[논문]Diversity of Endophytic Fungi Isolated from Pinus densiflora and Juniperus rigida Distributed in Mt. Baekryeonsan and Mt. Johangsan, Korea

Eo, Ju-Kyeong; Park, Hyeok; Eom, Ahn-Heum

doi:10.4489/kjm.20180048

Diversity of Endophytic Fungi Isolated from Pinus densiflora and Juniperus rigida Distributed in Mt. Baekryeonsan and Mt. Johangsan, Korea 원문보기

한국균학회지 = The Korean journal of mycology, v.46 no.4, 2018년, pp.437 - 446

Eo, Ju-Kyeong (Department of Biology Education, Korea National University of Education) , Park, Hyeok (Department of Biology Education, Korea National University of Education) , Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)

Abstract ▼ AI-Helper

We investigated the biodiversity of endophytic fungi in Pinus densiflora and Junipreus rigida from Mt. Baekryeonsan and Mt. Johangsan in Korea. In total, 59 isolates were discovered from 26 host plants and identified using sequences from the rDNA internal transcribed spacer (ITS) region. Twenty taxa of endophytic fungi were identified in this study; 62% of isolates belonged to Leotiomycetes, 34% belonged to Sordariomyetes, and 4% belonged to Agaricomycetes. Results confirmed three previously unreported endophytic fungi in Korea: Phanerochaete concrescens, Creosphaeria sassafras, and Scopuloides rimosa. Community analysis showed that the fungal diversity patterns of the samples from Mt Baekryeonsan and Mt. Johangsan are similar to those from previous studies and varied depending upon host and locality.

주제어

표/그림 (7)

표 Table 1. Molecular identifcation and the closest taxa of representative isolates using ITS rDNA region from Pinus densifora and Junipreus rigida in Mt. Baekryeonsan and Johangsan
그림 Fig. 1. Neighbor-joining phylogenic tree of endophytic fungi from Pinus densiflora and Junipreus rigida in Mt. Baekryeonsan and Johangsan. Internal transcribed spacer regions were used these sequence analysis to confirm the topological appropriation of isolates (1,000 replicates). Sphatularia flavida was used as an out-group.
그림 Fig. 2. Colonies of strain 11F005 (Phanerochaete concrescens) grown for 7 days on PDA (A) and MEA (D). Colonies of strain 11G047 (Creosphaeria sassafras) grown for 7 days on PDA (B) and MEA (E). Colonies of strain 11G092 (Scopuloides rimosa) grown for 7 days on PDA (C) and MEA (F). PDA, potato dextrose agar; MEA, malt extract agar.
그림 Fig. 3. Neighbor-joining phylogenetic tree based on a large subunit rDNA sequences. Donkia pulcherrima was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Fungal strain isolated in this study is in bold.
그림 Fig. 4. Neighbor-joining phylogenetic tree based on a large subunit rDNA (A) and ß-tubulin gene (B) sequences. Neoidriella desertorum and Fusarium biseptatum were used as an outgroup in each tree. Numbers on branches indicate bootstrap values (1,000 replicates). Fungal strain isolated in this study is in bold.
그림 Fig. 5. Neighbor-joining phylogenetic tree based on a large subunit rDNA sequences. Panus conchatus was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Fungal strain isolated in this study is in bold.
그림 Fig. 6. Use of Bray-Curtis ordination to validate the effective factors that contribute the endophytic fungal community. Radiating lines are host plants and locality. Axis 1 and 2 have 90.16 % explanatory power.

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제안 방법

The polymerase chain reaction products were sequenced using a 3730XL DNA analyzer (Applied Biosystems, FosterCity, CA, USA) at SolGent, Korea. Analyzed sequences were identified on NCBI using BLAST, and neighbor-joining trees were generated using MEGA7 [13] to confirm the topological position among closest taxa with bootstrap analysis (1,000 replicates). Biodiversity and community structures of endophytic fungi were analyzed according to host plants and locality.
Baekryeonsan and Johangsan. Internal transcribed spacer regions were used these sequence analysis to confirm the topological appropriation of isolates (1,000 replicates). Sphatularia flavida was used as an out-group.

성능/효과

Clampless hyphae is a specific characteristic of the genus Scopuloides [19]. Based on sequence analysis, the ITS region had 96% similarity with S. rimosa KP135350.1. LSU also showed 99% similarity with S.
[16]. Based on the sequence analysis, the ITS region had 99% similarity with the reference P. concrescens KP994380.1. Large subunit (LSU) also showed 99% similarity with the reference P.

후속연구

Thus, Lophodermiumspp. are expected to be a crucial component offungal endophytes in host plants, and additional comparative studies of the functional roles of endophytes and conifer host plants are needed.

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