Petsong, Kantiya
(Department of Food Technology, Prince of Songkla University)
,
Vongkamjan, Kitiya
(Department of Food Technology, Prince of Songkla University)
,
Ahn, Juhee
(Department of Medical Biomaterials Engineering, Kangwon National University)
본 연구는 항생제 내성 Salmonella Typhimurium CCARM 8009을 저해하기 위한 phage와 항생제 조합처리의 효과를 평가하였다. 디스크 확산법과 액체배지 희석법에 의해 phage와 항생제의 상승 저해효과를 측정하였고 배양을 통한 항생제 내성 유도를 평가하였다. Phage를 처리한 cefotaxime, chloramphenicol, ciprofloxacin, erythromycin의 디스크의 저해 구역은 각각 13.6%, 19.3%, 12.7%, 78.8%로 증가되었다. Phage와 항생제 조합 처리에 의해 tetracycline, chloramphenicol, ciprofloxacin, erythromycin, streptomycin의 최소생육억제농도는 각각 64, 4, 0.0078, 64, 256 mg/mL으로 감소되었다. Phage와 항생제의 조합 처리는 항생제 내성 S. Typhimurium CCARM 8009을 효과적으로 저해하였다 (4 log reduction). 본 결과는 phage와 항생제의 조합처리는 항생제 내성균을 제어하기 위한 방법으로 충분히 응용가치가 높음을 보여주고 있다.
본 연구는 항생제 내성 Salmonella Typhimurium CCARM 8009을 저해하기 위한 phage와 항생제 조합처리의 효과를 평가하였다. 디스크 확산법과 액체배지 희석법에 의해 phage와 항생제의 상승 저해효과를 측정하였고 배양을 통한 항생제 내성 유도를 평가하였다. Phage를 처리한 cefotaxime, chloramphenicol, ciprofloxacin, erythromycin의 디스크의 저해 구역은 각각 13.6%, 19.3%, 12.7%, 78.8%로 증가되었다. Phage와 항생제 조합 처리에 의해 tetracycline, chloramphenicol, ciprofloxacin, erythromycin, streptomycin의 최소생육억제농도는 각각 64, 4, 0.0078, 64, 256 mg/mL으로 감소되었다. Phage와 항생제의 조합 처리는 항생제 내성 S. Typhimurium CCARM 8009을 효과적으로 저해하였다 (4 log reduction). 본 결과는 phage와 항생제의 조합처리는 항생제 내성균을 제어하기 위한 방법으로 충분히 응용가치가 높음을 보여주고 있다.
In this study, we investigated the efficacy of Salmonella phage P22 combined with antibiotics to inhibit antibiotic-resistant S. Typhimurium CCARM 8009. The synergistic effect of phage P22 and antibiotics was evaluated by using disk diffusion and broth dilution assays. The development of Antimicrobi...
In this study, we investigated the efficacy of Salmonella phage P22 combined with antibiotics to inhibit antibiotic-resistant S. Typhimurium CCARM 8009. The synergistic effect of phage P22 and antibiotics was evaluated by using disk diffusion and broth dilution assays. The development of Antimicrobial resistance was determined after time-kill assay. The antibiotic susceptibility assay showed the inhibition zone sizes around the antibiotic disks were increased up to 78.8% in the presence of phage (cefotaxime; 13.6%, chloramphenicol; 19.3%, ciprofloxacin; 12.7% and erythromycin; 78.8%). The minimum inhibitory concentration values of the combination treatment significantly decreased from 256 to 64 mg/mL for tetracycline, 8 to 4 mg/mL for chloramphenicol, 0.0156 to 0.0078 mg/mL for ciprofloxacin, 128 to 64 mg/mL for erythromycin and 512 to 256 mg/mL for streptomycin. The number of S. Typhimurium CCARM 8009 was approximately 4-log lower than that of the control throughout the combination treatment with phage P22 and ciprofloxacin delete at 37℃ for 20 h. The results indicate that the development of antimicrobial resistance in S. Typhimurium could be reduced in the presence of phage treatment. This study provides promising evidence for the phage-antibiotic combination as an effective treatment to control antibiotic-resistant bacteria.
In this study, we investigated the efficacy of Salmonella phage P22 combined with antibiotics to inhibit antibiotic-resistant S. Typhimurium CCARM 8009. The synergistic effect of phage P22 and antibiotics was evaluated by using disk diffusion and broth dilution assays. The development of Antimicrobial resistance was determined after time-kill assay. The antibiotic susceptibility assay showed the inhibition zone sizes around the antibiotic disks were increased up to 78.8% in the presence of phage (cefotaxime; 13.6%, chloramphenicol; 19.3%, ciprofloxacin; 12.7% and erythromycin; 78.8%). The minimum inhibitory concentration values of the combination treatment significantly decreased from 256 to 64 mg/mL for tetracycline, 8 to 4 mg/mL for chloramphenicol, 0.0156 to 0.0078 mg/mL for ciprofloxacin, 128 to 64 mg/mL for erythromycin and 512 to 256 mg/mL for streptomycin. The number of S. Typhimurium CCARM 8009 was approximately 4-log lower than that of the control throughout the combination treatment with phage P22 and ciprofloxacin delete at 37℃ for 20 h. The results indicate that the development of antimicrobial resistance in S. Typhimurium could be reduced in the presence of phage treatment. This study provides promising evidence for the phage-antibiotic combination as an effective treatment to control antibiotic-resistant bacteria.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
문제 정의
The effective inactivation of Salmonella growth was significantly higher in the treatment of phage-antibiotic combination than those treatments of phage alone or antibiotic alone. This study provides evidence that phages could link to the key functional properties which could decrease the expression of antimicrobial resistance after treatments. Since the function of antibiotics still provides the benefit on microbial control and the advantages of phage application are of interest, this study suggests that PAS should be applied as the effective alternative tool for reducing and controlling antibiotic-resistant bacteria.
가설 설정
Whereas phage particles synthesize the new progenies by using bacterial DNA machinery20). The phenomenon observed in this study could be hypothesized that the mechanism of phage to lyse bacterial cells might be affected to genes related antimicrobial resistance in bacteria, resulting the less expression of antimicrobial resistance development. The observed results indicating that the presence of phage particles in the system could decrease the virulence of antimicrobial resistance development in bacterial cells.
대상 데이터
All experiments were conducted in duplicate for three replicates. The Analysis of variance (ANOVA) was used todetermine significant mean differences at P<0.
Salmonella bacteriophage P22 (ATCC 97541) was propagated in TSB containing the suggested host S. Typhimurium LT2 (ATCC 19585), purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) at the multiplicity of infection (MOI) of 0.1. The mixture was incubated at 37℃ for 24 h.
In this study, Salmonella enterica subsp. enterica serovar Typhimurium CCRAM8009 from the Culture Collection of Antibiotic Resistant Microbes (CCARM, Seoul, Korea) was used as the targeted bacteria. For each experiment, Salmonella strain was cultured in trypticase soy broth (TSB; BD, Becton, Dickinson and Co.
데이터처리
Comparison of means was carried out by Duncan’s multiple range tests.
The Analysis of variance (ANOVA) was used todetermine significant mean differences at P<0.05 using the Statistical Package for Social Science (SPSS 10.0 for windows, SPSS Inc., Chicago, IL, USA).
이론/모형
In this study, agar disk diffusion assay was used to determine the changes of antimicrobial resistance in S. Typhimurium CCARM 8009 after the time-kill assay. S.
성능/효과
In conclusion, this study demonstrates the effective antimicrobial efficacy of phage and antibiotic combination to reduce the number of antibiotic-resistant Salmonella and the occurrence of antimicrobial resistance development after treatment. The results showed that presence of phage particles in the system of antibiotic treatment could provide the positive effects to solve the concerned issue.
In this study, the treatment of phage alone showed the remarkable potential to decrease antimicrobial resistance development in bacterial cells. Whereas, the treatment of antibiotic alone showed the highest percentage of antimicrobial resistance development among all treatments.
4 to 3 log cfu/mL). Overall, the remarkable decrease was obtained from the combination treatment as indicated by the decrease of the number of S. Typhimurium CCARM8009 by approximately 4 log cfu/mL, when compared to the control, throughout the 20-h incubation period.
The study reported that Salmonella isolates conferring resistance to ≥5 antibiotics (i.e. ceftriaxone, ciprofloxacin, streptomycin, tetracycline and erythromycin) accounted for more than 66,000 illnesses from 2009 to 2011 in the U.S5).
참고문헌 (20)
CDC, (2020. February 1) Salmonella. 2019. Available online: https://www.cdc.gov/salmonella/general/index.html#two
Eng, S.K., Pusparajah, P., Ab Mutalib, N.S., Ser, H.L., Chan, K.G., Lee, L.H., Salmonella: A review on pathogenesis, epidemiology and antibiotic resistance. Front. Life Sci., 8, 284-293 (2015).
Martin, M.J., Thottathil, S.E., Newman, T.B., Antibiotics overuse in animal agriculture: A call to action for health care providers. Am. J. Public Health, 105, 2409-2410 (2015).
V T Nair, D., Venkitanarayanan, K., Kollanoor Johny, A., Antibiotic-resistant Salmonella in the food supply and the potential role of antibiotic alternatives for control. Foods, 7, 167 (2018).
Lin, D.M., Koskella, B., Lin, H.C., Phage therapy: An alternative to antibiotics in the age of multi-drug resistance. World J. Gastrointest. Pharmacol. Therapeut., 8, 162-173 (2017).
Petsong, K., Uddin, M.J., Vongkamjan, K., Ahn, J., Combined effect of bacteriophage and antibiotic on the inhibition of the development of antibiotic resistance in Salmonella typhimurium. Food Sci. Biotechnol., 27, 1239-1244 (2018).
Bielke, L., Higgins, S., Donoghue, A., Donoghue, D., Hargis, B.M., Salmonella host range of bacteriophages that infect multiple genera. Poult. Sci., 86, 2536-2540 (2007).
Lin, H.L., Lin, C.C., Lin, Y.J., Lin, H.C., Shih, C.M., Chen, C.R., Huang, R.N., Kuo, T.C., Revisiting with a relative-density calibration approach the determination of growth rates of microorganisms by use of optical density data from liquid cultures. Appl. Environ. Microbiol., 76, 1683-1685 (2010).
Akturk, E., Oliveira, H., Santos, S.B., Costa, S., Kuyumcu, S., Melo, L.D.R., Azeredo, J., Synergistic action of phage and antibiotics: Parameters to enhance the killing efficacy against mono and dual-species biofilms. Antibiotics, 8, 103 (2019).
Valerio, N., Oliveira, C., Jesus, V., Branco, T., Pereira, C., Moreirinha, C., Almeida, A., Effects of single and combined use of bacteriophages and antibiotics to inactivate Escherichia coli. Virus Res., 240, 8-17 (2017).
※ AI-Helper는 부적절한 답변을 할 수 있습니다.