[논문]Phylogenetic analysis of Viburnum (Adoxaceae) in Korea using DNA sequences

CHOI, Yun Gyeong; YOUM, Jung Won; LIM, Chae Eun; OH, Sang-Hun

doi:10.11110/kjpt.2018.48.3.206

Phylogenetic analysis of Viburnum (Adoxaceae) in Korea using DNA sequences 원문보기

식물분류학회지 = Korean journal of plant taxonomy, v.48 no.3, 2018년, pp.206 - 217

CHOI, Yun Gyeong (Department of Biology, Daejeon University) , YOUM, Jung Won (Department of Biology, Daejeon University) , LIM, Chae Eun (Plant Resources Division, National Institute of Biological Resources) , OH, Sang-Hun (Department of Biology, Daejeon University)

Abstract ▼ AI-Helper

The nucleotide sequences of the chloroplast rbcL, matK, and psbA-trnH and nuclear internal transcribed spacer (ITS) regions were determined from all species of Viburnum in Korea with multiple accessions to reconstruct the phylogeny and to evaluate the utility of the DNA sequences as DNA barcodes. The results of phylogenetic analyses of the cpDNA and ITS data are consistent with the findings of previous studies of Viburnum. Four morphologically closely related species, V. dilatatum, V. erosum, V. japonicum, and V. wrightii, were included in a strongly supported sister clade of V. koreanum and V. opulus. Viburnum odoratissimum is suggested to be sister to the V. dilatatum/V. koreanum clade in the cpDNA data, while V. odoratissimum is a sister to V. furcatum in the ITS data. Viburnum burejaeticum and V. carlesii are strongly supported as monophyletic. Our analyses of DNA barcode regions from multiple accessions of the species of Viburnum in Korea confirm that six out of ten species in Korea can be discriminated at the species level. The V. dilatatum complex can be separated from the remaining species according to molecular data, but the resolution power to differentiate a species within the complex is weak. This study suggests that regional DNA barcodes are useful for molecular species identification in the case of Viburnum when flowering or fruiting materials are not available.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

This study aims to determine the DNA sequences of rbcL, matK, psbA-trnH, and ITS regions from multiple accessions of all species of Viburnum in Korea to assess the level of differentiation of the species and to evaluate the resolution power of DNA barcodes to identify a species. In addition, we provide a phylogeny of Viburnum with Korean species, onto which some important morphological characters are mapped.

제안 방법

Heuristic searches were used with 100 replicates of random sequence additions with tree bisection-reconnection branch swapping, with all of the best trees saved at each step (MulTrees).
The first 2,000 trees (200,000 generations) were discarded as “burn-in,” and the remaining trees for which the log-likelihood values had reached a plateau were imported in PAUP* to calculate the posterior probability of each clade.

이론/모형

Bayesian phylogenetic analyses were performed with the program MrBayes version 3.2.5 (Huelsenbeck and Ronquist, 2001). A Markov chain Monte Carlo algorithm was employed for 1,000,000 generations, sampling trees every 100 generations, with four independent chains running simultaneously.

성능/효과

taquetii appears to be plastic. Four to eight accessions per taxon were analyzed, except for V. koreanum, whose distribution is limited at the high elevation of Mt. Seoraksan in Korea (Table 1). Viburnum clemensiae Kern was included as an outgroup based on previous studies (Winkworth and Donoghue, 2004; Clement and Donoghue, 2012; Clement et al.
2). The results of a combined analysis of the cpDNA and ITS regions are consistent with the ITS data (Fig. 3), suggesting that phylogenetic signals from the ITS regions are stronger than cpDNA. Previous analysis of the ITS region (Donoghue et al.
The species resolution power, by which each species was differentiated in the phylogenetic tree, was calculated and found to be 60% in separate analyses of the cpDNA and ITS data and in the combined analysis. Viburnum burejaeticum, V.

참고문헌 (36)

Ahn, D. K. 1998. Illustrated Book of Korean Medicinal Herbs. Kyohaksa, Seoul, 854 pp.
Cantino, P. D., J. A. Doyle, S. W. Graham, W. S. Judd, R. G. Olmstead, D. E. Soltis, P. S. Soltis and M. J. Donoghue. 2007. Towards a phylogenetic nomenclature of Tracheophyta. Taxon 56: 822-846.

상세보기
CBOL Plant Working Group. 2009. A DNA barcode for land plants. Proceedings of the National Academy of Sciences of the United States of America 106: 12794-12797.

상세보기
Clement, W. L., M. Arakaki, P. W. Sweeney, E. J. Edwards and M. J. Donoghue. 2014. A chloroplast tree for Viburnum (Adoxaceae) and its implications for phylogenetic classification and character evolution. American Journal of Botany 101: 1029-1049.

상세보기
Clement, W. L. and M. J. Donoghue. 2011. Dissolution of Viburnum section Megalotinus (Adoxaceae) of Southeast Asia and its implications for morphological evolution and biogeography. International Journal of Plant Sciences 172: 559-573.

상세보기
Clement, W. L. and M. J. Donoghue. 2012. Barcoding success as a function of phylogenetic relatedness in Viburnum, a clade of woody angiosperms. BMC Evolutionary Biology 12: 73.

상세보기
Cuenoud, P., V. Savolainen, L. W. Chatrou, M. Powell, R. J. Grayer and M. W. Chase. 2002. Molecular phylogenetics of Caryophyllales based on nuclear 18S rDNA and plastid rbcL, atpB, and matK DNA sequences. American Journal of Botany 89: 132-144.

상세보기
Donoghue, M. J. 1983. The phylogenetic relationships of Viburnum. Advances in Cladistics 2: 143-166.
Donoghue, M. J., B. G. Baldwin, J. Li and R. C. Winkworth. 2004. Viburnum phylogeny based on chloroplast trnK intron and nuclear ribosomal ITS DNA sequences. Systematic Botany 29: 188-198.

상세보기
Donoghue, M. J., C. D. Bell and R. C. Winkworth. 2003. The evolution of reproductive characters in Dipsacales. International Journal of Plant and Science 164: S453-S464.

상세보기
Edgar, R. C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792-1797.

상세보기
Egolf, D. R. 1962. A cytological study of the genus Viburnum. Journal of the Arnold Arboretum 43: 132-172.
Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783-791.

상세보기
Hara, H. 1983. A Revision of Caprifoliaceae of Japan with Reference to Allied Plants in Other Districts and the Adoxaceae. Academia Scientific Books, Tokyo, 336 pp.
Hebert, P. D. N., A. Cywinska, S. L. Ball and J. R. deWaard. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences 270: 313-321.

상세보기
Hong, H.-H. and H.-T. Im. 2003. Viburnum japonicum (Caprifoliaceae): an unrecorded species in Korea. Korean Journal of Plant Taxonomy 33: 271-277.

원문보기 상세보기
Huelsenbeck, J. P. and F. Ronquist. 2001. MrBayes: Bayesian inference of phylogenetic tress. Bioinformatics 17: 754-755.

상세보기
Kim, H. M., S.-H. Oh, G, S. Bhandari, C.-S. Kim and C.-W. Park. 2014. DNA barcoding of Orchidaceae in Korea. Molecular Ecology Resources 14: 499-507.

상세보기
Kim, T. J. 2007. Viburnaceae Raf. In The Genera of Vascular Plants of Korea. Park C.-W. (ed.), Academy Publishing Co., Seoul. Pp. 944-946.
Kress, W. J. and D. L. Erickson. 2007. A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS ONE 2: e508.

상세보기
Kress, W. J., K. J. Wurdack, E. A. Zimmer, L. A. Weigt and D. H. Janzen. 2005. Use of DNA barcodes to identify flowering plants. Proceedings of the National Academy of Sciences of the United States of America 102: 8369-8374.

상세보기
Kwon, M. K., H. E. Lee, J. Y. Park and Y. S. Hahn. 2003. Antimicrobial activities of berry extract of domestic plants on 4 kinds of pathogenic microorganism. Journal of the East Asian Society of Dietary Life 13: 433-438.
Lahaye, R. M. van der Bank, D. Bogarin, J. Warner, F. Pupulin, G. Gigot, O. Maurin, S. Duthoit, T. G. Barraclough and V. Savolainen. 2008. DNA barcoding the floras of biodiversity hotspots. Proceedings of the National Academy of Sciences of the United States of America 105: 2923-2928.

상세보기
Mabberley, D. J. 1997. The Plant-Book. 2nd ed. Cambridge University Press, Cambridge, 680 pp.
Posada, D. and K. A. Crandall. 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817-818.

상세보기
Rehder, A. 1908. The Viburnums of eastern Asia. In Trees and Shrubs. Vol. II, Part II. Sargent, C. S. (ed.), Houghton Mifflin, Boston, MA. Pp. 105-116.
Sambrook, J., E. F. Fritzsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press, New York, 2100 pp.
Sang, T., D. Crawford and T. Stuessy. 1997. Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). American Journal of Botany 84: 1120-1136.

상세보기
Soltis, P. S., D. E. Soltis and C. J. Smiley. 1992. An rbcL sequence from a Miocene Taxodium (bald cypress). Proceedings of the National Academy of Sciences of the United States of America 89: 449-451.

상세보기
Son, H.-D., D.-H. Kim and H.-T. Im. 2008. A short record for the distribution of 4 rare plants. Korean Journal of Plant Taxonomy 38: 565-571.

원문보기 상세보기
Swofford, D. L. 2002. PAUP* Phylogenetic analysis using parsimony (* and other methods), version 4.0. Sinauer Associates, Sunderland, MA.
Winkworth, R. C. and M. J. Donoghue. 2004. Viburnum phylogeny: evidence from the duplicated nuclear gene GBSSI. Molecular Phylogenetics and Evolution 33: 109-126.

상세보기
Winkworth, R. C. and M. J. Donoghue. 2005. Viburnum phylogeny based on combined molecular data: implications for taxonomy and biogeography. American Journal of Botany 92: 653-666.

상세보기
Xiang, X.-G., H. Hu, W. Wang and X.-H. Jin. 2011. DNA barcoding of the recently evolved genus Holcoglossum (Orchidaceae: Aeridinae): a test of DNA barcode candidates. Molecular Ecology Resources 11: 1012-1021.

상세보기
Youm, J. W., S.-W. Han, S. W. Seo, C. U. Lim and S.-H. Oh. 2016. DNA barcoding of Schisandraceae in Korea. Korean Journal of Plant Taxonomy 46: 273-282.

원문보기 상세보기
Zhang, N., W. Sun and J. Yang. 2016. Chromosome counts and karyotype analysis of Viburnum taxa (Adoxaceae). Caryologia 69: 12-19.

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증