[논문]U937 세포에서 육계와 온열 병행 치료가 세포증식 억제와 세포사멸 유도에 미치는 연구

안채령; 박선향; 김홍준; 정민정; 백승호

doi:10.14374/hfs.2019.27.1.45

U937 세포에서 육계와 온열 병행 치료가 세포증식 억제와 세포사멸 유도에 미치는 연구
Treatment of Cinnamomi Cortex combined with hyperthermia synergistically suppressed proliferation and induced apoptosis in U937 cell line. 원문보기

大韓韓醫學方劑學會誌 = Herbal formula science, v.27 no.1, 2019년, pp.45 - 52

안채령 (우석대학교 식품생명공학과) , 박선향 (우석대학교 한의과대학 병리학교실) , 김홍준 (우석대학교 한의과대학 방제학교실) , 정민정 (우석대학교 한의과대학 한방소아과학교실) , 백승호 (우석대학교 한의과대학 병리학교실)

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Objectives : Hyperthermia is a widely used therapeutic tool for cancer therapy and a well-known inducer of apoptosis. Although the Cinnamomi cortex (CC) is a potent anticancer agent for several human carcinomas, it is less potent in the human U937 cell line. To explore any enhancing effects of CC with hyperthermia induced apoptosis, this study investigated the combined effects and apoptotic mechanisms of hyperthermia and CC in U937 cells. Methods : U937 cells were heat treated at $43^{\circ}C$ for 30 min with or without pre-treatment for 1h with CC and then incubated at $37^{\circ}C$ with 5% $CO_2$. Cell viability was analyzed by MTT assay and Trypan blue assay. Morphological changes reflecting apoptosis were visualized under microscope. Synergy effect of CC combined with hyperthermia were calculated by Compusyn software. The expression of proteins related to apoptosis and signaling pathways was determined by western blotting. Results : Hyperthermia with CC reduced cell viability and induced apoptosis. Combined hyperthermia and CC treatment markedly augmented apoptosis by upregulating proapoptotic proteins and suppressing antiapoptotic proteins, culminating in caspase-3 activation. Furthermore, the combined treatment, decreased the expression of in Bcl-2 family, cyclin D1, VEGF, MMP2 and MMP9 expression. Conclusion : This study provides compelling evidence that hyperthermia, in combination with CC, is a promising therapeutic strategy for enhancement of apoptosis and suggests a promising therapeutic approach for cancer.

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표/그림 (3)

그림 Fig. 1 Apoptosis induce by CC with hyperthermia in U937 cells.
그림 Fig. 2 Effect of CC with hyperthermia on the levels of caspase-3, Bcl-2 and Bcl-xL protein expression in the control group and treatment groups.
그림 Fig. 3 Expression of cyclin D1, VEGF, MMP-2, and MMP-9 in U937 cells by Western blots.

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All numeric values are represented as the mean ± SD. Statistical significance of the data compared with the untreated control was determined using the Student unpaired t-test. *p<0.

이론/모형

1 Apoptosis induce by CC with hyperthermia in U937 cells. (A and B) Percentage of cell viability was determined using MTT assay (A) and Trypan blue assay (B). Cell viability was decreased in the 43℃ treatment group compared with the 37℃ and 42℃ treatment groups in a dose-dependent manner for 48h (A) and 24h (B).

참고문헌 (16)

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