[논문]Padina boryana, a brown alga from the Maldives: inhibition of α-MSH-stimulated melanogenesis via the activation of ERK in B16F10 cells

Jayawardena, Thilina U.; Sanjeewa, K.K. Asanka; Kim, Hyun-Soo; Lee, Hyo Geun; Wang, Lei; Lee, Dae-Sung; Jeon, You-Jin

doi:10.1186/s41240-020-00154-x

Padina boryana, a brown alga from the Maldives: inhibition of α-MSH-stimulated melanogenesis via the activation of ERK in B16F10 cells 원문보기

Fisheries and aquatic sciences, v.23 no.3, 2020년, pp.8.1 - 8.9

Jayawardena, Thilina U. (Department of Marine Life Sciences, Jeju National University) , Sanjeewa, K.K. Asanka (Department of Marine Life Sciences, Jeju National University) , Kim, Hyun-Soo (National Marine Biodiversity Institute of Korea) , Lee, Hyo Geun (Department of Marine Life Sciences, Jeju National University) , Wang, Lei (Department of Marine Life Sciences, Jeju National University) , Lee, Dae-Sung (National Marine Biodiversity Institute of Korea) , Jeon, You-Jin (Department of Marine Life Sciences, Jeju National University)

Abstract ▼ AI-Helper

Background: The present study investigates the potent skin whitening ability of ethanol extract from the brown alga, Padina boryana (PBE) which was collected in the shores of Fulhadhoo Island, the Maldives, and its specific pathways of action. The effect of PBE which contains a rich amount of polyphenols was evaluated using B16F10 murine melanoma cells and provides insight to the underlying mechanisms with reference to the inhibition of melanin formation. Methods: Melanin synthesis and cellular tyrosinase inhibition were assessed in the α-MSH-stimulated melanocytes. Melanogenic pathway-related protein expressions were investigated via Western blotting. ERK 42/44 was particularly examined considering its involvement in the melanogenic pathway. Further, RT-qPCR techniques were involved in gene expression analysis. Results: PBE dose-dependently inhibited the cellular melanin synthesis and tyrosinase levels. Western blotting revealed the potential of PBE to downregulate microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related protein-1 and protein-2 (TRP-1 and TRP-2). Moreover, results explained the phosphorylation of ERK was sustained via PBE and hence declined the ultimate melanin synthesis. Gene expression analysis reinforced the results obtained. Conclusions: The study provides substantial evidence to express the potential of PBE to inhibit B16F10 melanoma cell melanin synthesis. Concisely, results suggest the ability of PBE to be involved in medicinal and cosmeceutical applications.

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표/그림 (4)

그림 Fig. 1 a Cytotoxic effect of 70% ethanolic extract from P. boryana (PBE) on B16F10 cells. b Cytotoxicity evaluation of α-MSH and PBE on B16F10 cells. Experiments were triplicated and represented as means ± SD (n = 3). *p < 0.05; **p < 0.01
그림 Fig. 2 PBE ability for melanin inhibition on α-MSH-stimulated B16F10 cells. Cultured cells were treated with 0~100 μg/mL of PBE and stimulated with α-MSH. a Following the incubation, melanin content was analyzed. Melanin content is represented compared to the non-treated group as percentage values. b Effects of PBE on the tyrosine production in B16F10 cells. Similarly, cells were seeded and samples and α-MSH were treated. After incubation, tyrosinase activity was assessed using the L-DOPA oxidation assay. Each assay was triplicated and the data points are represented as means ± SD (n = 3). *p < 0.05; **p < 0.01 (number sign denotes significance compared to control while asterisk represents significance compared to the α-MSH-treated group)
그림 Fig. 3 Expression of proteins and genes related to melanogenesis in B16F10 cells against PBE treatment. a Cells were exposed to α-MSH in the presence of 50 and100 μg/mL PBE or 100 μM arbutin, for 72 h. MITF, tyrosinase, TRP-1, and TRP-2 proteins were studied by Western blot. Internal control was β-actin. b Relevant quantitative data. Melanogenic gene expressions. c Tyrosinase and d MITF gene expression. Cells were exposed to α-MSH in the presence and absence of PBE and ERK inhibitor, PD98059. The mRNA levels were examined by RT-PCR. GAPDH was used as an internal control. Results represent as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 (number sign denotes significance compared to control while asterisk represents significance compared to the α-MSH-treated group)
그림 Fig. 4 Effect of P. boryana ethanolic extract on melanoma cells against specific ERK inhibitor. a ERK pathway associated protein and c tyrosinase expression in α-MSH-stimulated B16F10 cells. Cells were incubated with α-MSH with PBE in the absence or presence of ERK-specific inhibitor, PD98059. Protein expressions were analyzed by Western blotting, b, d relevant quantitative data. Data points and bars represent the means ± SD (n = 3) (*p < 0.05; **p < 0.01) (number sign denotes significance compared to control while asterisk represents significance compared to the α-MSH-treated group)

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