시설재배 키위 중 carbendazim과 diethofencarb 혼합제의 잔류특성 Residual Characteristics of Fungicides Mixture of Carbendazim and Diethofencarb in Kiwifruit under Greenhouse Condition원문보기
본 연구는 시설재배 키위를 대상으로 carbendazim과 diethofencarb 혼합살균제의 잔류특성을 분석하고, 그에 따른 안전사용기준 설정의 기초자료를 확보하고자 수행하였다. Carbendazim과 diethofencarb의 잔류량은 시험약제(carbendazim diethofencarb 수화제, 25%+25%) 1000배 희석액을 시설재배지 키위에 수확일을 기준으로 14일과 21일전에 7~10일 간격으로 2~3회에 거쳐 살포한 후 수확한 키위를 대상으로 두 성분을 각각 분석하여 산출하였으며, 각 성분의 잔류량 분석에는 HPLC-FLD와 HPLC-UVD를 이용하였다. 그 결과, carbendazim과 diethofencarb의 정량한계는 각각 0.04와 0.025 mg/kg 이었고, 회수율은 각각 110.3~114.7%와 91.8~94.6%, 저장안정성은 각각 102.2%와 100.9% 이었으며, 회수율 및 저장안정성의 변이계수는 모두 10% 미만이었다. 시험구 키위에서 carbendazim의 잔류량은 0.11~0.26 mg/kg 범위였으며, diethofencarb의 잔류량은 0.67~1.15 mg/kg 범위였다. 이는 현재 고시되어 있는 carbendazim과 diethofencarb의 국내 잔류허용기준(3.0 mg/kg) 보다 낮은 수준이었다. 이러한 결과는 키위에 대한 carbendazim과 diethofencarb 혼합살균제의 안전사용기준을 설정하는데 중요한 기초자료가 될 것으로 사료된다.
본 연구는 시설재배 키위를 대상으로 carbendazim과 diethofencarb 혼합살균제의 잔류특성을 분석하고, 그에 따른 안전사용기준 설정의 기초자료를 확보하고자 수행하였다. Carbendazim과 diethofencarb의 잔류량은 시험약제(carbendazim diethofencarb 수화제, 25%+25%) 1000배 희석액을 시설재배지 키위에 수확일을 기준으로 14일과 21일전에 7~10일 간격으로 2~3회에 거쳐 살포한 후 수확한 키위를 대상으로 두 성분을 각각 분석하여 산출하였으며, 각 성분의 잔류량 분석에는 HPLC-FLD와 HPLC-UVD를 이용하였다. 그 결과, carbendazim과 diethofencarb의 정량한계는 각각 0.04와 0.025 mg/kg 이었고, 회수율은 각각 110.3~114.7%와 91.8~94.6%, 저장안정성은 각각 102.2%와 100.9% 이었으며, 회수율 및 저장안정성의 변이계수는 모두 10% 미만이었다. 시험구 키위에서 carbendazim의 잔류량은 0.11~0.26 mg/kg 범위였으며, diethofencarb의 잔류량은 0.67~1.15 mg/kg 범위였다. 이는 현재 고시되어 있는 carbendazim과 diethofencarb의 국내 잔류허용기준(3.0 mg/kg) 보다 낮은 수준이었다. 이러한 결과는 키위에 대한 carbendazim과 diethofencarb 혼합살균제의 안전사용기준을 설정하는데 중요한 기초자료가 될 것으로 사료된다.
This study was carried out to investigate residual characteristics of fungicides mixture of carbendazim and diethofencarb in kiwifruit under greenhouse condition and consequentially to obtain basic data for guideline on safe use of this fungicides mixture in kiwi. The tested fungicides mixture (carb...
This study was carried out to investigate residual characteristics of fungicides mixture of carbendazim and diethofencarb in kiwifruit under greenhouse condition and consequentially to obtain basic data for guideline on safe use of this fungicides mixture in kiwi. The tested fungicides mixture (carbendazim diethofencarb wettable powder, 25%+25%) were diluted at 1,000 times and sprayed 2~3 times onto the kiwi with 7~10 days interval, and then fruits were collected at 14 and 21 days after final application. The residues of carbendazim and diethofencarb in kiwifruit were analyzed by HPLC-FLD and HPLC-UVD, respectively. Limits of quantitation of carbendazim and diethofencarb were 0.04 and 0.025 mg/kg, respectively. The recoveries of carbendazim and diethofencarb were ranged to 110.3~114.7% and 91.8~94.6%, respectively, with below 10% of coefficient of variation. The storage stabilities of carbendazim and diethofencarb were 102.2% and 100.9%, respectively, with below 10% of coefficient of variation. The residues of carbendazim and diethofencarb in all treatment samples were ranged to 0.11~0.26 and 0.67~1.15 mg/kg, respectively. These levels were lower than maximum residue limit (3.0 mg/kg) in kiwifruit. These results might be basic information to construct guideline for safe use of fungicides mixture of carbendazim and diethofencarb in kiwi.
This study was carried out to investigate residual characteristics of fungicides mixture of carbendazim and diethofencarb in kiwifruit under greenhouse condition and consequentially to obtain basic data for guideline on safe use of this fungicides mixture in kiwi. The tested fungicides mixture (carbendazim diethofencarb wettable powder, 25%+25%) were diluted at 1,000 times and sprayed 2~3 times onto the kiwi with 7~10 days interval, and then fruits were collected at 14 and 21 days after final application. The residues of carbendazim and diethofencarb in kiwifruit were analyzed by HPLC-FLD and HPLC-UVD, respectively. Limits of quantitation of carbendazim and diethofencarb were 0.04 and 0.025 mg/kg, respectively. The recoveries of carbendazim and diethofencarb were ranged to 110.3~114.7% and 91.8~94.6%, respectively, with below 10% of coefficient of variation. The storage stabilities of carbendazim and diethofencarb were 102.2% and 100.9%, respectively, with below 10% of coefficient of variation. The residues of carbendazim and diethofencarb in all treatment samples were ranged to 0.11~0.26 and 0.67~1.15 mg/kg, respectively. These levels were lower than maximum residue limit (3.0 mg/kg) in kiwifruit. These results might be basic information to construct guideline for safe use of fungicides mixture of carbendazim and diethofencarb in kiwi.
Koh, Y. J., J. G. Lee, D. H. Lee and J. S. Hur (2003a) Botryspheria dothidea, the causal organism of ripe rot of kiwifruit (Actinidia deliciosa) in Korea. Plant Pathol. J. 19(5):227-230.
Koh, Y. J., J. G. Lee, J. S. Hur and J. S. Jung (2003b) Incidences and causal agents of postharvest fruit rots in kiwifruits in Korea. Res. Plant Dis. 9(4):196-200.
KCPA (2018) Korea Crop Protection Association. http://koreacpa.org/index3/guide/sub03.php.
Lee, Y. D. (2012) Practical book of pesticide residue analysis method of food code. 3rd ed. National Institution of Food and Drug Safety Evaluation, Korea.
Michailides, T. and P. Elmer (2000) Botrytis gray mold of kiwifruit caused by Botrytis cinerea in the United States and New Zealand. Plant Disease. 84(3):208-223.
MFDS (2017) Pesticide MRLs in Food. Ministry of Food and Drug Safety. pp. 359.
NIHHS (2017) Status of Domestic Kiwi Industry. National Institute of Horticultural & Herbal Science. http://www.nihhs.go.kr/usr/main/mainPage.do.
Paik, J. C., B. J. Cha and Y. J. Koh (1993a) Ecological investigation of occurrence of major diseases and insects in kiwifruit: current state of culture and occurence of diseases and insects in kiwifruit. Sunchon Natl. Univ. Bull. 12(1):75-82.
Paik, J. C., B. J. Cha and Y. J. Koh (1993b) Ecological investigation of occurrence of major diseases and insects in kiwifruit: symptoms, pathogens and ecology of major diseases in kiwifruit. Sunchon Natl. Univ. Bull. 12(1):83-93.
Qin, X., X. Yingming, S. Yang, Z. Lijie, W. Lin, S. Yuebing and L. Xuefeng (2016) Determination of Carbendazim and Diethofencarb in Cotton and Soil by High-Performance Liquid Chromatography. Analytical Letters. 49(10):1631-1639.
Rural Development Administration (RDA) Notification No. 1997-3 (1997) Guideline of test for pesticide registration.
Rural Development Administration (RDA) Publication registration. No. 11-1390804-000249-01 (2012) Countermeasures in competitiveness improvement by agricultural item in response to FTA.
Williamson, B., B. Tudzynski, P. Tudzynski and J. A. L. Van Kan (2007) Botrytis cinerea: the cause of grey mould disease. Mol. Plant Pathol. 8(5):561-580.
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