선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- For the analogs, the change in hydrophobicity is considered to be more crucial than the increase of net positive charge. Furthermore, to evaluate the hemolytic effect of the periplanetasin-4 analogs on human erythrocytes, their hemolytic activities were evaluated by measuring the amount of hemoglobin released from treated erythrocytes. The hemolytic effect of periplanetasin-4 on human erythrocytes was lower according to previous study [8].
- Gel filtration chromatography on a Sephadex G-50 column was performed to separate LUVs from un-capsulated dye. Leakage of capsulated calcein or FDs from the liposome was monitored by measuring the intensity of fluorescence at an excitation wavelength of 494 nm and an emission wavelength of 520 nm using a spectrofluorophotometer (Shimadzu, RF-5301PC, Shimadzu, Japan). Triton X-100 (0.
- To evaluate the pore sizes of periplanetasin-4 and its analogs, the release of fluorescent dyes from the LUVs was monitored by measuring the fluorescence intensity at an excitation wavelength of 490 nm and an emission wavelength of 520 nm with a spectrofluorophotometer (Shimadzu, RF-5301PC, Shimadzu). Subsequently, soluble fluorescent molecules, including calcein, FD4 and FD10, were included to a final concentration of 2 μM, 0.
대상 데이터
- Analytical and preparative reverse-phase HPLC runs were performed with a Shimadzu 20A or 6A gradient system. Data were collected using an SPD-20A detector at 230 nm. Chromatographic separations were achieved with a 1%/min linear gradient of buffer B in A (A = 0.
- Enterococcus faecium (ATCC 19434), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), and Salmonella enteritidis (ATCC 13076) were obtained from the American Type Culture Collection (ATCC; USA). Staphylococcus epidermidis (KCTC 1917), Streptococcus mutans (KCTC 3065) and Salmonella typhimurium (KCTC 1926) were obtained from the Korean Collection for Type Cultures (KCTC). Escherichia coli (BW25113) was obtained from the Coli Genetic Stock Center.
데이터처리
- Statistical significance was determined using Student’s t-test.
이론/모형
- All peptides were chemically synthesized by Anygen (Korea), using the solid-phase peptide synthesis method with Fmoc (9- fluorenyl-methoxycarbonyl) chemistry. Periplanetasin-4 and its analogs were manually synthesized.
- The peptides were purified using preparative reverse-phase high-performance liquid chromatography (RP-HPLC) on C18 columns (20 × 250 mm; Shim-pack; Shimadzu, Japan). The purity of the peptides was verified with analytical RP-HPLC, and the peptide masses were confirmed using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDITOF MS; Shimadzu). The course of the reaction was monitored using HPLC.
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