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Inhibition of MMP-2 and MMP-9 activities by solvent-partitioned Sargassum horneri extracts

Fisheries and aquatic sciences, v.21 no.6, 2018년, pp.16.1 - 16.7  

Karadeniz, Fatih (Department of Organic Material Science and Engineering, Pusan National University) ,  Lee, Seul-Gi (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University) ,  Oh, Jung Hwan (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University) ,  Kim, Jung-Ae (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University) ,  Kong, Chang-Suk (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University)

Abstract AI-Helper 아이콘AI-Helper

Background: Matrix metalloproteinases (MMPs) are linked with several complications such as metastasis of cancer progression, oxidative stress, and hepatic fibrosis. Brown seaweeds are being extensively studied for their bioactive molecule content against cancer progression. In this context, Sargassu...

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제안 방법

  • However, treatment with SHE was observed to produce mixed results regarding the effect on the TIMP levels following the PMA stimuli. Expected results were to inhibit MMP expression while enhancing TIMP expression in order to regulate the extracellular matrix degradation. In these terms, only 85% aq.
  • FK and SGL carried out the experiments. JAK, and CSK conceived the idea and designed the experiments.
  • The target cDNA was amplified using the following primers: forward 5′-TGA-AGG-TCG-GTG-TGA-ACG-GA-3′ and reverse 5′-CAT-GTA-GCC-ATG-AGG-TCC-ACC-AC-3′ for MMP-2; forward 5′-CAC-TGT-CCA-CCC-CTC-AG A-GC-3′ and reverse 5′-CAC-TTG-TCG-GCG-ATA-AG G-3′ for MMP-9; forward 5′-AAT-TCC-GAC-CTC-GTCATC-AG-3′ and reverse 5′-TGC-AGT-TTT-CCA-GCAATG-AG-3′ for TIMP-1; forward 5′-TGA-TCC-ACA-CA C-GTT-GGT-CT-3′ and reverse 5′-TTT-GAG-TTG-CT T-GCA-GGA-TG-3′ for TIMP-2; and forward 5′-GCC-A CC-CAG-AAG-ACT-GTG-GAT-3′ and reverse 5′-TGGTCC-AGG-GTT-TCT-TAC-TCC-3′ for β-actin.

대상 데이터

  • JAK, and CSK conceived the idea and designed the experiments. FK, JHO and JAK participated to write the manuscript and interpreted the data. JHO performed the necessary revisions and updates on the manuscript.

데이터처리

  • Differences between the calculated means of the each individual group were determined by one-way ANOVA coupled with Duncan’s multiple range tests.

이론/모형

  • The cells were treated with different concentrations of SHEs (5 and 50 μg/ml) and incubated for 48 h. The cytotoxicity was determined by MTT assay. Values are mean ± SD (n = 3).
  • Total protein contents of the cells were normalized using Bradford protein determination method.
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참고문헌 (22)

  1. Bauvois B. New facets of matrix metalloproteinases MMP-2 and MMP-9 as cell surface transducers: outside-in signaling and relationship to tumor progression. Biochim Biophys Acta Rev Cancer. 2012;1825:29-36. 

  2. Benjamin MM, Khalil RA. Matrix metalloproteinase inhibitors as investigative tools in the pathogenesis and management of vascular disease. EXS. 2012;103: 209-79. 

  3. Bhatnagar I, Kim SK. Marine antitumor drugs: status, shortfalls and strategies. Mar Drugs. 2010;8:2702-20. 

  4. de la Mare JA, Lawson JC, Chiwakata MT, Beukes DR, Edkins AL, Blatch GL. Quinones and halogenated monoterpenes of algal origin show antiproliferative effects against breast cancer cells in vitro. Investig New Drugs. 2012;30:2187-200. 

  5. Egeblad M, Werb Z. New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer. 2002;2:161-74. 

  6. Holdt SL, Kraan S. Bioactive compounds in seaweed: functional food applications and legislation. J Appl Phycol. 2011;23:543-97. 

  7. Ibanez E, Cifuentes A. Benefits of using algae as natural sources of functional ingredients. J Sci Food Agric. 2013;93:703-9. 

  8. Jiao J, Friedman SL, Aloman C. Hepatic fibrosis. Curr Opin Gastroenterol. 2009;25: 223-9. 

  9. Jones JL, Walker RA. Control of matrix metalloproteinase activity in cancer. J Pathol. 1997;183:377-9. 

  10. Kato H, Duarte S, Liu D, Busuttil RW, Coito AJ. Matrix metalloproteinase-2 (MMP-2) gene deletion enhances MMP-9 activity, impairs PARP-1 degradation, and exacerbates hepatic ischemia and reperfusion injury in mice. PLoS One. 2015; 10(9):e0137642. 

  11. Kim YA, Kong CS, Park HH, Lee E, Jang MS, Nam KH, Seo Y. Anti-inflammatory activity of heterocarpin from the salt marsh plant Corydalis heterocarpa in LPS-induced RAW 264.7 macrophage cells. Molecules. 2015;20:14474-86. 

  12. Matanjun P, Mohamed S, Mustapha NM, Muhammad K. Nutrient content of tropical edible seaweeds, Eucheuma cottonii, Caulerpa lentillifera and Sargassum polycystum. J Appl Phycol. 2009;21:75-80. 

  13. Moss LAS, Jensen-Taubman S, Stetler-Stevenson WG. Matrix metalloproteinases: changing roles in tumor progression and metastasis. Am J Pathol. 2012;181: 1895-9. 

  14. Overall CM, Lopez-Otin C. Strategies for MMP inhibition in cancer: innovations for the post-trial era. Nat Rev Cancer. 2002;2:657-72. 

  15. Patarra RF, Paiva L, Neto AI, Lima E, Baptista J. Nutritional value of selected macroalgae. J Appl Phycol. 2011;23:205-8. 

  16. Plaza M, Santoyo S, Jaime L, Reina GGB, Herrero M, Senorans FJ. Screening for bioactive compounds from algae. J Pharm Biomed Anal. 2010;51:450-5. 

  17. Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: how are they linked? Free Radic Biol Med. 2010;49:1603-16. 

  18. Seo Y, Lee HJ, Park KE, Kim YA, Ahn JW, Yoo JS, Lee BJ. Peroxynitrite-scavenging constituents from the brown alga Sargassum thunbergii. Biotechnol Bioprocess Eng. 2004;9:212-6. 

  19. Shipeng Y, Woo HC, Choi JH, Park YB, Chun BS. Measurement of antioxidant activities and phenolic and flavonoid contents of the brown seaweed Sargassum horneri: comparison of supercritical $CO_2$ and various solvent extractions. Fish Aquat Sci. 2015;18:123-30. 

  20. Thomas NV, Kim SK. Potential pharmacological applications of polyphenolic derivatives from marine brown algae. Environ Toxicol Pharmacol. 2011;32: 325-35. 

  21. Tu G, Xu W, Huang H, Li S. Progress in the development of matrix metalloproteinase inhibitors. Curr Med Chem. 2008;15:1388-95. 

  22. Yu P, Gu H. Bioactive substances from marine fishes, shrimps and algae and their functions: present and future. Crit Rev Food Sci Nutr. 2015;55:1114-36. 

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