.7 cells. Compound 3, 4 and 12 significantly inhibited NO production. Moreover, Compound 3 suppressed pro-inflammatory cytokines ($TNF-{\alpha}$, $IL-1{\beta}$ and IL-6) in a dose- dependent manner. These data suggest that compound 3 possess anti-inflammatory activity and might be useful natural materials for development of anti-inflammatory agent.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
First, in order to get knowledge the anti-inflammatory effect in raw 264.7 cells, we investigated cytotoxic levels of compounds 1-14 using an MTS assay. MTS, an indicator of dehydrogenase activity, is commonly used to measure cell viability and proliferation (Dunigan et al.
대상 데이터
All the compounds were purified on an Agilent A1200 series HPLC (Agilent Technologies, Santa Clara, CA, USA) using a Phenomenex Luna C18-100A column (25 cm×3 mm, particle size =5 μm).
spectabilis were collected in Yeongwol, Gangwon province, Korea in June 2015. The plant was identified by professor Hui Kim (Mokpo National University, Mokpo, Korea). A voucher specimen (TKM-2095) of this plant was deposited in the Herbarium at the National Development Institute of Korea Medicine.
데이터처리
Significant differences between treated groups were determined using the Student's t-test.
Significant differences between treated groups were determined using the Student’s t-test.
All data are presented as the mean ± SD. Statistically significant differences between two group were determined by t-test using SPSS version 21 (Chicago, IL, USA). Statistical significance:*p <0.
이론/모형
The culture supernatant was collected and analyzed TNF-α, IL-6, and IL-1β using enzyme-linked immunosorbent assay (ELISA).
참고문헌 (29)
Audran G, Mori K (1998) Synthesis of phyllanthurinolactone, the leaf-closing factor of Phyllanthus urinaria, and its three stereoisomers. Eur J Org Chem 1998: 57-62
Bradding P, Roberts JA, Britten KM, Montefort S, Djukanovic R, Mueller R, Heusser CH, Howarth PH, Holgate ST (1994) Interleukin-4, -5, and -6 and tumor necrosis factor-alpha in normal and asthmatic airways: Evidence for the human mast cell as a source of these cytokines. Am J Respir Cell Mol Biol 10: 471-480
Beutler B, Greenwald D, Hulmes JD, Chang M, Pan YC, Mathison J, Ulevitch R, Cerami A (1985) Identity of tumor necrosis factor and the macrophage-secreted factor cachectin. Nature 316: 552-554
Cha BC, Lee HE (2007) Antioxidant activities of flavonoids from the leaves of Smilax china Linne. Kor J Pharmacogn 38: 31-36
Choi HS, Cho JY, Jin MR, Lee YG, Kim SJ, Ham KS, Moon JH (2016) Phenolics, acyl galactopyranosyl glycerol, and lignan amides from Tetragonia tetragonioides (Pall.) Kuntze. Food Sci Biotechnol 25: 1275-1281
deRojas-Walker T, Tamir S, Ji H, Wishnok JS, Tannenbaum SR (1995) NO induces oxidative damage in addition to deamination in macrophage DNA. Chem Res Toxicol. 8: 473-477
Dunigan DD, Waters SB, Own TC (1995) Owen Aqueous Soluble Tetrazolium/Formazan MTS as an Indicator of NADH- and NADPHDependent Dehydrogenase Activity. Bio Techniques 19: 640-649
Ge F, Ke C, Tang W, Yang X, Tang C, Qin G, Xu R, Li T, Chen X, Zuo J, Ye Y (2007) Isolation of chlorogenic acids and their derivatives from Stemona japonica by preparative HPLC and evaluation of their anti-AIV (H5N1) activity in vitro. Phytochem Anal 18: 213-218
Jang JH, Lee KH, Jung HK, Sim MO, Kim TM, Woo KW, An BK, Cho JH, Cho HW (2016) Anti-inflammatory effects of 6-O-acetyl mangiferin from Iris rossii Baker via NF-kb signal blocking in lipopolysaccharidestimulated RAW 264.7 cells. Chem Biol Interact 257: 54-60
Lee HE, Kim JA, Whang WK (2017) Chemical constituents of Smilax china L. stems and their inhibitory activities against glycation, aldose reductase, $\alpha$ -glucosidase, and lipase. Molecules 22: 451/1-451/18
Lee IK, Kim KH, Choi SU, Lee JH, Lee KR (2009) Phytochemical constituents of Thesuim chinensis TURCZ and their cytotoxic activities in vitro. Nat Prod Sci 15: 246-249
Lee KS, Sim OK, Shin JS, Choi YE, Kim EY (2004) Mass propagation of Dicentra spectabilis L. Lemaire through in vitro suspension culture. Korean J Plant Biotechnol 31: 121-126
McNulty J, Poloczek J, Larichev V, Werstiuk NH, Griffin C, Pandey S (2007) Discovery of the apoptosis-inducing activity and high accumulation of the butenolides, menisdaurilide and aquilegiolide, in Dicentra spectabilis. Planta Med 73:1543-1547
Ritchlin CT, Haas-Smith SA, Li P, Hicks DG, Schwarz EM (2003) Mechanisms of TNF-alpha- and RANKL-mediated osteoclastogenesis and bone resorption in psoriatic arthritis. J Clin Invest 111: 821-831
Seger C, Strum S, Strasser EM, Ellmerer E, Stuppner H (2004) $^1H$ and $^{13}C$ NMR signal assignment of benzylisoquiniline alkaloids from Fumaria officinalis L. (Papaveraceae). Magn Reson Chem 42: 882-886
Seigler DS, Pauli GF, Frohlich R, Wegelius E, Nahrstedt A, Glander KE, Ebinger JE (2005) Cyanogenic glycosides and menisdaurin from Guazuma ulmifolia, Ostrya virginiana, Tiquilia plicata, and Tiquilia canescens. Phytochemistry 66: 1567-1580
Sim MO, Lee HJ, Jang JH, Lee HE, Jung HK, Kim TM, No JH, Junh JK, Jung DE, Cho HW (2017) Anti-inflammatory and antioxidant effects of Spiraea prunifolia Sieb. Et Zucc. var. simpliciflora Nakai in RAW 264.7 cells. Korean J. Plant Res 30:335-342
Sim OK, Lee KS, Kim EY, Eun JS (2005) Formation of gametophytes and development of zygotic embryo in Dicentra spectabilis. Korean J Plant Res 18: 302-308
Tran TTT, Nguyen TY, Tran MH, Weon KY, Woo MH, Min BS (2014) Compounds from the aerial parts of Aceriphyllum rossii and their cytotoxic activity. Nat Prod Sci 20: 146-151
Woo KW, Lee KH, Jang JH, Kim MS, Cho HW, Cho JH, An B (2016) Antiinflammatory constituents from the aerial parts of Iris minutiaurea. Nat Prod Commun 11: 817-819
Zhao JW, Chen DS, Deng CS, Wang Q, Zhu W, Lin L (2017) Evaluation of anti-inflammatory activity of compounds isolated from the rhizome of Ophiopogon japonicas. BMC Complement Altern Med 17: 7
※ AI-Helper는 부적절한 답변을 할 수 있습니다.