[논문]Scopolamine 유발 건망증 마우스 모델에서 육미지황탕(六味地黃湯)의 기억력 개선 및 항산화 효과

서영민; 한다영; 김상호; 정대규

doi:10.7231/jon.2018.29.4.207

Scopolamine 유발 건망증 마우스 모델에서 육미지황탕(六味地黃湯)의 기억력 개선 및 항산화 효과
Anti-amnesic Effect and Antioxidant Defense Systems of Yukmijihwang-tang on Scopolamine-induced Memory Impairment in Mice 원문보기

동의신경정신과학회지 = Journal of oriental neuropsychiatry, v.29 no.4, 2018년, pp.207 - 221

서영민 (대구한의대학교 한의과대학 신경정신과교실) , 한다영 (대구한의대학교 한의과대학 신경정신과교실) , 김상호 (대구한의대학교 한의과대학 신경정신과교실) , 정대규 (대구한의대학교 한의과대학 신경정신과교실)

Abstract ▼ AI-Helper

Objectives: The objective of this study was to observe the anti-amnesic effects of Yukmijihwang-tang (YMJHT), on the scopolamine (Sco)-induced memory impairment in C57BL/6 mice through its favorable acetylcholine (ACh). Also, to observe acetylcholinesterase (AChE) activity, Choline acetyltransferase (ChAT) mRNA expressions, and antioxidant effect. Methods: Six groups, with a total of 20 normal and 100 Sco treated mice were selected based on their body weights after 1 week of acclimatization, were used in this study as follows. Half of the mice in each group were used for passive avoidance task tests and hippocampus ACh content, AChE activity and ChAT mRNA expression measurement, and the remaining half in each group used for Morris water maze test and measurement of cerebral antioxidant defense system. Results: Amnesia due to AChE activations and destroyed cerebral cortex antioxidant defense systems were markedly and dose-dependently inhibited after 28 days of continuous oral pre-treatment with YMJHT 400, 200 and 100 mg/kg, respectively. The overall effects of YMJHT 400 mg/kg were similar to those of tacrine 10 mg/kg. Conclusions: Based on the results, it was established that oral administration of YMJHT favorably alleviates Sco-induced memory impairment, through preservation of ACh, mediated by up-regulation of ChAT mRNA expressions and related AChE inhibition and augmentation of cerebral antioxidant defense system, at least in a condition of this experiment. The overall effects of YMJHT 400 mg/kg were similar to those of tacrine 10 mg/kg.

주제어

표/그림 (11)

그림 Fig. 1. Experimental Design Used in This Study.
표 Table 1. Composition of YMJHT Used in This Study
표 Table 2. The Specific Sequence for Each Primer
그림 Fig. 2. Body Weight Changes in Normal Mice and Sco-induced Amnesia Mice.
표 Table 3. Changes on the Body Weight Gains in Normal Mice and Scopolamine-Induced Amnesia Mice
그림 Fig. 3. Changes on the Step-through Latency Times of Passive Avoidance Task Test in Normal Mice and Sco-induced Amnesia Mice.
그림 Fig. 4. Changes on the Escape Latency Times of Morris Water Maze Test in Normal Mice and Sco-induced Amnesia Mice.
그림 Fig. 5. Changes on the Hippocampus ACh Contents in Normal Mice and Sco-induced Amnesia Mice.
그림 Fig. 6. Changes on the Hippocampus AChE Activities in Normal Mice and Sco-induced Amnesia Mice.
그림 Fig. 7. Changes on the Hippocampus ChAT mRNA Expressions in Normal Mice and Sco-induced Amnesia Mice.
표 Table 4. Cerebral Cortex Antioxidant Defense Systems in Normal Mice and Scopolamine-induced Amnesia Mice

질의응답

핵심어	질문	논문에서 추출한 답변
	치매의 특징은 무엇인가	치매는 대표적인 노인성 질환의 하나이며, 알츠하이머병은 치매의 가장 흔한 원인 질환으로, 기억력 저하와 함께 다양한 신경 정신적 장애를 동반하며, 결국 움직일 수 없는 불능상태를 초래 한다1). 알츠하이머 환자에게서 뇌조직의 Acetylcoline (ACh) 감소는 치매의 중요한 병리적 소견이다2) .
	알츠하이머 치료제에는 어떤 것들이 있는가	현재까지 공식적으로 FDA로부터 허가를 받은 알츠하이머 치료제는 AChE를 억제하는 기전을 갖는 치료제가 대부분이다4) . Tacrine, donepezil, rivastigmine, galantamine 등이 대표적인 AChE 저해제이며, 그 중 Tacrine은 AChE 저해제로서는 최초로 FDA 승인을 받은 약물이지만, ALT 수치를 상승 시키는 등의 부작용으로5) 사용이 금지되었다. 그 외의 AChE 저해제들도 구역, 구토, 식욕감소, 장운동의 이상증가 등 다양한 부작용으로 그 사용이 제한되고 있는 실정이다.
	알츠하이머 환자에게서 치매의 중요한 병리적 소견은 무엇인가	치매는 대표적인 노인성 질환의 하나이며, 알츠하이머병은 치매의 가장 흔한 원인 질환으로, 기억력 저하와 함께 다양한 신경 정신적 장애를 동반하며, 결국 움직일 수 없는 불능상태를 초래 한다1). 알츠하이머 환자에게서 뇌조직의 Acetylcoline (ACh) 감소는 치매의 중요한 병리적 소견이다2) . ACh는 AChE에 의해 분해가 되며, AChE의 작용이 지속되면 ACh의 감소로 인해 기억력 저하가 발생 한다3).

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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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