[논문]Antimalarial Activity and Phytochemical Profile of Ethanolic and Aqueous Extracts of Bidara Laut (Strychnos ligustrina Blum) Wood

MANURUNG, Harisyah; SARI, Rita Kartika; SYAFII, Wasrin; CAHYANINGSIH, Umi; EKASARI, Wiwied

doi:10.5658/wood.2019.47.5.587

Antimalarial Activity and Phytochemical Profile of Ethanolic and Aqueous Extracts of Bidara Laut (Strychnos ligustrina Blum) Wood 원문보기

목재공학 = Journal of the Korean wood science and technology, v.47 no.5, 2019년, pp.587 - 596

MANURUNG, Harisyah (Department of Forest Products, Faculty of Forestry, IPB University (Bogor Agricultural University)) , SARI, Rita Kartika (Department of Forest Products, Faculty of Forestry, IPB University (Bogor Agricultural University)) , SYAFII, Wasrin (Department of Forest Products, Faculty of Forestry, IPB University (Bogor Agricultural University)) , CAHYANINGSIH, Umi (Department of Animal Health and Veterinary Medicine, Faculty of Veterinary, IPB University (Bogor Agricultural University)) , EKASARI, Wiwied (Department of Pharmacognosy and Phytochemical, Faculty of Pharmacy, Airlangga University)

Abstract ▼ AI-Helper

This study aimed to determine the antimalarial effect of the Strychnos ligustrina (SLW) wood extracts and to analyze its phytochemicals. The SLW powder samples were macerated with 100% ethanol (E100), 75% ethanol (E75), 50% ethanol (E50), 25% ethanol (E25), and aqueous (A100). The extracts were analyzed by LCMS/MS, and its in-vitro antimalarial activity was tested with Plasmodium falciparum. The results showed that the extract yields of E100, E75, E50, E25, and A100 were 4.3, 5.2, 5.3, 4.7, and 3.6%, respectively. The antimalarial activities of the A100, E25, E50, and E75 extracts were classified as active with $IC_{50}$ values of 38.6, 42.6, 42.9, and $43.7{\mu}g/mL$, respectively. But, the antimalarial activity of the E100 extract was classified as slightly active with $IC_{50}$ values of $87.4{\mu}g/mL$. The dominant compounds contained in the extracts of A100, E25, E50, E75, and E100 was the alkaloid compound, namely brucine with relative concentrations of 24.96, 24.55, 21.33, 11.79, and 11.62%, respectively.

주제어

표/그림 (5)

그림 Fig. 1. The relationship Graph of the concentrations of the A100, E25, E50, E75, and E100 extracts of S. ligustrina wood with percent of the P. falciparum parasite inhibitors and regression equation.
그림 Fig. 2. The IC₅₀ value of the A100, E25, E50, E75, and E100 extracts of S. ligustrina wood.
표 Table 1. The yield of S. ligustrina wood extracted by maceration method
표 Table 2. Results of qualitative phytochemical analysis of S. ligustrina wood extract
표 Table 3. The results of LCMS / MS analysis of S. ligustrina wood extract

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제안 방법

The stages were incomplete medium preparation, serum preparation, complete medium preparation, erythrocytes preparation, P. falciparum parasites breeding, maintenance of P. falciparum parasites, synchronization, in vitro antimalarial activity in various concentrations (0.01, 0.1, 1, 10, and 100 µg/mL), harvesting, and data analysis.
ligustrina wood extract using ethanol and aqueous and a mixture of both at various concentrations is crucial to produce a safe and active standardized extract. This study aimed to determine the yield extract from S. ligustrina wood extract using 100% ethanol (E100), 75% ethanol (E75), 50% ethanol (E50), 25% ethanol (E25), and aqueous (A100); determine the in vitro antimalarial activity based on the inhibition of P. falciparum; and analyze phytochemical extracts qualitatively and quantitatively with Liquid Chromatography-tandem Mass Spectroscopy (LCMS/MS).

대상 데이터

5%; N-2-hydroxyl ethyl piperazine-N-2-ethane sulfonic acid (HEPES); gentamicin sulfate; serum and red blood cells (RBC); and citric phosphate dextrose anticoagulant (CPD). The tools used were extractor jars, rotavators, ovens, incubators, Millipore membranes, centrifuge tubes, UV lamps, Laminar Air Flow chambers, desiccators, vortex devices, and light microscopes.

데이터처리

The yield of each extract was compared using Duncan's test for further analysis.

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