[논문]조록나무 잎 에틸 아세테이트 분획물의 아데노바이러스 36에 대한 항바이러스 효과

김혜란; 양은주; 장정현; 장경수

doi:10.5352/jls.2020.30.3.221

조록나무 잎 에틸 아세테이트 분획물의 아데노바이러스 36에 대한 항바이러스 효과
Antiviral Effects of Ethyl Acetate Fraction of Distylium racemosum Leaf Extract on Adenovirus 36 원문보기

생명과학회지 = Journal of life science, v.30 no.3, 2020년, pp.221 - 229

김혜란 (대구한의대학교 임상병리학과) , 양은주 (대구한의대학교 임상병리학과) , 장정현 (대구한의대학교 임상병리학과) , 장경수 (부산가톨릭대학교 임상병리학과)

초록
AI-Helper

비만의 원인은 유전적, 대사적 및 환경적 요인 등 다양한 가운데 특정 세균 및 바이러스 감염에 의해 비만이 유도되는 것이 확인되며 이러한 비만은 감염성 비만이라고 한다. 또한 감염성 비만을 유발하는 병원체 가운데 아데노바이러스 36이 밝혀졌다. 현재 비만 예방 및 치료제 개발에 대한 연구가 활발히 진행되는 가운데 감염성 비만의 예방 및 치료제 개발에 대한 연구는 불충불한 실정이다. 이와 관련하여 본 연구에서는 제주에 자생하는 조록나무 잎을 이용하여 분획물을 제조하고, 비만을 유도하는 사람아데노바이러스 36 증식에 미치는 영향을 평가하고자 한다. A549세포에 사람아데노바이러스 36을 감염시키고, 조록나무 잎 에틸 아세테이트 분획물을 처리하여 바이러스 역가를 측정하였다. 그 결과, 조록나무 잎 에틸 아세테이트 분획물을 처리한군에서 농도 의존적으로 바이러스 증식 억제능을 확인하였다. 또한 조록나무 에틸 아세테이트 분획물의 물질 분리를 한 결과, quercitrin이 확인되었으며, 표준물질 quercitrin의 사람아데노바이러스 36에 대한 증식 억제능을 확인하였다. 이러한 결과 조록나무 잎 에틸 아세테이트 분획물은 감염성 비만 치료제 개발을 위한 효과적인 후보 물질로 사료되며, 본 연구는 감염성 비만 치료제 개발을 위한 기초 자료로 유용할 것으로 보인다.

Abstract ▼ AI-Helper

Distylium racemosum is an evergreen tree growing wild on Jeju Island, which has been reported to exert biological activity. Obesity is induced by genetic, metabolic, environmental, and other factors. Among these, certain bacterial and viral infections have been shown to cause obesity, which is known as infectobesity. Human adenovirus (HAdV)-36 is one of the viruses that are known to cause infectobesity in humans. Unlike extensive research on preventing obesity and developing anti-obesity drugs, little research has been conducted specifically on the prevention and treatment of infectobesity. Therefore, this study aimed to evaluate the effects of phytochemicals from D. racemosum on the replication of HAdV-36. A549 cells infected with HAdV-36 were treated with an ethyl acetate fraction of a D. racemosum leaf extract (DRE), and the virus titer was calculated based on the hemagglutination (HA) titer. The results showed a concentration-dependent inhibitory effect of DRE treatment on HA titers. DRE treatment was also found to inhibit the cytopathic effects of the virus and the expression of viral genes. Quercitrin was identified as the constituent of DRE exerting an inhibitory effect on HAdV-36 replication. This study shows that DRE can be used as a candidate substance for the development of treatment for HAdV-36 infections. In addition, this study provides a basis to further investigate DRE for the development of anti-infectobesity medication.

주제어

표/그림 (10)

표 Table 1. Primers used in this study
표 Table 2. Conditions for analysis of DRE
$Fig. 1. Effects of ethyl acetate fraction of Distylium racemosum (DRE) on A549 cell viability.$ 그림 Fig. 1. Effects of ethyl acetate fraction of Distylium racemosum (DRE) on A549 cell viability.
$Fig. 2. Intracellular antiviral activity of ethyl acetate fraction from Distylium racemosum (DRE).$ 그림 Fig. 2. Intracellular antiviral activity of ethyl acetate fraction from Distylium racemosum (DRE).
$Fig. 3. Extracellular antiviral activity of ethyl acetate fraction from Distylium racemosum (DRE).$ 그림 Fig. 3. Extracellular antiviral activity of ethyl acetate fraction from Distylium racemosum (DRE).
$Fig. 4. Effects of ethyl acetate fraction from Distylium racemosum (DRE) on hemagglutination inhibition.$ 그림 Fig. 4. Effects of ethyl acetate fraction from Distylium racemosum (DRE) on hemagglutination inhibition.
$Fig. 5. Effects of ethyl acetate fraction from Distylium racemosum (DRE) on cytopathogenic reduction.$ 그림 Fig. 5. Effects of ethyl acetate fraction from Distylium racemosum (DRE) on cytopathogenic reduction.
그림 Fig. 6. High performance liquid chromatography (HPLC) analysis.
그림 Fig. 7. Effects of quercitrin on A549 cell viability and Hemagglutination titer.
그림 Fig. 8. Antiviral activity of quercitrin.

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

racemosum against ribonuclease H, there have been few studies regarding its anti-infectobesity effects [12]. This study aimed to evaluate the effects of an ethyl acetate fraction from a D. racemosum leaf extract (DRE) on HAdV-36 replication and to use the data as a basis for the development of natural treatment for infectobesity.
This study revealed suppressive effects of DRE on replication of HAdV-36, an obesity-inducing virus. The results can serve as a basis for the development of infectobesity treatment.

제안 방법

To confirm the antiviral effect of DRE, HA titer and CPE were performed. The virus titer was determined using the HA titer.
To examine the antiviral activity of DRE against HAdV36, we evaluated cytotoxic effects of DRE. Effects of DREon the viability of A549 cells were determined by the MTT assay.

대상 데이터

A549 (ATCC® CCL-185™) cells were obtained from the American Type Culture Collection (Manassas, VA, USA).
D. racemosum leaves, native to the Jeju island, were washed with distilled water three times and dried in shade for 2 weeks. Then, the leaves were powdered and used as an extraction specimen.
Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and penicillin–streptomycin (PS) were obtained from Welgene (Daegu, Korea).

이론/모형

5, 25, 50, and 100 μg/ml) of DRE for 48 hr. Cell viability was measured by MTT assay. Data are expressed as mean ± standard deviation (n=3).
The cell viability was evaluated using MTT assay [9]. A549 cells were cultured in DMEM containing 10% FBS and 1% PS at 37℃ in a 5% CO₂ incubator, then diluted to 1×10⁵cells/ml, and 100 μl of the cell suspension was plated per well in a 96-well plate.
The level of relative gene expression was determined by the 2-ΔΔCT method.

성능/효과

In this study, the anti-viral effect against HAdV-36 was identified with quercitrin, but it showed lower anti-virus effect than DRE treatment. These results are thought to be indicative of the anti-viral effect by mixing quercitrin and other unknown substances contained in DRE.

후속연구

Therefore, additional analysis of substances in DRE is required, and after analyzing the substances, substances that exhibit antiviral effects need to be identified. In addition, the further study should be performed for pathway on viral replication.
have shown an antiviral effect of a mulberry extract on HAdV-36, but there have been no other studies on antiviral effects against HAdV-36 [18]. Thus, more research is needed to search for natural products that are highly effective anti-infectobesity drugs.

참고문헌 (29)

Atkinson, R. L., Dhurandhar, N. V., Allison, D. B., Bowen, R. L., Israel, B. A. and Albu, J. B. 2005. Human adenovirus-36 is associated with increased body weight and paradoxical reduction of serum lipids. Int. J. Obes. (Lond). 29, 281-286.

상세보기
Comalada, M., Camuesco, D., Sierra, S., Ballester, I., Xaus, J., Galvez, J. and Zarzuelo, A. 2005. In vivo quercitrin anti-inflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF- ${\kappa}B$ pathway. Eur. J. Immunol. 35, 584-592.

상세보기
Dhurandhar, N. V., Israel, B. A., Kolesar, J. M., Mayhew, G. F., Cook, M. E. and Atkinson, R. L. 2000. Increased adiposity in animals due to a human virus. Int. J. Obes. Relat. Metab. Disord. 24, 989-996.

상세보기
Galvez, J., Crespo, M. E., Jimenez, J., Suarez, A. and Zarzuelo, A. 1993. Antidiarrhoeic activity of quercitrin in mice and rats. J. Pharm. Pharmacol. 45, 157-159.

상세보기
Hasan, S., Ahmed, I., Mondal, S., Masud, M. M., Sadhu, S. K. and Ishibashi, M. 2006. Antioxidant, antinociceptive activity and general toxicity study of Dendrophthoe falcata and isolation of quercitrin as the major component. Orient. Pharm. Exp. Med. 6, 355-360.

원문보기 상세보기
Hill, J. O. and Peters, J. C. 1998. Environmental contributions to the obesity epidemic. Science 280, 1371-1374.

상세보기
Huang, Y. W., Liu, Y., Dushenkov, S. and Ho, C. T. 2009. Anti-obesity effects of epigallocatechin-3-gallate, orange peel extract, black tea extract, caffeine and their combinations in a mouse model. J. Funct. Foods 2009, 304-310.
Jeon, H. J., Choi, H. S., Lee, O. H., Jeon, Y. J. and Lee, B. Y. 2012. Inhibition of reactive oxygen species (ROS) and nitric oxide (NO) by Gelidium elegans using alternative drying and extraction conditions in 3T3-L1 and RAW 264.7 cells. Prev. Nutr. Food. Sci. 17, 122-128.

원문보기 상세보기
Jo, H. J., Shin, Y. S., Park, G. N. and Chang, K. S. 2013. Inhibition of hepatitis C virus (HCV) genotype 2a RNA replication by extracts of medicinal herbs with anti-cholesterol effects. J. Med. Plant. Res. 7, 1089-1099.
Kim, S. K., Kim, H. J., Hur, K. Y., Choi, S. H., Ahn, C. W., Lim, S. K., Kim, K. R., Lee, H. C., Huh, K. B. and Cha, B. S. 2004. Visceral fat thickness measured by ultrasonography can estimate not only visceral obesity but also risks of cardiovascular and metabolic diseases. Am. J. Clin. Nutr. 79, 593-599.

상세보기
Kim, S. S., Kim, J. Y., Lee, N. H. and Hyun, C. G. 2008. Antibacterial and anti-inflammatory effects of Jeju medicinal plants against acne-inducing bacteria. J. Gen. Appl. Microbiol. 54, 101-106.

상세보기
Lee, W. Y., Ahn, J. K., Park, Y. K., Park, S. Y., Kim, Y. M. and Rhee, H. I. 2004. Inhibitory effects of proanthocyanidin extracted from Distylium racemosum on a-amylase and a-glucosidase activities. Kor. J. Pharmacogn. 35, 271-275.
Lupini, C., Cecchinato, M., Scagliarini, A., Graziani, R. and Catelli, E. 2009. In vitro antiviral activity of chestnut and quebracho woods extracts against avian reovirus and metapneumovirus. Res. Vet. Sci. 87, 482-487.

상세보기
Middleton, E. Jr., Kandaswami, C. and Theoharides, T. C. 2000. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol. Rev. 52, 673-751.

상세보기
Moradi, M. T., Karimi, A., Rafieian Kopaei, M. and Fotouhi, F. 2017. In vitro antiviral effects of Peganum harmala seed extract and its total alkaloids against influenza virus. Microb. Pathog. 110, 42-49.

상세보기
Morfin, F., Dupuis-Girod, S., Mundweiler, S., Falcon, D., Carrington, D., Sedlacek, P., Bierings, M., Cetkovsky, P., Kroes, A. C., van Tol, M. J. and Thouvenot, D. 2005. In vitro susceptibility of adenovirus to antiviral drugs is species-dependent. Antivir. Ther. 10, 225-229.

상세보기
Na, H. N., Hong, Y. M., Kim, J., Kim, H. K., Jo, I. and Nam, J. H. 2010. Association between human adenovirus-36 and lipid disorders in Korean schoolchildren. Int. J. Obes. (Lond). 34, 89-93.

상세보기
Na, H. N., Park, S., Jeon, H. J., Kim, H. B. and Nam, J. H. 2014. Reduction of adenovirus 36-induced obesity and inflammation by mulberry extract. Microbiol. Immunol. 58, 303-306.

상세보기
Nwodo, U. U., Ngene, A. A., Iroegbu, C. U., Onyedikachi, O. A., Chigor, V. N. and Okoh, A. I. 2011. In vivo evaluation of the antiviral activity of Cajanus cajan on measles virus. Arch. Virol. 156, 1551-1557.

상세보기
Park, Y. K., Lee, W. Y., Ahn, J. K., Lee, H. J., Chin, H. S. and Kwon, Y. J. 2003. Antioxidant compounds from Distylium racemosum leaves. J. Kor. Wood Sci. Technol. 31, 67-72.
Pasarica, M. and Dhurandhar, N. V. 2007. Infectobesity: obesity of infectious origin. Adv. Food. Nutr. Res. 52, 61-102.

상세보기
Serkedjieva, J. and Hay, A. J. 1998. In vitro anti-influenza virus activity of a plant preparation from Geranium sanguineum L. Antivir. Res. 37, 221-230.
van Ginneken, V., Sitnyakowsky, L. and Jeffery, J. E. 2009. Infectobesity: viral infections (especially with human adenovirus-36: Ad-36) may be a cause of obesity. Med. Hypotheses 2, 383-388.
Vangipuram, S. D., Sheele, J., Atkinson, R. L., Holland, T. C. and Dhurandhar, N. V. 2004. A human adenovirus enhances preadipocyte differentiation. Obes. Res. 12, 770-777.

상세보기
Voss, J. D. and Dhurandhar, N. V. 2017. Viral infections and obesity. Curr. Obes. Rep. 6, 28-37.

상세보기
Whigham, L. D., Israel, B. A. and Atkinson, R. L. 2006. Adipogenic potential of multiple human adenoviruses in vivo and in vitro in animals. Am. J. Physiol. Regul. Integr. Comp. Physiol. 290, R190-194.

상세보기
Wigand, R., Gelderblom, H. and Wadell, G. 1980. New human adenovirus (candidate adenovirus 36), a novel member of subgroup D. Arch. Virol. 64, 225-233.

상세보기
Xu, H. X., Wam, M., Dong, H., But, P. P. and Foo, L. Y. 2000. Inhibitory activity of flavonoids and tannins against HIV-1 protease. Biol. Pharmaceut. Bull. 23, 1072-1076.

상세보기
Yang, E. J., Ham, Y. M., Lee, W. J., Lee, N. H. and Hyun, C. G. 2013. Anti-inflammatory effects of apo-9'-fucoxanthinone from the brown alga, Sargassum muticum. Daru 21, 62.

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증