[논문]오가피(五加皮) 수피(樹皮)와 근피(根皮)의 MIA 유도 골관절염 흰쥐에 미치는 영향 비교

심우형; 서부일

doi:10.6116/kjh.2018.33.5.53.

오가피(五加皮) 수피(樹皮)와 근피(根皮)의 MIA 유도 골관절염 흰쥐에 미치는 영향 비교
A study on the effect comparative of acanthopanax stem bark (ASB) and acanthopanax root bark (ARB) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.33 no.5, 2018년, pp.53 - 66

심우형 (대구한의대학교 한의과대학 본초학교실) , 서부일 (대구한의대학교 한의과대학 본초학교실)

Abstract ▼ AI-Helper

Objectives : This study was designed to compare the effects of acanthopanax stem bark (ASB) and acanthopanax root bark (ARB) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods : The antioxidant activities were evaluated through radical scavenging assays using 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Also, we examined total poly phenol and flavonoids contents. Osteoarthritis was caused by injection MIA($50{\mu}{\ell}$ with $80mg/m{\ell}$) into the knee joint cavity of rats. Rats were divided by 4 groups (normal group, control group, ASB treated group, ARB treated group, each n=6). The changes in the levels of reactive oxygen species (ROS), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were analyzed after experiment. Also, the anti-oxidant, inflammatory protein levels were investigated western blot analysis. Knee joint tissue, histopathological observation hematoxylin & eosin staining and safranin-O staining were measured. Results : In the present study, ARB treated group showed superior inhibitory effects on the inflammatory parameters than the ASB treated group. ARB aqueous extract was effective in antioxidant measurements. The administration of ARB showed a significant reduction of changes in relative hind paw weight distribution. Morever, it decreased ROS, ALT and AST levels in serum, compared with those of the control rats. The ARB administration inhibited the biomarkers of inflammatory in tissues. Conclusions : ASB aqueous extract and ARB aqueous extract have a great effect on osteoarthritis, and ARB aqueous extract has excellent effect on osteoarthritis through antioxidant and anti-inflammation.

주제어

표/그림 (20)

표 Table 1. Anti-oxidant Effects of ASB and ARB
표 Table 2. Total Polyphenol and Total Flavonoid Contents of ASB and ARB
표 Table 3. Body Weight and Food Intake
표 Table 4. The Changes in Relative Hind Paw Weight Distribution in MIA-induced Osteoarthritis Rats
그림 Fig. 1. Oxidative stress biomarker (ROS) in serum. All data are expressed means ± SEM, n=6 rats per group Significance：**p < 0.01, ***p < 0.001 vs. MIA-induced osteoarthritis rats by student LSD test Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 2. Alanine transaminase (ALT) in serum. All data are expressed means ± SEM, n=6 rats per group Significance：**p < 0.01 vs. MIA-induced osteoarthritis rats by student LSD test Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 3. Aspartate transaminase (AST) in serum. All data are expressed means ± SEM, n=6 rats per group Significance：***p < 0.001 vs. MIA-induced osteoarthritis rats by student LSD test Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 4. Western blot analysis of nuclear factor erythroid 2 (Nrf2) expression; expression levels in the MIA-induced osteoarthritis rats. All data are expressed means ± SEM, n=6 rats per group There is no significant difference in the experimental groups Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 5. Western blot analysis of glutathione peroxidase (GPx) expression; expression levels in the MIA-induced osteoarthritis rats. All data are expressed means ± SEM, n=6 rats per group There is no significant difference in the experimental groups Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 6. Western blot analysis of catalase expression; expression levels in the MIA-induced osteoarthritis rats. All data are expressed means ± SEM, n=6 rats per group Significance：**p < 0.01 vs. MIA-induced osteoarthritis rats by student LSD test Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 7. Western blot analysis of Nuclear factor-kappa Bp65 (NF-kBp65) expression; expression levels in the MIA-induced osteoarthritis rats. All data are expressed means ± SEM, n=6 rats per group Significance：*p < 0.05, **p < 0.01 vs. MIA-induced osteoarthritis rats by student LSD test Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 8. Western blot analysis of p-IκBα expression; expression levels in the MIA-induced osteoarthritis rats. All data are expressed means ± SEM, n=6 rats per group Significance：*p < 0.05, **p < 0.01 vs. MIA-induced osteoarthritis rats by student LSD test Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 9. Western blot analysis of cyclooxygenase2 (COX2) expression; expression levels in the MIA-induced osteoarthritis rats. All data are expressed means ± SEM, n=6 rats per group Significance：*p < 0.05, **p < 0.01 vs. MIA-induced osteoarthritis rats by student LSD test Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 10. Western blot analysis of inducible nitric oxide synthase (iNOS) expression; expression levels in the MIA-induced osteoarthritis rats. All data are expressed means ± SEM, n=6 rats per group Significance：*p < 0.05, **p < 0.01 vs. MIA-induced osteoarthritis rats by student LSD test Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 11. Western blot analysis of tumor necrosis factor-alpha (TNF-α) expression; expression levels in the MIA-induced osteoarthritis rats. All data are expressed means ± SEM, n=6 rats per group Significance：*p < 0.05, **p < 0.01 vs. MIA-induced osteoarthritis rats by student LSD test Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 12. Western blot analysis of interleukin-16 (IL-6) expression; expression levels in the MIA-induced osteoarthritis rats. All data are expressed means ± SEM, n=6 rats per group Significance：*p < 0.05 vs. MIA-induced osteoarthritis rat by student LSD test. Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 13. Western blot analysis of NADPH oxidase 4 (NOX4) expression; expression levels in the MIA-induced osteoarthritis rats. All data are expressed means ± SEM, n=6 rats per group Significance：**p < 0.01 vs. MIA-induced osteoarthritis rat by student LSD test Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 14. Western blot analysis of p47phox expression; expression levels in the MIA-induced osteoarthritis rats. All data are expressed means ± SEM, n=6 rats per group Significance：**p < 0.01, ***p < 0.001 vs. MIA-induced osteoarthritis rats by student LSD test Nor：normal rats Con：MIA-induced osteoarthritis rats ASB：MIA-induced osteoarthritis rats treated with acanthopanax stem bark extract 200 ㎎/㎏ body weight ARB：MIA-induced osteoarthritis rats treated with acanthopanax root bark extract 200 ㎎/㎏ body weight
그림 Fig. 15. The histological analysis of the knee joint tissues after treatment of ASB and ARB in MIA-induced osteoarthritis rat (Hematoxylin and eosin staining, ✕ 200). In the Normal, synovial tissue and cartilage were well developed. In the Control, most of articular cartilage and synovial tissue were destructed. In the ASB and ARB, articular cartilage and synovial tissue were preserved, compared with Control. (A) Nor：normal rats (B) Con：MIA-induced osteoarthritis rats (C) ASB：MIA-induced osteoarthritis rats injected with acanthopanax stem bark extract 200 mg/kg body weight (D) ARB：MIA-induced osteoarthritis rats injected with acanthopanax root bark extract 200 mg/kg body weight.
그림 Fig. 16. The histological analysis of the knee joint tissues after treatment of ASB and ARB in MIA-induced osteoarthritis rat (Safranin-O staining, ✕ 200). In the Normal, there are much of proteoglycan around the cartilage. In the Control, most of proteoglycan was broken down, so red color was disappeared. In the ASB and ARM red color was around the cartilage, so we knew proteoglycan was well preserved compared with Control. (A) Nor：normal rats (B) Con：MIA-induced osteoarthritis rats (C) ASB：MIA-induced osteoarthritis rats injected with acanthopanax stem bark extract 200 mg/kg body weight (D) ARB：MIA-induced osteoarthritis rats injected with acanthopanax root bark extract 200 mg/kg body weight.

질의응답

핵심어	질문	논문에서 추출한 답변
	DPPH free radical 소거법은 어떠한 목적으로 사용되는가?	항산화 활성을 측정하는 방법으로 Blosis에 의한 DPPH free radical 소거법을 사용하였다9). 시료 중에 포함된 항산화 물질의 양을 측정하는데 사용되는 대표적인 실험법이다. 일정농 도를 가진 시료 100 ㎕와 60 μM, 그리고 DPPH 용액 100 ㎕를 넣고 혼합한 후, 실온에서 30분 동안 반응시켰다.
	골관절염이란 무엇인가?	골관절염 (osteoarthritis)은 연골의 세포외 기질 (extracellular matrix, ECM)의 손상과 연골세포의 소멸 (death of chondrocyte)을 특징으로 하는 퇴행성 질환의 한 종류이다. 골관절염 (osteoarthritis)의 연골 손상 원인은 아직 정확히 밝혀지지 않았으나, IL-1 (interleukin-1)이나 TNF (tumor necrosis factor) 등의 염증 cytokine에 의해 세포외 기질파괴가 촉진되어, 관절 연골의 손상 및 연골세포의 소멸을 야기하여 발생하는 것으로 알려져 있다1).
	골관절염의 연골 손상 원인으로 어떠한 것이 알려져 있는가?	골관절염 (osteoarthritis)은 연골의 세포외 기질 (extracellular matrix, ECM)의 손상과 연골세포의 소멸 (death of chondrocyte)을 특징으로 하는 퇴행성 질환의 한 종류이다. 골관절염 (osteoarthritis)의 연골 손상 원인은 아직 정확히 밝혀지지 않았으나, IL-1 (interleukin-1)이나 TNF (tumor necrosis factor) 등의 염증 cytokine에 의해 세포외 기질파괴가 촉진되어, 관절 연골의 손상 및 연골세포의 소멸을 야기하여 발생하는 것으로 알려져 있다1). 현대 사회의 노인인구의 비율 증가에 맞춰, 대표적 노인 질환이라 볼 수 있는 골관절염의 발병 빈도 역시 가파르게 증가하고 있어, 이에 대한 적절한 대책이 필요하다2).

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

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