Oh, Kyo-nyeo
(Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research)
,
Oh, Dool-Ri
(Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research)
,
Jung, Myung-A
(Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research)
,
Kim, Yujin
(Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research)
,
Choi, Eun Jin
(Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research)
,
Hong, Ji Ae
(Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research)
,
Kim, Jaeyong
(Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research)
,
Choi, Chul-yung
(Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research)
A This study evaluated the antidepressant effects of the herbal mixture CPAE(Cynanchum wilfordii Hemsley, Phlomis umbrosa Turcz, and Angelica gigas Nakai) using several tests, including a test for serotonin 6($5-HT_6$) receptor activity, the forced swimming test(FST), and tests for cortic...
A This study evaluated the antidepressant effects of the herbal mixture CPAE(Cynanchum wilfordii Hemsley, Phlomis umbrosa Turcz, and Angelica gigas Nakai) using several tests, including a test for serotonin 6($5-HT_6$) receptor activity, the forced swimming test(FST), and tests for corticosterone(CORT) and monoamine levels. CPAE showed antagonistic effects on the $5-HT_6$ receptor in a stable $5-HT_6$ receptor-expressing cell line. We subsequently confirmed the antidepressant effects of CPAE in chronic stress model in mice and explored the underlying mechanisms of its action. Specifically, we observed that CPAE treatment significantly reduced immobility time in the FST and effectively restored abnormal levels of CORT in plasma and of monoamines(serotonin, dopamine, and norepinephrine) in hippocampus and prefrontal cortex. These results suggest that CPAE has significant antidepressant effects.
A This study evaluated the antidepressant effects of the herbal mixture CPAE(Cynanchum wilfordii Hemsley, Phlomis umbrosa Turcz, and Angelica gigas Nakai) using several tests, including a test for serotonin 6($5-HT_6$) receptor activity, the forced swimming test(FST), and tests for corticosterone(CORT) and monoamine levels. CPAE showed antagonistic effects on the $5-HT_6$ receptor in a stable $5-HT_6$ receptor-expressing cell line. We subsequently confirmed the antidepressant effects of CPAE in chronic stress model in mice and explored the underlying mechanisms of its action. Specifically, we observed that CPAE treatment significantly reduced immobility time in the FST and effectively restored abnormal levels of CORT in plasma and of monoamines(serotonin, dopamine, and norepinephrine) in hippocampus and prefrontal cortex. These results suggest that CPAE has significant antidepressant effects.
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제안 방법
The negative control group was administered the same volume of normal saline, and the positive control group was administered 10 mg/kg escitalopram. All drugs and vehicle were administered orally using a sonde needle at 10:30 a.m. once per day for 21 consecutive days. Mice were under restraint stress continuously for 6 h(between 11:00 am and 5:00 pm) daily for 3 weeks while placed in an acrylic container with a height of 7 cm and a diameter of 3 cm.
대상 데이터
Adult SPF/VAF outbred male CrljOri:CD-1(ICR) mice(5 weeks old, 18-20) were purchased from ORIENT BIO(Sungnam, Korea). Animals were maintained at a constant room temperature of 22±2℃ with a humidity level of 50 ± 5% and with free access to water and food under a 12:12 h light : dark cycle(lights on at 8:00 am).
데이터처리
The data were statistically evaluated using Student’s t-tests or one-way analyses of variance(ANOVAs) followed by Duncan’s multiple range test to compare groups.
성능/효과
For the three types of behaviors, swimming, climbing, and immobility, durations in the control group was 16.0 ± 3.4, 13.4 ± 2.6, and 128.6 ± 14.49 s, respectively, and durations of first two behavioral types were shorter and the duration of immobility was longer than in the normal group(79.44 ± 7.60, 68.87 ± 6.65, and 83.63 ± 4.13 s, respectively).
In conclusion, CPAE showed an antagonistic activity on the 5-HT6 receptor in the in vitro model. Also, it decreased the duration of immobility during the FST component of a chronic restraint-induced stress model and effectively recovered levels of CORT and monoamine neurotransmitters which became abnormal because of restraint-induced stress.
In conclusion, this study indicated that CPAE was observed to act as a 5-HT6 receptor antagonist and to increase three monoamines(5-HT, DA, and NE) in the hippocampus and prefrontal cortex, suggesting that the antagonistic activity of CPAE on the 5-HT6 receptor is involved in ameliorating depression via the modulation of neurotransmitters.
The results showed that 3, 10, 30, 100, and 300 μg/mL CPAE showed inhibitory effects on cAMP activity of 30.99 ± 4.00, 36.89 ± 3.66, 43.22 ± 5.75, 49.40 ± 4.74, and 56.00 ± 5.22%, respectively.
Also, it decreased the duration of immobility during the FST component of a chronic restraint-induced stress model and effectively recovered levels of CORT and monoamine neurotransmitters which became abnormal because of restraint-induced stress. To conclude, CPAE improved stress-induced depression via its antagonistic activity on the 5-HT6 receptor, suggesting that CPAE can be developed into food and drug products to improve depression.
참고문헌 (38)
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