[논문]쇠비름(Portulaca oleracea L.) 추출물의 탄수화물 소화 효소 저해와 식후 고혈당 완화 효과

박재은; 한지숙

doi:10.5352/jls.2018.28.4.421

쇠비름(Portulaca oleracea L.) 추출물의 탄수화물 소화 효소 저해와 식후 고혈당 완화 효과
Portulaca oleracea L. Extract Lowers Postprandial Hyperglycemia by Inhibiting Carbohydrate-digesting Enzymes 원문보기

생명과학회지 = Journal of life science, v.28 no.4 = no.216, 2018년, pp.421 - 428

초록
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식후고혈당은 제 2형 당뇨병의 발병에 부정적인 영향을 미치고 미세혈관 및 대혈관 질환 등의 당뇨병 합병증 유발과 밀접한 관계가 있다. 따라서 식후고혈당을 조절하는 것이 당뇨병 합병증의 위험을 줄이는 가장 중요한 요소이다. 식후고혈당은 소장에서 ${\alpha}$-글루코시다아제와 같은 탄수화물 소화 효소를 저해함으로써 조절될 수 있다. 이에 본 연구는 쇠비름 에탄올 추출물(PEE)과 물 추출물(PWE)이 탄수화물 소화 효소를 저해하고, 당뇨병 마우스에서 식후 고혈당을 강하시키는 효과에 대해 조사하였다. ${\alpha}$-글루코시다아제와 ${\alpha}$-아밀라아제에 대한 저해효과는 두 추출물 모두 양성대조군인 acarbose보다 더 효과적이었으며, PEE에 의한 ${\alpha}$-글루코시다아제 저해 효과가 PWE 보다 더 효과적이었다. Diabetic mice에 전분(2 g/kg)을 투여한 후의 혈당 증가는 30, 60, 120분에 각각 383.7, 429.3, 360.2 mg/dL로 나타났고, 전분(2 g/kg)과 PEE 또는 PWE 추출물(300 mg/Kg)을 투여한 후의 혈당 증가는 30, 60, 120분에 각각 337.0, 368.5, 290.1 mg/dL과 365.8, 379.2, 324.3 mg/dL로 나타나, PEE 추출물 투여군이 대조군에 비해 식후 혈당 강하가 효과적으로 나타남을 알 수 있었다. 이러한 결과는 쇠비름 추출물이 탄수화물 소화효소를 저해함으로써 식후 고혈당을 완화시키고, 특히 쇠비름 에탄올 추출물(PEE)이 쇠비름 물 추출물(PWE) 보다 식후 고혈당을 완화시키는데 더욱 효과가 있는 것으로 나타났다.

Abstract ▼ AI-Helper

Postprandial hyperglycemia plays an important role in the development of Type 2 Diabetes and diabetic complications. Controlling postprandial hyperglycemia is the most important factor for reducing the risks of diabetic complications in Type 2 diabetic patients. This study was designed to determine whether Portulaca oleracea L. extract suppresses the activation of carbohydrate-digesting enzymes, and lowers postprandial hyperglycemia in diabetic mice through streptozotocin. P. oleracea was extracted with either 80% ethanol (PEE) or water (PWE), and the extract solutions were concentrated. The ${\alpha}$-glucosidase and ${\alpha}$-amylase inhibition assays were performed using the chromogenic method. Normal mice and STZ-induced diabetic mice were orally treated with PEE, PWE (300 mg/kg of body weight) or acarbose (100 mg/kg of body weight), with soluble starch (2 g/kg of body weight). The ${\alpha}$-glucosidase and ${\alpha}$-amylase inhibitory effectiveness by PEE were markedly more effective than PWE, and both extracts indicated a higher effectiveness than the acarbose (positive control). The rise in postprandial blood glucose due to starch loading was markedly inhibited in the PEE group when compared to the control group in diabetic and normal mice. Furthermore, the area under the concentration-time curve values were markedly declined by the PEE injection in the diabetic group when compared to that exerted for the control group. These results demonstrate that P. oleracea extracts lower postprandial hyperglycemia by inhibiting carbohydrate-digesting enzymes, and that the ethanol extract is more efficacious than the water extract.

주제어

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문제 정의

Thus, maintaining nearly normality level of fasting and postprandial glucose is important for managing diabetes [26]. This study confirmed the anti-hyperglycemic effect of PEE and PWE in diabetic and normal group after starch ingestion by administering PEE or PWE. In diabetic mice administered PEE, post-prandial hyperglycemia significantly decreased.

가설 설정

1)IC₅₀ value is the concentration of a sample required for 50% inhibition. Each value is expressed as the mean
제안 방법
- extracts. 3T3-L1 cells were treated with various concentrations (0.1, 0.5, 1.0, and 2.0 mg/ml) of PEE and PWE for 24 hr, and cyto-toxic effects was measured by the MTT assay. Each value is expressed as the mean ± SD of triplicate experiments.
- A total of 8 groups were used and orally administered the following after overnight fasting: 1) control: soluble starch (2 g/kg of body weight [BW]), 2) PEE: soluble starch with PEE (300 mg/kg of BW), 3) PWE: soluble starch with PWE (300 mg/kg of BW), 4) acarbose: soluble starch with acarbose (100 mg/kg of BW). Blood samples were collected from the tail vein at 0, 30, 60, and 120 min, and blood glucose was checked using a blood glucose meter (Roche Diagnistics GmbH, Germany). Areas under the concentration-time curves (AUCs) were identified using the trapezoidal rule [16].
- To evaluate whether PEE and PWE have toxic effect to the cells, PEE and PWE were treated in 3T3-L1 cells for 24 hr, and cytotoxic effect was measured using MTT assay. PEE and PWE were not exerting any cytotoxic effect at the various concentrations (0.
대상 데이터
- ICR male mice (4-week-old; purchased from Orient, Inc., Seoul, Korea) used for the study were kept under a 12 hr on/12 hr off cycle and controlled room temperature. The mice were fed a pelleted feed (5L79, Orient, Inc.
데이터처리
- Dissimilarity between groups were assessed by one-way analysis of variance, followed by Duncan’s post-hoc multiple range tests.
- Values with different superscript letters within a column are significantly different (p<0.05) based on Duncan’s multiple range tests.
이론/모형
- Blood samples were collected from the tail vein at 0, 30, 60, and 120 min, and blood glucose was checked using a blood glucose meter (Roche Diagnistics GmbH, Germany). Areas under the concentration-time curves (AUCs) were identified using the trapezoidal rule [16].

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