[논문]고지방식이로 유도된 비만 쥐의 골격근에서 유산소 운동 훈련 또는 레스베라트롤 투여가 미토콘드리아 생합성에 미치는 영향

김경일; 안상민; 박희근; 이왕록

doi:10.5352/jls.2019.29.8.837

고지방식이로 유도된 비만 쥐의 골격근에서 유산소 운동 훈련 또는 레스베라트롤 투여가 미토콘드리아 생합성에 미치는 영향
The Effect of Aerobic Exercise Training Versus Resveratrol Supplementation on Mitochondrial Biogenesis in Skeletal Muscle of High-fat Diet-induced Obese Mice 원문보기

생명과학회지 = Journal of life science, v.29 no.8, 2019년, pp.837 - 845

김경일 (충남대학교 스포츠과학과) , 안상민 (고려대학교 사회체육학과) , 박희근 (충남대학교 스포츠과학과) , 이왕록 (충남대학교 스포츠과학과)

초록
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본 연구에서는 고지방식이로 유발된 비만 쥐의 골격근에서 유산소 운동과 레스베라트롤 투여가 미토콘드리아 생합성에 미치는 영향을 조사하였다. 4주령 C57BL/6의 수컷 쥐를 이용하여, 일반 식이 그룹(NC, n=10), 고지방식이 그룹(HR, n=10), 레스베라트롤 투여와 고지방식이 그룹(HRe, n=10), 유산소 운동 그룹(HE, n=10)으로 분류하였다. 유산소 운동은 16주 동안 40~60 min/day 동안 10-14m/min, 0% grade의 강도로 주당 4회 트레드밀 운동을 실시하였고, 레스베라트롤은 16주 동안 1일 1회, 주당 4회 체중 당 25 mg/kg을 투여하였다. COX-IV mRNA 발현은 NC와 HC 그룹 간에 유의한 차이가 있었으며(p<0.05), HE 그룹의 SIRT-3, $PGC-1{\alpha}$ 및 COX-IV mRNA 발현은 HC 및 HRe 그룹에 비해 유의하게 증가하였다(p<0.05). 또한, 오직 HE 그룹의 $PGC-1{\alpha}$ 및 COX-IV mRNA의 발현만이 HC 그룹에 비해 유의하게 증가하였다(p<0.05). 이상의 결과를 종합해보면, 고지방식이로 유발된 비만 쥐는 골격근에서 미토콘드리아 생합성 유전자 발현에 영향을 나타내지 않는 것으로 보인다. 하지만, 유산소 운동 훈련은 고지방식이로 유발된 비만 쥐의 골격근에서 미토콘드리아 생합성 유전자 발현을 증가시키는 것으로 나타났다. 이러한 연구 결과는 레스베라트롤 투여가 아닌 유산소 운동이 고지방식이로 유도된 쥐의 골격근에서 미토콘드리아 생합성에 긍정적인 영향을 미친다는 것을 시사한다.

Abstract ▼ AI-Helper

The purpose of this study was to analyze the effects of aerobic exercise and resveratrol supplementation on mitochondrial biogenesis in skeletal muscle of high-fat diet-induced obese mice. In this study, 4-wk-old C57BL/6 male mice were divided into four groups, with 10 animals in each group: a normal diet group (NC), high-fat diet group (HC), high-fat diet group with resveratrol supplementation (HRe), and high-fat diet GROUP with exercise (HE). Aerobic exercise was performed on a treadmill for 40~60 min/d at 10~14 m/min, 0% grade, 4 d/wk for 16 wk. Resveratrol (25 mg/kg bodyweight) was administrated once a day, 4 d/wk for 16 wk. There was a significance difference in COX-IV mRNA expression in the NC group versus that in the HC group (p<0.05). The expression of the SIRT-3, PGC-1a, and COX-IV mRNA genes in the HE group increased significantly as compared with the expression of these genes in the HC and HRe groups (p<0.05). These results indicated that high- fat diet-induced obesity did not affect mitochondria biogenesis gene expression in skeletal muscle. In contrast, aerobic exercise training increased the expression of mitochondria biogenesis gene expression in skeletal muscle in high-fat diet-induced obese mice. These findings suggested that aerobic exercise but not resveratrol supplementation had a positive effect on mitochondrial biogenesis in skeletal muscle in high-fat diet-induced obese mice.

주제어

표/그림 (7)

표 Table 1. Formulas of rodent feed
표 Table 2. Exercise protocol
표 Table 3. Primer sequences used for Real-time PCR
그림 Fig. 1. The comparison of body and muscle weight among groups.
그림 Fig. 2. The comparison of food and calorie intake among groups.
그림 Fig. 3. The comparison of SIRT-1 and SIRT-3 mRNA among groups.
그림 Fig. 4. The comparison of PGC-1α and COX-IV mRNA among groups.

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제안 방법

Exercise training was performed on a motor treadmill at moderate intensity for 16 weeks, 4 days/week for 40-60 min/day. The exercise was performed at a speed of 10 m/min for 1-2 weeks, 12 m/min for 3-5 weeks and 14 m/min for 6-16 weeks.
The classification of groups was classified into total four groups such as normal diet group (NC, n=10), high fat diet group (HC), high fat diet group with resveratrol supplementation (HRe, n=10) and high fat diet with exercise group (HE, n=10) and then resveratrol supplementation and exercise were applied for 16 weeks. All experiments were approved by the Animal Care and Use Committee at the Chungnam National University (CNU-00494).
Exercise training was performed on a motor treadmill at moderate intensity for 16 weeks, 4 days/week for 40-60 min/day. The exercise was performed at a speed of 10 m/min for 1-2 weeks, 12 m/min for 3-5 weeks and 14 m/min for 6-16 weeks. This exercise intensity be selected to 60-76% of maximal oxygen uptake [51] because it was known that moderate endurance exercise training affected mitochondrial biogenesis [21].
Therefore, the purpose of this study was to analyze the effects of either aerobic exercise or resveratrol supplementation on mitochondrial biogenesis (SIRT-1, SIRT-3, PGC-1α, COX-IV mRNA) in skeletal muscle of high fat diet-induced obesity mice.

대상 데이터

4-week-old male C57BL/6 (Central Experiment Animal, Korea, n=40) mice were housed in cages (5 mice per cage) in a standard experimental laboratory, at a temperature of 22±2℃, with 50±5% humidity.

데이터처리

Statistical analysis of the data was performed by SPSS Version 22.0 using one-way ANOVA with LSD post-hoc tests. Statistical significance was defined as α=.

성능/효과

4-week-old male C57BL/6 (Central Experiment Animal, Korea, n=40) mice were housed in cages (5 mice per cage) in a standard experimental laboratory, at a temperature of 22±2℃, with 50±5% humidity. After a one-week acclimatization period, the mice were fed either a high fat diet (45% of energy from fat, Orient Bio Inc., # D12451) or a normal diet (10% of energy from fat, Orient Bio Inc., # D12451) ad libitum for 16 weeks (Table 1).
It means that the effect of resveratrol supplementation was not enough to reduce body weight and to activate mitochondrial biogenesis whereas aerobic exercise training had a positive effect to reduce body weight and improve mitochondria biogenesis gene activation. In conclusion, aerobic exercise training was more effective than resveratrol supplementation to increase mitochondrial biogenesis markers in high fat diet induced obese mice. Furthermore, aerobic exercise has a kind of medicine to ameliorate high fat diet-induced metabolic complication.
Resveratrol supplementation has been reported to induce transcription of nuclear-encoded mitochondrial sub-signaling substances such as COX-IV and to improve mitochondrial function as well as mitochondrial respiration [50]. In this study, high-fat diet group with resveratrol treatment (HRe) showed no significant difference in the expression level of skeletal muscle COX-IV mRNA compared to the high-fat diet group (HC). Although these results are in contrast to the results of Scarpulla et al.
Therefore, high-fat diet may have a negative effect on SIRT-3 activation of skeletal muscle in high fat diet-induced obese mice. In this study, the expression of skeletal muscle SIRT-3 mRNA of high-fat diet and exercise group (HE) was a significantly increased compared to that of high-fat diet group (HC). These results are consistent with the results of previous studies [15, 20, 27, 45].
In this study, there was no significance among NC, HCh and HRe groups, it means that high fat diet-induced obese did not affect to the gene expression of SIRT-3. However, there was a tendency that high fat diet-induced obese made the gene expression of SIRT-3 reduced.
[11] was reported that resveratrol induces activation of SIRT-3 protein and has been shown to have a beneficial effect on health by activating the SIRT-3. In this study, there was no significant difference of SIRT-3 mRNA expression level in the normal diet group (NC), the high-fat diet group (HC) and high-fat diet group with resveratrol treatment (HRe) of the skeletal muscle. Those result is contrast to the study by Das et al.
In this study, there was no significant difference of expression of skeletal muscle PGC-1α mRNA among normal diet group (NC), high-fat diet group (HC) and high-fat diet with resveratrol (HRe).

후속연구

Further, as the effect of resveratrol was compared to the exercise intervention, the effect of resveratrol might be reduced in statistics. Therefore, further research will be needed.
It seems that exercise training not resveratrol supplementation has a positive effect to increase skeletal muscle weight. Thus, further study is needed to understand better with a various dosage and duration of resveratrol supplementation on body weight and muscle mass.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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