[논문]유묘기 양배추류에서 메틸자스모네이트에 의한 글루코시놀레이트 함량 변화 및 전사체 발현 분석

이정여; 민성란; 정재은; 김혜란

doi:10.5010/jpb.2019.46.3.189

유묘기 양배추류에서 메틸자스모네이트에 의한 글루코시놀레이트 함량 변화 및 전사체 발현 분석
Effect of methyl jasmonate on the glucosinolate contents and whole genome expression in Brassica oleracea 원문보기

Journal of plant biotechnology = 식물생명공학회지, v.46 no.3, 2019년, pp.189 - 204

이정여 (한국생명공학연구원) , 민성란 (한국생명공학연구원) , 정재은 (한국생명공학연구원) , 김혜란 (한국생명공학연구원)

초록
AI-Helper

본 연구의 목적은 유묘기 TO1000DH3와 Early big에서 MeJA 처리에 의해 글루코시놀레이트 함량 변화 및 유전자의 발현 변화를 분석하기 위하여 수행되었다. $200{\mu}M$ 농도의 MeJA를 처리하여 글루코시놀레이트 함량을 분석한 결과, 글루코시놀레이트 총 함량이 처리 전보다 TO1000DH3에서 1.3~1.5배, Early big에서 1.3 ~ 3.8배 증가하였다. 알리패틱 글루코시놀레이트인 progoitrin과 gluconapin은 TO1000DH3에서만 검출되었으며, neoglucobrassicin 성분의 함량 변화가 MeJA 처리 48시간 후 TO1000DH3와 Early big에서 가장 크게 증가되었다. 전사체 분석을 통해 TO1000DH3에서는 stress나 defense 반응에 관여하거나, 생장과 관련된 전사체가 특이적으로 발현하고, Early big에서는 nucleoside 또는 ATP 생합성 관련 전사체가 특이적으로 발현하는 것을 알 수 있었다. MeJA를 처리함에 따라 발현이 2배 이상 변한 전사체를 TO1000DH3에서 12,020개, Early big에서 13,510개를 선발하여 GO 분석한 결과 stimulus, chemical에 반응하는 전사체의 발현이 공통적으로 증가하였고, single-organism 및 ribosome 합성 관련 전사체의 발현이 공통적으로 감소하였다. 특히 glucobrassicin, neoglucobrassicin 함량과 연관되어 발현이 증가한 인돌릭 글루코시놀레이트 생합성 관련 전사체의 발현이 모두 증가하였다 (MYB34 (Bo7g098110), IGMT2 (Bo8g070650), CYP81D1 (Bo6g056440), CYP81D4 (Bo7g118500), CYP81F4 (Bo1g004730, Bo01007s020), CYP81G1 (Bo4g154660), CYP83B1 (Bo8g024390) 및 CYP91A2 (Bo1g003710)). 글루코시놀레이트 생합성 경로 관련 유전자를 대표하는 전사체 104개를 선발하여 발현 양상을 분석한 결과 transcription factor에 속하는 MYB28, MYB51의 발현은 MeJA 처리 전에 비해 처리 후 발현양이 감소하였지만, 대부분의 전사체의 발현은 MeJA 처리에 의해 증가하였다. MeJA 처리에 의해 AOP3 (Bo9g006220, Bo9g006240), TGG1 (Bo14804s010)는 TO1000DH3에서만 특이적으로 발현이 증가하였고, Dof1.1 (Bo5g008360), UGT74C1 (Bo4g177540), GSL-OH (Bo4g173560, Bo4g173550, Bo4g173530)는 Early big 특이적으로 발현이 증가하였다. MeJA 처리 전 두 계통에서 발현이 가장 높은 글루코시놀레이트 생합성 관련 유전자는 GSTU20이었고, MeJA 처리에 의해 12시간 후TO1000DH3에서 CYP79B2 (Bo7g118840), Early big에서는 CYP79B3 (Bo4g149550)의 발현이 가장 많이 증가하였다.

Abstract ▼ AI-Helper

In this study, we analyzed the changes in glucosinolate content and gene expression in TO1000DH3 and Early big seedling upon methyl jasmonate (MeJA) treatment. Analysis of glucosinolate contents after MeJA treatment at $200{\mu}M$ concentration showed that the total glucosinolate content increased by 1.3-1.5 fold in TO1000DH3 and 1.3-3.8 fold in Early big compared to those before treatment. Aliphatic glucosinolates, progoitrin and gluconapin, were detected only in TO1000DH3, and the changes in the content of neoglucobrassicin were the greatest at 48 hours after MeJA treatment in TO1000DH3 and Early big. The transcriptomic analysis showed that transcripts involved in stress or defense reactions, or those related to growth were specifically expressed in TO1000DH3, while transcripts related to nucleosides or ATP biosynthesis were specifically expressed in Early big. GO analysis on transcripts with more than two-fold change in expression upon MeJA treatment, corresponding to 12,020 transcripts in TO1000DH3 and 13,510 transcripts in Early big, showed that the expression of transcripts that react to stimulus and chemical increased in TO1000DH3 and Early big, while those related to single-organism and ribosome synthesis decreased. In particular, the expression increased for all transcripts related to indole glucosinolate biosynthesis, which is associated with increase in glucobrassicin and neoglucobrassicin contents. Upon MeJA treatment, the expression of AOP3 (Bo9g006220, Bo9g006240), TGG1 (Bo14804s010) increased only in TO1000DH3, while the expression of Dof1.1 (Bo5g008360), UGT74C1 (Bo4g177540), and GSL-OH (Bo4g173560, Bo4g173550, Bo4g173530) increased specifically in Early big.

주제어

참고문헌 (52)

Baenas N, Garcia-Viguera C, Moreno DA (2014) Biotic elicitors effectively increase the glucosinolates content in Brassicaceae sprouts. J Agric Food Chem 62:1881-1889

상세보기
Belser C, Istace B, Denis E, Dubarry M, Baurens FC, Falentin C, Genete M, Berrabah W, Chevre AM, Delourme R, Deniot G, Denoeud F, Duffe P, Engelen S, Lemainque A, Manzanares-Dauleux M, Martin G, Morice J, Noel B, Vekemans X, D'Hont A, Rousseau-Gueutin M, Barbe V, Cruaud C, Wincker P, Aury JM (2018) Chromosome-scale assemblies of plant genomes using nanopore long reads and optical maps. Nat Plants 4:879-887

상세보기
Bhandari SR, Jo JS, Lee JG (2015) Comparison of glucosinolate profiles in different tissues of nine Brassica crops. Molecules 20:15827-15841

상세보기
Cartea ME, Velasco P, Obregon S, Padilla G, Haro A (2008) Seasonal variation in glucosinolate content in Brassica oleracea crops grown in northwestern Spain. Phytochemistry 69:403-410

상세보기
Cheong JJ, Choi YD (2003) Methyl jasmonate as a vital substance in plants. Trends Genet 19:409-413

상세보기
Choi DW, Jung JD, Ha YI, Park HW, In DS, Chung HJ (2005) Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites. Plant Cell Rep 23:557-566

상세보기
Choi YH, Park KY, Lee SM, Yoo MA, Lee WH (1995) Inhibitory effect of the fresh juice of kale on the genotoxicity of aflatoxin B1. Korean J Genetic 17:183-190
Clarke DB (2010) Glucosinolates, structures and analysis in food. Anal Methods 2:310-325

상세보기
Dixon RA, Paiva NL (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085-1097

상세보기
Fahey JW, Zalcmann AT, Talalay P (2001) The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56:5-51

상세보기
Gao J, Yu X, Ma F, Li J (2014) RNA-seq analysis of transcriptome and glucosinolate metabolism in seeds and sprouts of broccoli (Brassica oleracea var. italic). PLoS One 9(2): e88804

상세보기
Goncalves ALM, Lemos M, Niero R, Andrade SF, Maistro EL (2012) Evaluation of the genotoxic and antigenotoxic potential of Brassica oleracea L. var. acephala D.C. in different cells of mice. J Ethnopharmacol 143:740-745

상세보기
Gutjahr C, Riemann M, Muller A, Duchting P, Weiler EW, Nick P (2005) Cholodny-Went revisited: a role for jasmonate in gravitropism of rice coleoptiles. Planta 222:575-585

상세보기
Hagen SF, Borge GIA, Solhaug KA, Bengtsson GB (2009) Effect of cold storage and harvest date on bioactive compounds in curly kale (Brassica oleracea L. var. acephala). Postharvest Biol Technol 51:36-42

상세보기
Halkier BA, Du L (1997) The biosynthesis of glucosinolates. Trends Plant Sci 2:425-431

상세보기
Higdon JV, Delage B, Williams DE, Dashwood RH (2007) Cruciferous vegetables and human cancer risk: epidemiologic evidence and mechanistic basis. Pharmacol Res 55: 224-236

상세보기
Holst B, Williamson G (2004) A critical review of the bioavailability of glucosinolates and related compounds. Nat Prod Rep 21:425-447

상세보기
Hwang ES, Hong EY, Kim GH (2012) Determination of bioactive compounds and anti-biocancer effect from extracts of Korean cabbage and cabbage. Korean J Food Nutr 25:259-265

원문보기 상세보기
Islam MM, Tani C, Watanabe-Sugimoto M (2009) Myrosinases, TGG1 and TGG2, redundantly function in ABA and MeJA signaling in Arabidopsis guard cells. Plant Cell Physiol 50:1171-1175

상세보기
Izzah NK, Lee J, Jayakodi M, Perumal S, Jin M, Park BS, Ahn K, Yang TJ (2014) Transcriptome sequencing of two parental lines of cabbage (Brassica oleracea L. var. capitata L.) and construction of an EST-based genetic map. BMC Genomics 15:149

상세보기
Keck AS, Finley JW (2004) Cruciferous vegetables: cancer protective mechanisms of glucosinolate hydrolysis products and selenium. Integr Cancer Ther 3:5-12

상세보기
Ketchum REB, Gibson DM, Croteau RB, Shuler ML (1999) The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol Bioeng 62:97-105

상세보기
Keum YS, Jeong WS, Kong ANT (2004) Chemoprevention by isothiocyanates and their underlying molecular signaling mechanisms. Mutat Res Fundam Mol Mech Mutagen 555:191-202

상세보기
Kim JA, Bae KH, Choi YE (2015) Effect of elicited by methyl jasmonate on the saponin contents of Codonopsis lanceolate. J Plant Biotechnol 42:265-270

원문보기 상세보기
Kim OT, Bang KH, Kim YC, Hyu DY, Kim MY (2009) Upregulation of ginsenoside and gene expression related to triterpene biosynthesis in ginseng hairy root cultures elicited by methyl jasmonate. Plant Cell Tissue Organ Cult 98:25-33

상세보기
Kim OT, Bang KH, Shin YS, Lee MJ, Jung SJ, Hyun DY, Kim YC, Seong NS, Cha SW (2007) Enhanced production of asiaticoside from hairy root cultures of Centella asiatica (L.) Urban elicited by methyl jasmonate. Plant Cell Rep 26:1941-1949

상세보기
Kim YS, Hahn EJ, Murthy HN, Paek KY (2004) Adventitious root growth and ginsenoside accumulation in Panax ginseng cultres as affected by methyl jasmonate. Biotechnol Lett 26:1619-1622

상세보기
Kim YS, Milner JA (2005) Targets for indole-3-carbinol in cancer prevention. J Nutr Biochem 16:65-73

상세보기
Kim YW, Jung HJ, Park JI, Hur Y, Nou IS (2014) Response of NBS encoding resistance genes linked to both heat and fungal stress in Brassica oleracea. Plant Physiol Biochem 86:130-136
Kowalsky SP, Lan TH, Feldmann KA, Paterson AH (1994) Comparative mapping of Arabidopsis thaliana and Brassica oleracea. G3 138:499-510
Kukurba KR, Montgomery SB (2015) RNA Sequencing and Analysis. Cold Spring Harb Protoc 11:951-969
Lee SM, Rhee SH, Park KY (1997) Antimutagenic effect of various cruciferous vegetables in salmonella assaying system. J Food Hyg Saf 12:321-327
Li Y, Zhang T, Korkaya H, Liu S, Lee HF, Newman B, Yu Y, Clouthier SG, Schwartz SJ, Wicha MS, Sun D (2010) Sulforaphane, a dietary component of broccoli/broccoli sprouts, inhibits breast cancer stem cells. Clin Cancer Res 16:2580-2590

상세보기
Li Z, Liu Y, Li L, Fang Z, Yang L, Zhuang M, Zhang Y, Lv H (2019) Transcriptome reveals the gene expression patterns of sulforaphane metabolism in broccoli florets. PLoS ONE 14(3): e0213902

상세보기
Lim HS (2002) The study for contents of sinigrin in dolsan leaf mustard kimchi during fementation periods. Korean J Life Sci 12:523-527

원문보기 상세보기
Liu S, Liu Y, Yang X, Tong C, Edwards D, Parkin IAP, Zhao M, Ma J, Yu J, Huang S, Wang X, Wang J, Lu K, Fang Z, Bancroft I, Yang TJ, Hu Q, Wang X, Yue Z, Li H, Yang L, Wu J, Zhou Q, Wang W, King GJ, Pires JC, Lu C, Wu Z, Sampath P, Wang Z, Guo H, Pan S, Yang L, Min J, Zhang D, Jin D, Li W, Belcram H, Tu J, Guan M, Qi C, Du D, Li J, Jiang L, Batley J, Sharpe AG, Park BS, Cheng PRF, Waminal NE, Huang Y, Dong C, Wang L, Li J, Hu Z, Zhuang M, Huang Y, Huang J, Shi J, Mei D, Liu J, Lee TH, Wang J, Jin H, Li Z, Li X, Zhang J, Xiao L, Zhou Y, Liu Z, Liu X, Qin R, Tang X, Liu W, Wang Y, Zhang Y, Lee J, Kim HH, Denoeud F, Xu X, Liang X, Hua W, Wang X, Wang J, Chalhoub B, Paterson AH (2014) The Brassica oleracea genome reveals the asymmetrical evolution of polyploid genomes. Nat Commun 5:3930

상세보기
Moon J, Jeong MJ, Lee SI, Lee JG, Hwang H, Yu J, Kim YR, Park SW, Kim JA (2015) Effect of LED mixed light conditions on the glucosinolate pathway in Brassica rapa. J Plant Biotechnol 42:245-256

원문보기 상세보기
Parkin IAP, Koh C, Tang H, Robinson SJ, Kagale S, Clarke WE, Town CD, Nixon J, Krishnakumar V, Bidwell SL, Denoeud F, Belcram H, Links MG, Just J, Clarke C, Bender T, Huebert T, Mason AS, Pires JC, Barker G, Moore J, Walley PG, Manoli S, Batley J, Edwards D, Nelson MN, Wang X, Paterson AH, King G, Bancroft I, Chalhoub B, Sharpe AG (2014) Transcriptome and methylome profiling reveals relics of genome dominance in the mesopolyploid Brassica oleracea. Genome Biol 15:R77

상세보기
Poiroux-Gonord F, Bidel LP, Fanciullino AL, Gautier H, Lauri-Lopez F, Urban L (2010) Health benefits of vitamins and secondary metabolites of fruits and vegetables and prospects to increase their concentrations by agronomic approaches. J Agr Food Chem 58:12065-12082

상세보기
Rajashekar CB, Carey EE, Zhao X, Oh MM (2009) Health-promoting phytochemicals in fruits and vegetables: Impact of abiotic stresses and crop production practices. Functional Plant Sci Biotechnol 3:30-38
Rangkadilok N, Nicolas ME, Bennett RN, Eagling DR, Premier RR, Taylor PW (2004) The effect of sulfur fertilizer on glucoraphanin levels in broccoli (B. oleracea L. var. italica) at different growth stages. J Agric Food Chem 52: 2632-2639

상세보기
Robin AHK, Yi GE, Laila R, Yang K, Park JI, Kim HR, Nou IS (2016) Expression profiling of glucosinolate biosynthetic genes in Brassica oleracea L. var. capitata inbred lines reveals their association with glucosinolate content. Molecules 21:787-805

상세보기
Schlichting I, Berendzen J, Chu K, Stock AM, Maves SA, Benson DE, Sweet RM, Ringe D, Petsko GA, Sligar SG (2000) The catalytic pathway of cytochrome P450cam at atomic resolution, Science 287:1615-1622

상세보기
Schreiner M (2005) Vegetable crop management strategies to increase the quantity of phytochemicals. European J Nutr 44:85-94

상세보기
Soto A, Ruiz KB, Ziosi V, Costa G, Torrigiani P (2012) Ethylene and auxin biosynthesis and signaling are impaired by methyl jasmonate leading to a transient slowing down of ripening in peach fruit. J Plant Physiol 169:1858-1865

상세보기
Sun B, Liu N, Zhao Y, Yan H, Wang Q (2011) Variation of glucosinolates in three edible parts of Chinese kale (Brassica alboglabra Bailey) varieties. Food Chem 124:941-947

상세보기
Wang H, Wu J, Sun S, Liu B, Cheng F, Sun R, Wang X (2011) Glucosinolate biosynthetic genes in Brassica rapa. Gene 487:135-142

상세보기
Xing M, Lv H, Ma J, Xu D, Li H, Yang L, Kang J, Wang X, Fang Z (2016) Transcriptome profiling of resistance to Fusarium oxysporum f. sp. conglutinans in Cabbage (Brassica oleracea) roots. PLoS One 11(2):e0148048

상세보기
Yukimune Y, Tabata H, Higashi Y, Hara Y (1996) Methyl jasmonate-induced overproduction of paclitaxel and ba-ccatin III in Taxus cell suspension cultures. Nat Biotechnol 14:1129-1132

상세보기
Zhang Y, Talalay P (1994) Anticarcinogenic activities of organic isothiocyanates: Chemistry and mechanisms. Cancer Res 54:1976-1981

상세보기
Zhang Z, Ober JA, Kliebenstein DJ (2006) The gene controlling the quantitative trait locus epithiospecifier modifier1 alters glucosinolate hydrolysis and insect resistance in Arabidopsis. Plant Cell 18:1524-1536

상세보기
Zhao Z, Zhang W, Stanley BA, Assmann SM (2008) Functional proteomics of Arabidopsis thaliana guard cells uncovers new stomatal signaling pathways. Plant Cell 20:3210-3226

상세보기

저자의 다른 논문 :

LOADING...

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증