[논문]A continuous-flow and on-site mesocosm for ocean acidification experiments on benthic organisms

Kim, Ju-Hyoung; Kang, Eun Ju; Kim, Keunyong; Kim, Kwang Young

doi:10.4490/algae.2018.33.11.10

A continuous-flow and on-site mesocosm for ocean acidification experiments on benthic organisms 원문보기

Algae, v.33 no.4, 2018년, pp.359 - 366

Kim, Ju-Hyoung (Faculty of Marine Applied Biosciences, Kunsan National University) , Kang, Eun Ju (Research Institute for Basic Science, Chonnam National University) , Kim, Keunyong (Research Institute for Basic Science, Chonnam National University) , Kim, Kwang Young (Department of Oceanography, College of Natural Sciences, Chonnam National University)

Abstract ▼ AI-Helper

Mesocosm experiments conducted for ecological purposes have become increasingly popular because they can provide a holistic understanding of the biological complexities associated with natural systems. This paper describes a new outdoor mesocosm designed for $CO_2$ perturbation experiments of benthos. Manipulated the carbonate chemistry in a continuous flow-through system can be parallelized with diurnal changes, while irradiance, temperature, and nutrients can vary according to the local environment. A target hydrogen ion activity (pH) of seawater was sufficiently stabilized and maintained within 4 h after dilution, which was initiated by the ratio of $CO_2$-saturated seawater to ambient seawater. Specifically, pH and $CO_2$ partial pressure ($pCO_2$) levels gradually varied from 8.05-7.28 and $375-2,691{\mu}atm$, respectively, over a range of dilution ratios. This mesocosm can successfully manipulate the pH and $pCO_2$ of seawater, and it demonstrates suitability for ocean acidification experiments on benthic communities.

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제안 방법

Seawater pH was determined with spectrophotometric measurements using a high-resolution spectrophotometer (Agilent 8453 UV-Visible Spectrophotometer; Agilent Technologies, Pal Alto, CA, USA). Using a siphon tube, the samples were filled into a 10 cm path length cuvette without air bubbles, and then, absorbance was measured with or without adding an indicator dye at wavelengths of 434, 578, and 730 nm and a constant temperature of 25°C.

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The mesocosm was installed near the rocky shore of Namhae-gun, South Sea of Korea (34°48′30″ N, 127°49′37″ E), and consisted of cylindrical acrylic tanks (1.2 m in diameter and 1.2 m in height) that were transparent and lidless (Figs 1 & 2).

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