[논문]High-resolution 1H NMR Spectroscopy of Green and Black Teas

Jeong, Ji-Ho; Jang, Hyun-Jun; Kim, Yongae

doi:10.5012/jkcs.2019.63.2.78

High-resolution 1H NMR Spectroscopy of Green and Black Teas 원문보기

대한화학회지 = Journal of the Korean Chemical Society, v.63 no.2, 2019년, pp.78 - 84

Jeong, Ji-Ho (Department of Chemistry, Hankuk University of Foreign Studies) , Jang, Hyun-Jun (Department of Chemistry, Hankuk University of Foreign Studies) , Kim, Yongae (Department of Chemistry, Hankuk University of Foreign Studies)

Abstract ▼ AI-Helper

High-resolution $^1H$ NMR spectroscopic technique has been widely used as one of the most powerful analytical tools in food chemistry as well as to define molecular structure. The $^1H$ NMR spectra-based metabolomics has focused on classification and chemometric analysis of complex mixtures. The principal component analysis (PCA), an unsupervised clustering method and used to reduce the dimensionality of multivariate data, facilitates direct peak quantitation and pattern recognition. Using a combination of these techniques, the various green teas and black teas brewed were investigated via metabolite profiling. These teas were characterized based on the leaf size and country of cultivation, respectively.

주제어

표/그림 (6)

그림 Figure 1. ¹H NMR spectra of green tea brewed 5 min (A), brewed 10 min (B), black tea brewed 5 min (C) and brewed 10 min (D). The detailed ¹H NMR peak assignments of compounds from green tea and black tea are shown in Table 1.
표 Table 1. ¹H NMR chemical shift data for green tea
그림 Figure 2. ¹H NMR spectra of green tea of (A) Woojeon, (B) Jaksul and (C) Daejak. They were classified according to cultivated time.
그림 Figure 3. PCA score plot for the ¹H NMR spectra of green tea (circles) and black tea (triangles). The variation of green tea is wider than black tea but the two clusters are discriminated clearly.
그림 Figure 4. PCA on the ¹H NMR spectra of green teas with different size of the leaves. Circles indicate that leaves were cultivated end of April to early May. Triangles and squares indicate leaves were cultivated early May to end of May and June to July respectively.
그림 Figure 5. PCA score plot on the ¹H NMR spectra of black teas cultivated at different countries. Circles indicate that leaves from China. Triangles and squares indicate leaves from India and Sri Lanka respectively. Three clusters are separated clearly with PC1 and PC2.

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제안 방법

65 ppm suggested possible delineation between the 3 groups. In order to distinguish the metabolite profiles of brewed black tea leaves cultivated in three different countries (China, India and Sri Lanka), 11 different black tea samples were analyzed using ¹H NMR spectroscopy combined with PCA. It was used to identify the different metabolites in three groups and showed significant correlation between the components and the effect of cultivation in different countries.
In this paper, the various metabolites of green tea and black tea were identified and the quantitative changes in metabolites with brew times were observed using high resolution ¹H NMR coupled with multivariate statistical analysis. In addition, the qualities of green tea and black tea were classified according to growth environments.
In order to distinguish the metabolite profiles of brewed black tea leaves cultivated in three different countries (China, India and Sri Lanka), 11 different black tea samples were analyzed using ¹H NMR spectroscopy combined with PCA. It was used to identify the different metabolites in three groups and showed significant correlation between the components and the effect of cultivation in different countries.

대상 데이터

A set of 10 commercial green teas and 16 commercial black tea samples were analyzed. All kinds of dried tea samples of 500 mg were added to 50 mL de-ionized hot water at 85 °C for 5 to 10 min and 2 aliquots of 500 µL each of brewed tea were transferred to the 1.

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