[논문]Scopolamine 유발 기억력손상 흰 쥐에서 영계감조탕가용골모려(笭桂甘棗湯加龍骨牡蠣)의 인지기능개선과 항산화 효과

김대억; 한다영; 김상호; 정대규

doi:10.7231/jon.2018.29.3.121

Scopolamine 유발 기억력손상 흰 쥐에서 영계감조탕가용골모려(笭桂甘棗湯加龍骨牡蠣)의 인지기능개선과 항산화 효과
Anti-amnesic and Antioxidant Effect of Yeongkyekamjotanggayonggolmoryo Aqueous Extracts on Scopolamine-induced Memory Impairment in Mice 원문보기

동의신경정신과학회지 = Journal of oriental neuropsychiatry, v.29 no.3, 2018년, pp.121 - 134

김대억 (대구한의대학교 한의과대학 신경정신과교실) , 한다영 (대구한의대학교 한의과대학 신경정신과교실) , 김상호 (대구한의대학교 한의과대학 신경정신과교실) , 정대규 (대구한의대학교 한의과대학 신경정신과교실)

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Objectives: The purpose of this in vivo study is to observe anti-amnesic effects of Yeongkyekamjotanggayonggolmoryo (YGYM), a novel mixed herbal prescription, Ossis Mastodi and Ostreae Testa added Yeongkyekamjo-tang, on scopolamine induced amnesia in C57BL/6 mice through acetylcholine (ACh) and acetylcholinesterase (AChE) activity, Choline acetyltransferase (ChAT) mRNA expression, and antioxidant effects. Methods: Six groups, total 20 intact or 100 Sco treated mice were used in this study after one week of acclimatization period. Half the animals were used for passive avoidance task tests and hippocampus ACh content, AChE activity, and ChAT mRNA expression were measured. The other half was subjected to an underwater maze test and then the cerebral cortex antioxidant defense system was measured. Results: In the passive avoidance experiment, there was significant decrease in residence time in the bright room and in the underwater maze test, escape latency to escape from the esophagus significantly increased compared with the normal control group. At the final sacrifice, ACh content and ChAT mRNA expression decreased, AChE activity increased, and cerebral cortical MDA increased GSH content, SOD and CAT activity in Sco control mice, as compared to intact vehicle control mice. However, these Sco treatment-related memory loss through AChE activation destroyed the cerebral cortex antioxidant defense system, and was inhibited dose-dependently by 28 days consecutive oral pretreatments of YGYM extracts 500, 250, 125 mg/kg. Conclusions: In the above results, YGYM extract that oral administration of YGYM extracts alleviates the antioxidant defense system, through preservation of ACh mediated by upregulation of ChAT mRNA expression, and increase of AChE inhibition and brain antioxidant defense systems.

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핵심어	질문	논문에서 추출한 답변
	알츠하이머가 치매를 야기하게되는 원인은?	알츠하이머환자의 뇌에서 아세틸콜린(Acetylcholine)의 감소는 치매를 일으키는 중요한 요소로 알려져있다1,2). 아세틸콜린(ACh)를 분해하는 아세틸콜린에스테라제(Acetylcholinesterase)가 계속하여 작용하면 아세틸콜린(ACh)이감소되어 기억력감퇴와 치매를 야기하게 된다3). 그래서 현재, 콜린성 가설(cholinergic hypothesis)을 바탕으로 아세틸콜린에스테라제(AChE) 억제제가 치료제로 사용되어지고 있다4).
	인지기능이란?	인지기능이란 상황을 보고, 듣고 파악하는 능력으로, 기억력, 연산능력, 판단력, 사고력 등을 말한다. 이러한 인지기능이 문제가 생기는 대표적인 퇴행성 두뇌질환이 치매이다.
	인지기능이 문제가 생기는 대표적인 퇴행성 두뇌질환은 무엇인가?	인지기능이란 상황을 보고, 듣고 파악하는 능력으로, 기억력, 연산능력, 판단력, 사고력 등을 말한다. 이러한 인지기능이 문제가 생기는 대표적인 퇴행성 두뇌질환이 치매이다. 치매에 있어서 60% 정도를 차지할 정도로 가장 많은 형태의 질환인 알츠하이머병은 처음에는 최근 일을 기억하는 것에서 문제를 보이다가 언어기능과 판단력 등 점차적으로 다른 기능상실을 만들어 인지기능저하와 신경증상을 나타낸다1).

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