[논문]New surveillance concepts in food safety in meat producing animals: the advantage of high throughput 'omics' technologies - A review

Pfaffl, Michael W.; Riedmaier-Sprenzel, Irmgard

doi:10.5713/ajas.18.0155

New surveillance concepts in food safety in meat producing animals: the advantage of high throughput 'omics' technologies - A review 원문보기

Asian-Australasian journal of animal sciences, v.31 no.7, 2018년, pp.1062 - 1071

Pfaffl, Michael W. (Animal Physiology and Immunology, TUM School of Life Sciences, Technical University of Munich Weihenstephan) , Riedmaier-Sprenzel, Irmgard (Animal Physiology and Immunology, TUM School of Life Sciences, Technical University of Munich Weihenstephan)

Abstract ▼ AI-Helper

The misuse of anabolic hormones or illegal drugs is a ubiquitous problem in animal husbandry and in food safety. The ban on growth promotants in food producing animals in the European Union is well controlled. However, application regimens that are difficult to detect persist, including newly designed anabolic drugs and complex hormone cocktails. Therefore identification of molecular endogenous biomarkers which are based on the physiological response after the illicit treatment has become a focus of detection methods. The analysis of the 'transcriptome' has been shown to have promise to discover the misuse of anabolic drugs, by indirect detection of their pharmacological action in organs or selected tissues. Various studies have measured gene expression changes after illegal drug or hormone application. So-called transcriptomic biomarkers were quantified at the mRNA and/or microRNA level by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) technology or by more modern 'omics' and high throughput technologies including RNA-sequencing (RNA-Seq). With the addition of advanced bioinformatical approaches such as hierarchical clustering analysis or dynamic principal components analysis, a valid 'biomarker signature' can be established to discriminate between treated and untreated individuals. It has been shown in numerous animal and cell culture studies, that identification of treated animals is possible via our transcriptional biomarker approach. The high throughput sequencing approach is also capable of discovering new biomarker candidates and, in combination with quantitative RT-qPCR, validation and confirmation of biomarkers has been possible. These results from animal production and food safety studies demonstrate that analysis of the transcriptome has high potential as a new screening method using transcriptional 'biomarker signatures' based on the physiological response triggered by illegal substances.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

This article is dedicated to Prof. Heinrich H. D. Meyer (deceased April 13, 2012) whose passion was the field of residue analysis and who always believed in the idea of gene expression biomarkers to combat the abuse of anabolic substances. A special thanks to the Kontrollgemeinschaft Deutsches Kalbfleisch, the Société Générale de Surveillance (SGS) and Denkavit for supporting our animal study.
However a previous report by Swan et al [3] described a reduction of sperm quality of male offspring that was positively correlated with the mother's consumption of beef during pregnancy. This research showed a direct connection between the residues from application of growth promotants and their activity in meat. Such metabolites can influence the development of male reproductive organs in utero, providing supporting evidence for the ban on growth promoting drugs in European animal husbandry [3].

제안 방법

This review describes new surveillance concepts for food safety in meat producing animals, by explaining the technical and analytical requirements of mRNA and microRNA gene expression profiling. Further, the advantages of the high throughput ‘omics’ technologies are described, as are efforts being made to identify transcriptional biomarkers to detect misuse of anabolic agents in cattle.

성능/효과

In a second in vivo study, it was shown that it is possible to extract RNA from hair follicle cells and that gene expression in these cells is also influenced by steroid hormones[43]. In conclusion, hair roots represent a promising tissue to find gene expression biomarkers with potential to develop a noninvasive screening method based on gene expression patterns.
To also verify these candidate markers in other species, their expression was quantified in liver samples from boars treated with Synovex plus (trenbolone acetate plus estradiol). It was shown that 4 genes were significantly regulated, with expression dynamics of a 25-fold downregulation of CYP2E1 and a 5-fold upregulation of cut like homeobox 2. The biostatistical analysis via PCA and HCA resulted in a complete separation of treated and untreated animals [28].
Candidate genes chosen for their physiological action in different biological pathways were quantified via RT-qPCR. The study identified 8 to 18 significantly regulated genes in the tissues studied, although none of these genes was up- or down-regulated more than 2-fold. In the uterus, 13 significantly regulated genes were identified, and the expression changes were up to 6-fold [30].

후속연구

Hence these tissues to not present as promising primary target tissues for developing a screening method for illicit drug administration. To enable controls on-farm, tissues or matrices that can be sampled from living individuals in a noninvasive form and independent of gender must be the focus of further research. Reiter et al [42] monitored gene expression changes in cell culture experiments with human hair follicle dermal papilla cells that were treated with stanozolol [42].

참고문헌 (47)

1 Council Directive 96/23/EC (OJ L125, p10, 23/05/1996) of 29 April 1996 on measures to monitor certain substances and residues in live animals and animal products Brussels, Belgium European Commission 1996
2 De Brabander HF Le Bizec B Pinel G Past, present and future of mass spectrometry in the analysis of residues of banned substances in meat-producing animals J Mass Spectrom 2007 42 983 98 17657726

상세보기
3 Swan SH Liu F Overstreet JW Brazil C Skakkebaek NE Semen quality of fertile US males in relation to their mothers’ beef consumption during pregnancy Hum Reprod 2007 22 1497 502 17392290

상세보기
4 Meyer HHD Hoffmann S Development of a sensitive microtitration plate enzyme-immunoassay for the anabolic steroid trenbolone Food Addit Contam 1987 4 149 60 3595922

상세보기
5 Meyer HHD Rinke L Duersch I Residue screening for the beta-agonists clenbuterol, salbutamol and cimaterol in urine using enzyme immunoassay and high-performance liquid chromatography J Chromatogr B Biomed Sci Appl 1991 564 551 6
6 Scippo ML Degand G Duyckaerts A Maghuin-Rogister G Delahaut P Control of the illegal administration of natural steroid hormones in the plasma of bulls and heifers Analyst 1994 119 2639 44 7879867

상세보기
7 Cantiello M Carletti M Cannizzo FT Effects of an illicit cocktail on serum immunoglobulins, lymphocyte proliferation and cytokine gene expression in the veal calf Toxicology 2007 242 39 51 17936467

상세보기
8 Riedmaier I Pfaffl MW Transcriptional Biomarkers - High throughput screening, quantitative verification and bioinformatical validation methods Methods 2013 59 3 9 22967906

상세보기
9 Pfaffl MW Advanced molecular diagnostics for biomarker discovery Biomol Detect Quantif 2015 5 1 2 27077036
10 Pinel G Weigel S Antignac JP Targeted and untargeted profiling of biological fluids to screen for anabolic practices in cattle Trends Analyt Chem 2010 29 1269 80
11 Riedmaier I Becker C Pfaffl MW Meyer HHD The use of omic technologies for biomarker development to trace functions of anabolic agents J Chromatogr A 2009 1216 8192 9 19233374

상세보기
12 Heneghan HM Miller N Kerin MJ MiRNAs as biomarkers and therapeutic targets in cancer Curr Opin Pharmacol 2010 10 543 50 20541466

상세보기
13 Kurian KM Watson CJ Wyllie AH DNA chip technology J Pathol 1999 187 267 71 10398077
14 Weile J Knabbe C Current applications and future trends of molecular diagnostics in clinical bacteriology Anal Bioanal Chem 2009 394 731 42 19377839

상세보기
15 Wang Z Gerstein M Snyder M RNA-Seq: a revolutionary tool for transcriptomics Nat Rev Genet 2009 10 57 63 19015660

상세보기
16 Yamamoto M Wakatsuki T Hada A Ryo A Use of serial analysis of gene expression (SAGE) technology J Immunol Methods 2001 250 45 66 11251221

상세보기
17 Spornraft M Haase B Benes V Pfaffl MW Riedmaier I Optimization of extraction of circulating RNAs from plasma – enabling small RNA sequencing PLoS One 2014 9 e107259 25229651

상세보기
18 Costa V Angelini C De F Ciccodicola A Uncovering the complexity of transcriptomes with RNA-Seq J Biomed Biotechnol 2010 2010 Article ID 853916
19 Spornraft M Kirchner B Pfaffl MW Riedmaier I The potential of circulating extracellular small RNAs (smexRNA) in veterinary diagnostics – identifying biomarker signatures by multivariate data analysis Biomol Detect Quantif 2015 5 15 22 27077039
20 Buschmann D Haberberger A Kirchner B Towards reliable biomarker signatures in the age of liquid biopsies – how to standardize the small RNA-Seq workflow Nucleic Acids Res 2016 44 5995 6018 27317696
21 Alwine JC Kemp DJ Stark GR Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes Proc Natl Acad Sci USA 1977 74 5350 4 414220

상세보기
22 Higuchi R Dollinger G Walsh PS Griffith R Simultaneous amplification and detection of specific DNA-sequences Biotechnology (NY) 1992 10 413 7
23 Kubista M Andrade JM Bengtsson M The real-time polymerase chain reaction Mol Aspects Med 2006 27 95 125 16460794

상세보기
24 Smith CJ Osborn AM Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology FEMS Microbiol Ecol 2009 67 6 20 19120456

상세보기
25 Gunson RN Bennett S Maclean A Carman WF Using multiplex real time PCR in order to streamline a routine diagnostic service J Clin Virol 2008 43 372 5 18977692

상세보기
26 Meyer SU Kaiser S Wagner C Thirion C Pfaffl MW Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs – a comparative study PLoS ONE 2012 7 e38946 22723911

상세보기
27 Becker C Riedmaier I Reiter M Changes in the miRNA profile under the influence of anabolic steroids in bovine liver Analyst 2011 136 1204 9 21212882

상세보기
28 Riedmaier I Benes V Blake J RNA-sequencing as useful screening tool in the combat against the misuse of anabolic agents in cattle Anal Chem 2012 84 6863 8 22861009
29 Ginsburg GS Haga SB Translating genomic biomarkers into clinically useful diagnostics Expert Rev Mol Diagn 2006 6 179 91 16512778

상세보기
30 Reiter M Walf VM Christians A Pfaffl MW Meyer HHD Modification of mRNA expression after treatment with anabolic agents and the usefulness for gene expression-biomarkers Anal Chim Acta 2007 586 73 81 17386698

상세보기
31 Lopparelli RM Zancanella V Giantin M Steroidogenic enzyme gene expression profiles in the testis of cattle treated with illicit growth promoters Steroids 2011 76 508 16 21295601

상세보기
32 Becker C Riedmaier I Reiter M Influence of anabolic combinations of an androgen plus an estrogen on biochemical pathways in bovine uterine endometrium and ovary J Steroid Biochem Mol Biol 2010 125 192 201
33 Riedmaier I Reiter M Tichopad A Pfaffl MW Meyer HHD The potential of bovine vaginal smear for biomarker development to trace the misuse of anabolic agents Exp Clin Endocrinol Diabetes 2011 119 86 94 21031338

상세보기
34 De Jager N Hudson NJ Reverter A Chronic exposure to anabolic steroids induces the muscle expression of oxytocin and a more than fiftyfold increase in circulating oxytocin in cattle Physiol Genomics 2011 43 467 78 21325062
35 Toffolatti L Rosa GL Patarnello T Expression analysis of androgen-responsive genes in the prostate of veal calves treated with anabolic hormones Domest Anim Endocrinol 2006 30 38 55 16023321

상세보기
36 Pfaffl MW Daxenberger A Hageleit M Meyer HHD Effects of synthetic progestagens on the mRNA expression of androgen receptor, progesterone receptor, estrogen receptor alpha and beta, insulin-like growth factor (IGF)-1 and IGF-1 receptor in Heifer tissues J Vet Med A Physiol Pathol Clin Med 2002 49 57 64 11958468
37 Sato S Nomura S Kawano F Effects of the beta2-agonist clenbuterol on beta1- and beta2-adrenoceptor mRNA expressions of rat skeletal and left ventricle muscles J Pharmacol Sci 2008 107 393 400 18678986
38 Becker C Riedmaier I Reiter M Effect of trenbolone acetate plus estradiol on transcriptional regulation of metabolism pathways in bovine liver Horm Mol Biol Clin Investig 2011 2 257 65
39 Riedmaier I Spornraft M Pfaffl MW Identification of a potential gene expression biomarker signature in bovine liver to detect the abuse of growth promoters Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2014 4 641 9
40 Giantin M Lopparelli RM Zancanella V Effects of illicit dexamethasone upon hepatic drug metabolizing enzymes and related transcription factors mRNAs and their potential use as biomarkers in cattle J Agric Food Chem 2010 58 1342 9 20041653

상세보기
41 Rijk JC Peijnenburg AA Hendriksen PJ Feasibility of a liver transcriptomics approach to assess bovine treatment with the prohormone dehydroepiandrosterone (DHEA) BMC Vet Res 2010 6 44 20846423

상세보기
42 Reiter M Pfaffl MW Schönfelder M Meyer HHD Gene expression in hair follicle dermal papilla cells after treatment with stanozolol Biomark Insights 2009 4 1 8

상세보기
43 Reiter M Lüderwald S Pfaffl MW Meyer HHD First steps towards new screening method anabolic androgenic androgens in human hair follicles Doping J 2008 5 3
44 Riedmaier I Tichopad A Reiter M Pfaffl MW Meyer HHD Identification of potential gene expression biomarkers for the surveillance of anabolic agents in bovine blood cells Anal Chim Acta 2009 638 106 13 19298887

상세보기
45 Lee G Rodriguez C Madabhushi A Investigating the efficacy of nonlinear dimensionality reduction schemes in classifying gene and protein expression studies IEEE/ACM Trans Comput Biol Bioinform 2008 5 368 84 18670041
46 Bergkvist A Rusnakova V Sindelka R Gene expression profiling--Clusters of possibilities Methods 2010 50 323 35 20079843

상세보기
47 Costa V Angelini C De Feis I Ciccodicola A Uncovering the complexity of transcriptomes with RNA-Seq J Biomed Biotechnol 2010 2010 Article ID853916

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증