Kang, Kyung Soo
(Bio Division, Ovozen, Inc.)
,
Shin, Sangsu
(Department of Animal Biotechnology, Kyungpook National University)
,
Lee, Sang In
(Department of Animal Biotechnology, Kyungpook National University)
Cyclophosphamide, a cytotoxic anticancer agent, induces immunosuppression and has several adverse effects. N-acetylcysteine alleviates oxidative stress, liver injury, and intestinal tissue damage. The present study examined whether N-acetylcysteine modulates the adverse effects of cyclophosphamide i...
Cyclophosphamide, a cytotoxic anticancer agent, induces immunosuppression and has several adverse effects. N-acetylcysteine alleviates oxidative stress, liver injury, and intestinal tissue damage. The present study examined whether N-acetylcysteine modulates the adverse effects of cyclophosphamide in pigs. Miniature pigs (n = 15) were used as an experimental model to evaluate the effects of N-acetylcysteine treatment on immune reactions, liver injury, and oxidative stress after cyclophosphamide challenge. Corn-soybean meal based dietary treatments were as follows: control diet with either saline injection, cyclophosphamide injection, or 0.5% N-acetylcysteine and cyclophosphamide injection. N-acetylcysteine increased the number of immune cells and decreased TNF-α production after cyclophosphamide injection and decreased TNF-α, IFN-γ, NF-κB, and IL-8 expression and increased IL-10 expression in peripheral blood mononuclear cells. Serum levels of alanine transaminase and aspartate aminotransferase decreased, superoxide dismutase activity increased, and malondialdehyde activity decreased following N-acetylcysteine treatment after cyclophosphamide injection. N-acetylcysteine decreases immunosuppression, liver injury, and oxidative stress in cyclophosphamide-challenged miniature pigs. The present study suggests that N-acetylcysteine has therapeutic application in livestock for modulating immune reactions, liver injury, and oxidative stress.
Cyclophosphamide, a cytotoxic anticancer agent, induces immunosuppression and has several adverse effects. N-acetylcysteine alleviates oxidative stress, liver injury, and intestinal tissue damage. The present study examined whether N-acetylcysteine modulates the adverse effects of cyclophosphamide in pigs. Miniature pigs (n = 15) were used as an experimental model to evaluate the effects of N-acetylcysteine treatment on immune reactions, liver injury, and oxidative stress after cyclophosphamide challenge. Corn-soybean meal based dietary treatments were as follows: control diet with either saline injection, cyclophosphamide injection, or 0.5% N-acetylcysteine and cyclophosphamide injection. N-acetylcysteine increased the number of immune cells and decreased TNF-α production after cyclophosphamide injection and decreased TNF-α, IFN-γ, NF-κB, and IL-8 expression and increased IL-10 expression in peripheral blood mononuclear cells. Serum levels of alanine transaminase and aspartate aminotransferase decreased, superoxide dismutase activity increased, and malondialdehyde activity decreased following N-acetylcysteine treatment after cyclophosphamide injection. N-acetylcysteine decreases immunosuppression, liver injury, and oxidative stress in cyclophosphamide-challenged miniature pigs. The present study suggests that N-acetylcysteine has therapeutic application in livestock for modulating immune reactions, liver injury, and oxidative stress.
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제안 방법
Miniature pigs (n = 15) [MK strain; (Duroc × Yorkshire) × (Pot Valley × Berkshire) × Yucatan] with an average initial body weight of 22.3 ± 0.32 kg were used to evaluate the effects of dietary supplementation with NAC on effects caused by CTX injection in a 4-week trial.
Quantitative real-time PCR was performed using a 7500 Fast Real-Time PCR System (Applied Biosystems, Waltham, MA, USA) with the following conditions: 94℃ for 3 min, followed by 40 cycles at 94℃ for 30 s, 59℃–61℃ for 30 s, and 72℃ for 30 s.
Statistical significance of differences between treatments for immune cells, TNF-α production, inflammatory cytokine-related gene expression, liver function parameters, and oxidative stress markers was analyzed using the GLM in SAS.
데이터처리
Duncan’s multiple range test was used as a post hoc test to analyze differences between means, and a p-value of < 0.05 was considered statistically significant.
a–cIndicate significant differences between treatments as determined by Duncan's multiple range tests.
a,bIndicate significant differences between treatments as determined by Duncan's multiple range tests.
이론/모형
Effects of dietary supplementation with N-acetylcysteine (NAC) on oxidative stress markers. Serum SOD (A), MDA (B), and GPx (C) level of cyclophosphamide-induced immunosuppressed miniature pigs were determined using ELISA (n = 5). Error bars indicate the standard error of the mean (SEM).
The quantitative reverse transcription polymerase chain reaction (qRT-PCR) data were calculated using the 2−ΔΔCt method (n = 5).
성능/효과
In conclusion, dietary supplementation with NAC modulates immune cell populations and TNF-α levels in serum after CTX-induced immunosuppression in miniature pigs.
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