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NTIS 바로가기Journal of embryo transfer = 한국수정란이식학회지, v.33 no.3, 2018년, pp.119 - 127
류지은 (한경대학교 미래융합기술대학원 동물생명공학과) , 윤종택 (한경대학교 미래융합기술대학원 동물생명공학과)
The purpose of this study is to examined the electrofusion and activation conditions for the production of porcine somatic cell nuclear transfer (SCNT) embryos. In this study, immature oocytes were cultured in TCM-199 with and without hormones for 22 hours. Skin fibroblasts cells of porcine were tra...
핵심어 | 질문 | 논문에서 추출한 답변 |
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복제 돼지 생산의 효율은 어느 정도인가? | 특히, SCNT에 의한 복제 돼지 생산은 면역이 억제된 조직과 장기를 생산하는 이종이식연구에 활용할 수 있기 때문에 특별한 중요성을 가지고 있다(Prather, 2000; Lai와 Prather, 2003). 그러나 돼지 복제 효율은 1-5%로 다른 가축에 비해 매우 낮다(Walker 등, 2002;Lai와 Prather, 2003). 이는 탈핵, 융합, 활성화 등과 같은 기술적인 요인들(Yang 등, 2003)과 비정상적 메틸화(Park 등, 2001; Jeong등, 2009), 유전자 발현의 문제(Park 등, 2010; Ko 등, 2011; Park 등, 2011)로 인한 것으로 알려져 있다. | |
체세포 핵이식은 어디에 유용한 기술인가? | 체세포 핵이식(SCNT: Somatic cell nuclear transfer)은 형질 전환 동물의 생산과 치료용 복제 동물을 생산하는데 유용한 기술이다. 특히, SCNT에 의한 복제 돼지 생산은 면역이 억제된 조직과 장기를 생산하는 이종이식연구에 활용할 수 있기 때문에 특별한 중요성을 가지고 있다(Prather, 2000; Lai와 Prather, 2003). | |
SCNT에 의한 복제 돼지 생산이 특별히 중요한 이유는? | 체세포 핵이식(SCNT: Somatic cell nuclear transfer)은 형질 전환 동물의 생산과 치료용 복제 동물을 생산하는데 유용한 기술이다. 특히, SCNT에 의한 복제 돼지 생산은 면역이 억제된 조직과 장기를 생산하는 이종이식연구에 활용할 수 있기 때문에 특별한 중요성을 가지고 있다(Prather, 2000; Lai와 Prather, 2003). 그러나 돼지 복제 효율은 1-5%로 다른 가축에 비해 매우 낮다(Walker 등, 2002;Lai와 Prather, 2003). |
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