Simultaneous determination of three methoxyflavones, namely, cirsimarin (1), hispidulin (2), and cirsimaritin (3) in selected Korean thistles was performed via reversed-phase high performance liquid chromatography system. Compound 1 was present in all the thistle species examined, whereas 2 and 3 we...
Simultaneous determination of three methoxyflavones, namely, cirsimarin (1), hispidulin (2), and cirsimaritin (3) in selected Korean thistles was performed via reversed-phase high performance liquid chromatography system. Compound 1 was present in all the thistle species examined, whereas 2 and 3 were only detected in Cirsium japonicum and C. japonicum var. maackii (CJM). The concentration of compounds 1-3 in CJM varied according to the time of harvest. Plants collected in the spring (CJMS) and fall (CJMF) had the highest contents of 3 and 1, respectively. A lower content of 2 was observed in CJMF than in CJMS. This indicates that seasonal variation affects the flavonoid content of CJM. The results of this study show that CJM is an excellent source of compounds 1-3 and it can potentially be cultivated for industrial and pharmaceutical applications involving these compounds.
Simultaneous determination of three methoxyflavones, namely, cirsimarin (1), hispidulin (2), and cirsimaritin (3) in selected Korean thistles was performed via reversed-phase high performance liquid chromatography system. Compound 1 was present in all the thistle species examined, whereas 2 and 3 were only detected in Cirsium japonicum and C. japonicum var. maackii (CJM). The concentration of compounds 1-3 in CJM varied according to the time of harvest. Plants collected in the spring (CJMS) and fall (CJMF) had the highest contents of 3 and 1, respectively. A lower content of 2 was observed in CJMF than in CJMS. This indicates that seasonal variation affects the flavonoid content of CJM. The results of this study show that CJM is an excellent source of compounds 1-3 and it can potentially be cultivated for industrial and pharmaceutical applications involving these compounds.
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문제 정의
These compounds have been shown to exhibit bioactive properties, and thus have pharmacological importance [15-18]. Accordingly, the aim of this study was to determine the distribution of these compounds and quantify their contents in selected Korean thistles by high performance liquid chromatography (HPLC) with ultraviolet-visible detection. The results of this study will serve as a basis for the quality evaluation and selection of Korean thistles to be cultivated for industrial and pharmaceutical applications involving these compounds.
제안 방법
HPLC analysis was performed using a Waters HPLC system equipped with a binary pump and a UV-Vis detector (Milford, MA, USA). All solvents used were HPLC grade including, MeOH, acetonitrile (ACN), and water for the analysis.
2). The peaks of all chromatograms were confirmed by spiking the HPLC samples with the reference compounds and by UV comparison for qualitative analysis. The concentrations and distributions of the methoxyflavones in the samples analyzed are summarized in Table 2.
성능/효과
Moreover, compounds 1-3 in CJM has also been reported to exhibit beneficial against menopausal symptoms in animal models [18]. Moreover, the results showed that although CJ and CJM belong to the same species, the two varieties showed distinct flavonoid contents in that all three methoxyflavones were present in CJM whereas 2 was absent in CJ. The contents of 1 and 3 were also higher in CJM than in CJ.
후속연구
Accordingly, the aim of this study was to determine the distribution of these compounds and quantify their contents in selected Korean thistles by high performance liquid chromatography (HPLC) with ultraviolet-visible detection. The results of this study will serve as a basis for the quality evaluation and selection of Korean thistles to be cultivated for industrial and pharmaceutical applications involving these compounds.
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