Isolation of Lactobacillus plantarum subsp. plantarum Producing C30 Carotenoid 4,4'-Diaponeurosporene and the Assessment of Its Antioxidant Activity원문보기
Kim, Mibang
(Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University)
,
Seo, Dong-Ho
(Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University)
,
Park, Young-Seo
(Department of Food Science and Biotechnology, Gachon University)
,
Cha, In-Tae
(Microorganism Resources Division, National Institute of Biological Resources)
,
Seo, Myung-Ji
(Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University)
Carotenoids are organic pigments with antioxidant properties and are widespread in nature. Here, we isolated five microbes, each forming yellow-colored colonies and harboring C30 carotenoid biosynthetic genes (crtM and crtN). Thereafter, Lactobacillus plantarum subsp. plantarum KCCP11226, which show...
Carotenoids are organic pigments with antioxidant properties and are widespread in nature. Here, we isolated five microbes, each forming yellow-colored colonies and harboring C30 carotenoid biosynthetic genes (crtM and crtN). Thereafter, Lactobacillus plantarum subsp. plantarum KCCP11226, which showed the highest carotenoid production, was finally selected and the produced pigment was identified as C30 carotenoid 4,4'-diaponeurosporene. This strain exhibited the highest survival rate under oxidative stress and its carotenoid production was also enhanced after exposure to 7 mM H2O2. Moreover, it showed the highest ability to scavenge DPPH free radical. Our results suggested that L. plantarum subsp. plantarum KCCP11226, which produces 4,4'-diaponeurosporene as a natural antioxidant, may be a functional probiotic.
Carotenoids are organic pigments with antioxidant properties and are widespread in nature. Here, we isolated five microbes, each forming yellow-colored colonies and harboring C30 carotenoid biosynthetic genes (crtM and crtN). Thereafter, Lactobacillus plantarum subsp. plantarum KCCP11226, which showed the highest carotenoid production, was finally selected and the produced pigment was identified as C30 carotenoid 4,4'-diaponeurosporene. This strain exhibited the highest survival rate under oxidative stress and its carotenoid production was also enhanced after exposure to 7 mM H2O2. Moreover, it showed the highest ability to scavenge DPPH free radical. Our results suggested that L. plantarum subsp. plantarum KCCP11226, which produces 4,4'-diaponeurosporene as a natural antioxidant, may be a functional probiotic.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
Separation of the yellow pigments was performed using thin-layer chromatography (TLC) with silica gel (Merck, Darmstadt, Germany) and a mixture of petroleum ether and acetone (9:1, v/v) in a sealed glass chamber. After scraping off the pigment and eluting with petroleum ether to recover the pigment, the absorbance spectrum of the purified pigment was calculated using an ultraviolet-visible (UV-VIS) spectrophotometer (Shimadzu, Japan). The carotenoid pigment level was calculated by dividing the absorbance at 470 nm (A470) by the optical density at 600 nm (OD600).
대상 데이터
5:4; v/v/v). A UV-visible photodiode array detector was used, and detection was carried out at 470 nm.
데이터처리
Significant means were obtained after analysis of variance (ANOVA) analysis with Tukey-Kramer multiple comparison tests (p < 0.01).
Significant means were obtained by ANOVA with Bonferroni’s multiple comparison tests (p < 0.05).
Significant means were obtained by ANOVA with Tukey-Kramer multiple comparison tests (p < 0.01).
참고문헌 (38)
Armstrong GA. 1997. Genetics of eubacterial carotenoid biosynthesis: a colourful tale. Annu. Rev. Microbiol. 51: 629-659.
Jaswir I, Noviendri D, Hasrini RF, Octavianti F. 2011. Carotenoids: sources, medicinal properties and their application in food and nutraceutical industry. J. Med. Plants Res. 5: 7119-7131.
Ducrey Sanpietro LM, Kula MR. 1998. Studies of astaxanthin biosynthesis in Xanthophyllomyces dendrorhous (Phaffia rhodozyma). Effect of inhibitors and low temperature. Yeast 14: 1007-1016.
Del Campo JA, Moreno J, Rodriguez H, Angeles Vargas M, Rivas Joaquin, Guerrero MG. 2000. Carotenoid content of chlorophycean microalgae_factors determining lutein accumulation in Muriellopsis sp. (Chlorophyta). J. Biotechnol. 76: 51-59.
Li S, Zhao Y, Zhang L, Zhang X, Huang L, Li D, et al. 2012. Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods. Food Chem. 135: 1914-1919.
Serrano LM, Molenaar D, Wels M, Teusink B, Bron PA, de Vos WM, et al. 2007. Thioredoxin reductase is a key factor in the oxidative stress response of Lactobacillus plantarum WCFS1. Microb. Cell Fact. 6: 29.
Hagi T, Kobayashi M, Kawanoto S, Shima J, Nomura M. 2013. Expression of novel carotenoid biosynthesis genes from Enterococcus gilvus improves the multistress tolerance of Lactococcus lactis. J. Appl. Microbiol. 114: 1763-1771.
Turpin W, Renaud C, Avallone S, Hammoumi A, Guyot JP, Humblot C. 2016. PCR of crtNM combined with analytical biochemistry: an efficient way to identify carotenoid producing lactic acid bacteria. Syst. Appl. Microbiol. 39: 115-121.
Ben-Amotz A, Avron M. 1983. On the factors which determine massive ${\beta}$ -carotene accumulation in the halotolerant alga Dunaliella bardawil. Plant. Physiol. 72: 593-597.
Hagi T, Kobayashi M, Nomura M. 2014. Aerobic condition increases carotenoid production associated with oxidative stress tolerance in Enterococcus gilvus. FEMS Microbiol. Lett. 350: 223-230.
Kimoto-Nira H, Kobayashi M, Nomura M, Sasaki K, Suzuki C. 2009. Bile resistance in Lactococcus lactis strains varies with cellular fatty acid composition: analysis by using different growth media. Int. J. Food Microbiol. 131: 183-188.
Neviani E, Carminati D, Veaux M, Hermier J, Giraffa G. 1991. Characterization of Lactobacillus helveticus strains resistant to lysozyme. Lait 71: 65-73.
Hagi T, Kobayashi M, Nomura M. 2014. Aerobic conditions increase isoprenoid biosynthesis pathway gene expression levels for carotenoid production in Enterococcus gilvus. FEMS Microbiol. Lett. 362: 223-230.
Lim HS, Cha I, Roh SW, Shin H, Seo M. 2017. Enhanced producion of gamma-aminobutyric acid by optimizing culture conditions of Lactobacillus brevis HYE1 isolated from kimchi, a korean fermented food. J. Microbiol. Biotechnol. 27: 450-459.
Wieland B, Feil C, Gloria-Maercker E, Thumm G, Lechner M, Bravo JM, et al. 1994. Genetic and biochemical analyses of the biosynthesis of the yellow carotenoid 4,4'-diaponeurosporene of Staphylococcus aureus. J. Biotechnol. 176: 7719-7726.
Kobayashi M, Kakizono T, Nagai S. 1993. Enhanced carotenoid biosynthesis by oxidative stress in acetate-induced cyst cells of green unicellular alga, Haematococcus pluvialis. Appl. Environ. Microbiol. 59: 867-873.
Clauditz A, Resch A, Wieland KP, Peschel A, Gotz F. 2006. Staphyloxanthin plays a role in the fitness of Staphylococcus aureus and its ability to cope with oxidative stress. Infect. Immun. 74: 4950-4953.
Shimamura S, Abe F, Ishibashi N, Miyakawa H, Yaeshima T, Araya T, et al. 1992. Relationship between oxygen sensitivity and oxygen metabolism of Bifidobacterium species. J. Dairy Sci. 75: 3296-3306.
Marova I, Carnecka M, Halienova A, Breierova E, Koci R. 2010. Production of carotenoid-/ergosterol-supplemented biomass by red yeast Rhodotorula glutinis grown under external stress. Food Technol. Biotechnol. 48: 56-61.
Jeong JC, Lee IY, Kim SW, Park YH. 1999. Stimulation of ${\beta}$ -carotene synthesis by hydrogen peroxide in Blakeslea trispora. Biotechnol. Lett. 21: 683-686.
Jeong S, Kang CK, Choi YJ. 2018. Metabolic engineering of Deinococcus radiodurans for the production of phytoene. J. Microbiol. Biotechnol. 28: 1691-1699.
Yatsunami R, Ando A, Yang Y, Takaichi S, Kohno M, Matsumura Y, et al. 2014. Identification of carotenoids from the extremely halophilic archaeon Haloarcula japonica. Front. Microbiol. 5: 100.
Manimala MRA, Murugesan R. 2014. In vitro antioxidant and antimicrobial activity of carotenoid pigment extracted from Sporobolomyces sp. Isolated from natural source. J. Appl. Nat. Sci. 6: 649-653.
Zhang L, Liu C, Li D, Zhao Y, Zhang X, Zeng X, et al. 2013. Antioxidnat activity of an exopolysaccharide isolated from Lactobacillus plantarum C88. Int. J. Biol. Macromol. 54: 270-275.
Suzuki Y, Kosaka M, Shindo K, Kawasumi T, Kimoto-Nira H, Suzuki C. 2013. Identification of antioxidants produced by Lactobacillus plantaum. Biosci. Biotechnol. Biochem. 77: 1299- 1302.
Steiger S, Perez-Fons L, Fraser PD, Sandmann G. 2012. Biosynthesis of a novel $C_{30}$ carotenoid in Bacillus firmus isolates. J. Appl. Microbiol. 113: 888-895.
Wu Y, Ma Y, Li L, Yang X. 2018. Preparation and antioxidant activities in vitro of a designed antioxidant peptide from pinctada fucata by recombinant Escherichia coli. J. Microbiol. Biotechnol. 28: 1-11.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.