[논문]Analysis of Dibenzocyclooctadiene Lignans in Omija Wine and Cheong by Liquid Chromatography-Tandem Mass Spectrometry

Seo, Hyung-Ju; Ji, Seung-Bae; Kim, Sin-Eun; Lee, Gyung-Min; Moon, Seong-Hun; Jang, Dae-Sik; Liu, Kwang-Hyeon

doi:10.5478/msl.2020.11.2.30

[국내논문] Analysis of Dibenzocyclooctadiene Lignans in Omija Wine and Cheong by Liquid Chromatography-Tandem Mass Spectrometry 원문보기

Mass spectrometry letters, v.11 no.2, 2020년, pp.30 - 35

Seo, Hyung-Ju (BK21 Plus KNU Multi-Omics based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University) , Ji, Seung-Bae (BK21 Plus KNU Multi-Omics based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University) , Kim, Sin-Eun (BK21 Plus KNU Multi-Omics based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University) , Lee, Gyung-Min (BK21 Plus KNU Multi-Omics based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University) , Moon, Seong-Hun (OmyNara 609) , Jang, Dae-Sik (Department of Life and Nanopharmaceutical Sciences, KyungHee University) , Liu, Kwang-Hyeon (BK21 Plus KNU Multi-Omics based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University)

Abstract ▼ AI-Helper

Schisandra chinensis is a traditional herbal medicine that is widely spread in Korea, Japan, and China. The fruits of S. chinensis Bailon, known as omija in Korea, have traditionally been used for the treatment of coughs, fatigues, and insomnia. Up to now, there have been several reports for the identification of major lignan compounds and their quantitation in S. chinensis extracts. To the best of our knowledge, however, there is no report on the analysis of lignans in omija wine and omija cheong (sugared omija or omija sugar syrup). In the present study, seven dibenzocyclooctadiene lignans (gomisin A, gomisin B, gomisin C, gomisin N, schisandrin, deoxyschisandrin, and wuweizisu C) in omija wine and omija cheong were analyzed and quantitated using liquid chromatography-tandem mass spectrometry. Among seven lignans, pharmacologically active gomisin A, schisandrin, and deoxyschisandrin, which are major components in fruits of S. chinensis, were the most abundant lignans in omija wine and cheong. The content of lignan in omija wine was in the order: schisandrin > gomisin A > deoxyschisandrin > gomisin N > gomisin B > gomisin C > wuweizisu C. The concentration of deoxyschisandrin and gomisin N in omija wine was approximately 2.0- and 6.0-fold higher than for omija cheong. Additionally, this study provided a systematic identification of lignans in omija wine and cheong and indicated that the omija wine and cheong might be of value for their dietary application.

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표/그림 (5)

그림 Figure 1. Chemical structures of the dibenzocyclooctadiene lignans: (a) gomisin A; (b) gomisin B; (c) gomisin C; (d) gomisin N; (e) deoxyschisandrin; (f) wuweizisu C; and (g) schisandrin.
표 Table 1. Optimized selected reaction monitoring parameters for seven lignans used in all the assay.
표 Table 2. Calibration data of seven lignans and contents of seven lignans in omija cheong and omija wine.
그림 Figure 2. Product ion scan mass spectra of seven lignans (a) gomisin A, (b) gomisin B, (c) gomisin C, (d) gomisin N, (e) deoxyschisandrin, (f) wuweizisu C, and (g) schisandrin obtained using electrospray ionization mode.
그림 Figure 3. Representative selected reaction monitoring chromatograms of seven lignan standards (left column), omija cheong extracts (middle column), and omija wine extracts (right column).

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제안 방법

Omija wine and cheong extracts were analyzed using a LC-MS/MS (LCMS 8040, Shimadzu, Kyoto, Japan) equipped with an electrospray ionization interface. The samples were separated on a Kinetex C18 column (100 × 2.
In this study, we analyzed seven dibenzocyclooctadiene lignans (gomisin A, gomisin B, gomisin C, gomisin N, schisandrin, deoxyschisandrin, and wuweizisu C) which were reported as bioactive components in omija,¹⁷ in omija wine and cheong sample using LC-MS/MS. Precursorions for gomisin A, gomisin B, gomisin C, gomisin N, schisandrin, deoxyschisandrin, and wuweizisu C, and their corresponding product ions were determined from spectra obtained during the infusion of standard solutions into a mass spectrometer using an electrospray ionization source, which operated in positive ionization mode.
The present study is the first comparative and quantitative analyses of lignans in omija wine and cheong. The study identified seven dibenzocyclooctadiene lignans (gomisin A, gomisin B, gomisin C, gomisin N, schisandrin, deoxyschisandrin, and wuweizisu C) in omija wine and cheong. Pharmacologically active gomisin A, schisandrin, and deoxyschisandrin, which are major compounds in fruits of Schisandra chinensis, were the most abundant lignans in both samples.

대상 데이터

² In this paper, we have adopted the nomenclature of lignans from the recent review article.²² Gomisin B and gomisin C were purchased from Toronto Research Chemical (Toronto, Canada) and Sigma-Aldrich (St. Louis, MO, USA), respectively. All the other solvents were LC-MS grade (Fisher Scientific Co.

성능/효과

The study identified seven dibenzocyclooctadiene lignans (gomisin A, gomisin B, gomisin C, gomisin N, schisandrin, deoxyschisandrin, and wuweizisu C) in omija wine and cheong. Pharmacologically active gomisin A, schisandrin, and deoxyschisandrin, which are major compounds in fruits of Schisandra chinensis, were the most abundant lignans in both samples. In the future, the composition of lignans in the omija wine and cheong may be of value for their dietary and nutritional application.
Information on the levels of seven lignans in omija wine and omija cheong is presented in Table 2. Typical SRM chromatograms obtained in the analysis of seven lignans in omija wine and omija cheong are illustrated in Figure 3. Omija wine and cheong contained much higher levels of gomisin A and schisandrin, which are index components of omija specified in the Korean Pharmacopoeia¹⁶ than other lignans. Gomisin A has been known to reduce metastatic melanoma and colorectal cancer through its antiproliferative and anti-metastatic activities.

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