[논문]A New Report of Biscogniauxia petrensis Isolated from Mosquitoes in Korea

Das, Kallol; Kim, Joung-Ho; Choi, Kwang-Shik; Lee, Seung-Yeol; Jung, Hee-Young

doi:10.4489/kjm.20200010

A New Report of Biscogniauxia petrensis Isolated from Mosquitoes in Korea 원문보기

한국균학회지 = The Korean journal of mycology, v.48 no.2, 2020년, pp.87 - 93

Das, Kallol (School of Applied Biosciences, Kyungpook National University) , Kim, Joung-Ho (School of Applied Biosciences, Kyungpook National University) , Choi, Kwang-Shik (School of Life Sciences, Kyungpook National University) , Lee, Seung-Yeol (School of Applied Biosciences, Kyungpook National University) , Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University)

Abstract ▼ AI-Helper

A fungal strain designated KNU-WDM2A2 was isolated from mosquitoes in Gimcheon, Korea. The pure culture was transferred to potato dextrose agar (PDA) and synthetic nutrient agar (SNA) media and attained a diameter of 90 mm after 10 days of incubation at 25℃. The colonies were whitish to light pink and cottony to wooly, with an abundant production of aerial mycelia. The strain produced hyaline to slightly yellowish conidiophores that were rough-walled and branched, with conidiogenous cells arising terminally or laterally. Conidia were unicellular, hyaline to light brown, smooth, and oval or ovoid to clavate, with a size of 4.1-6.9×2.5-3.3 ㎛ (n=65). A phylogenetic analysis was conducted using the internal transcribed spacer (ITS) regions and 28S rDNA of large subunit (LSU) sequences, to support the cultural and morphological characteristics. The KNU-WDM2A2 strain was identified here as Biscogniauxia petrensis, new to Korea.

주제어

표/그림 (4)

표 Table 1. List of species used in phylogenetic analyses with GenBank accession numbers
그림 Fig. 1. Cultural and morphological characteristics of Biscogniauxia petrensis KNU-WDM2A2.
표 Table 2. Morphological characteristics of the KNU-WDM2A2 strain with reference to Biscogniauxia petrensis
그림 Fig. 2. Neighbor-joining phylogenetic tree based on the combined internal transcribed spacer (ITS) regions and the partial sequence of 28S rDNA genes, showing the relationships of Biscogniauxia petrensis.

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

During the screening of fungal species in Korea, the KNU-WDM2A2 strain was isolated from mosquitoes and identified as Biscogniauxia petrensis, which is a fungal species that had not been reported in Korea. In this study, molecular phylogenetic analyses were used to identify the undescribed fungal species, and its cultural and morphological characteristics were examined.
2 g/L of sucrose) [9] were used to study the cultural and morphological characteristics of the KNU-WDM2A2 strain after incubation at 25℃ for 14 days [10]. Subsequently, the cultural characteristics, such as colony color, fungal growth, and texture, were recorded, and the morphology of the fungus was observed using a light microscope (BX-50; Olympus, Tokyo, Japan).
The HiGene Genomic DNA prep kit (BIOFACT, Daejeon, Korea) was used as per the manufacturer s instructions. The PCR amplification process was carried out on the internal transcribed spacer (ITS) regions using the primer pairs ITS1F/ITS4 [11,12] and the 28S rDNA large subunit (LSU) with LROR/LR5 [13]. Finally, the amplified PCR products were purified using EXOSAP-IT (Thermo Fisher Scientific, Waltham, MA, USA) and sequenced by Solgent Co.

데이터처리

Evolutionary distance matrices were generated based on neighbor-joining algorithm with the Kimura model [14]. The phylogenetic analyses were performed using the MEGA 7.0 software with bootstrap analysis of 1,000 replications [15].

이론/모형

petrensis (KU746717, KU746716, KU746715, MK951680). A phylogenetic tree was constructed based on the combined sequences of ITS regions and partial sequences of the 28S rDNA using a neighbor-joining method. The generated phylogenetic tree revealed that the KNU-WDM2A2 strain was clustered with the previously identified B.
To perform the phylogenetic analysis, the consensus sequences were retrieved from the GenBank database of the National Center for Biotechnology Information (NCBI) (Table 1). Evolutionary distance matrices were generated based on neighbor-joining algorithm with the Kimura model [14]. The phylogenetic analyses were performed using the MEGA 7.

성능/효과

2. Neighbor-joining phylogenetic tree based on the combined internal transcribed spacer (ITS) regions and the partial sequence of 28S rDNA genes, showing the relationships of Biscogniauxia petrensis. The numbers above the branches represent the bootstrap values obtained for 1,000 replicates.
The BLAST results of the ITS sequence exhibited a similarity of 100% with different strains of Biscogniauxia petrensis (MN844525, MN844517, MN844529, MN844513, and MN844542). The 28S rDNA of the large subunit displayed maximum similarity of 99.88% with the different strains of B. petrensis (KU746717, KU746716, KU746715, MK951680). A phylogenetic tree was constructed based on the combined sequences of ITS regions and partial sequences of the 28S rDNA using a neighbor-joining method.
After the analysis of nucleotide sequences, the sequences of 686 and 846 bp were obtained from the ITS regions and 28S rDNA, respectively. The BLAST results of the ITS sequence exhibited a similarity of 100% with different strains of Biscogniauxia petrensis (MN844525, MN844517, MN844529, MN844513, and MN844542). The 28S rDNA of the large subunit displayed maximum similarity of 99.

후속연구

In this study, KNU-WDM2A2 strain was isolated from adult mosquitoes and identified as Biscogniauxia petrensis in Korea. Therefore, further research is required to provide in-depth knowledge about this species based on ecology and aspects of the agricultural field in Korea.

참고문헌 (24)

Hyde KD, Jones EBG, Liu JK, Ariyawansa H, Boehm E, Boonmee S, Braun U, Chomnunti P, Crous PW, Dai DQ, et al. Families of Dothideomycetes. Fungal Divers 2013;63:1-313.
Maharachchikumbura SSN, Hyde KD, Jones EBG, McKenzie EHC, Bhat JD, Dayarathne MC, Huang SK, Norphanphoun C, Senanayake IC, Perera RH, et al. Families of Sordariomycetes. Fungal Divers 2016;79:1-317.
U’Ren JM, Miadlikowska J, Zimmerman NB, Lutzoni F, Stajich JE, Arnold AE. Contributions of North American endophytes to the phylogeny, ecology, and taxonomy of Xylariaceae (Sordariomycetes, Ascomycota). Mol Phylogenet Evol 2016;98:210-32.

상세보기
Fournier J, Lechat C, Courtecuisse R. The genus Biscogniauxia (Xylariaceae) in Guadeloupe and Martinique (French West Indies). Ascomyceteorg 2017;9:67-99.
Hsieh HM, Ju YM, Rogers JD. Molecular phylogeny of Hypoxylon and closely related genera. Mycologia 2005;97:844-65.

상세보기
Daranagama DA, Camporesi E, Tian Q, Liu X, Chamyuang S, Stadler M, Hyde KD. Anthostomella is polyphyletic comprising several genera in Xylariaceae. Fungal Divers 2015;73:203-38.
Senanayake IC, Maharachchikumbura SSN, Hyde KD, Bhat JD, Jones EBG, McKenzie EHC, Dai DQ, Daranagama DA, Dayarathne MC, Goonasekara ID, et al. Towards unraveling relationships in Xylariomycetidae (Sordariomycetes). Fungal Divers 2015;73:73-144.
Wendt L, Sir EB, Kuhnert E, Heitkamper S, Lambert C, Hladki AI, Romero AI, Luangsaard JJ, Srikitikulchai P, Persoh D, et al. Resurrection and emendation of the Hypoxylaceae, recognised from a multigene genealogy of the Xylariales. Mycol Prog 2017;17:115-54.
Nirenberg HI, Aoki T. Fusarium nisikadoi, a new species from Japan. Mycoscience 1997;38:329-33.

상세보기
Zhang ZF, Liu F, Zhou X, Liu XZ, Liu SJ, Cai L. Culturable mycobiota from Karst caves in China, with descriptions of 20 new species. Persoonia 2017;39:1-31.

상세보기
Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8.

상세보기
White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR protocols: a guide to methods and applications. New York: Academic Press; 1990. p. 315-22.
Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 1990;172:4238-46.

상세보기
Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111-20.

상세보기
Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870-4.

상세보기
Pereira EDS, Sarquis MIDM, Ferreira-Keppler RL, Hamada N, Alencar YB. Filamentous fungi associated with mosquito larvae (Diptera: Culicidae) in Municipalities of the Brazilian Amazon. Neotrop Entomol 2009;38:352-9.
Da Costa GL, De Oliveira PC. Penicillium species in mosquitoes from two Brazilian regions. J Basic Microbiol 1998;38:343-7.

상세보기
Heo I, Hong K, Yang H, Lee HB, Choi YJ, Hong SB. Diversity of Aspergillus, Penicillium, and Talaromyces species isolated from freshwater environments in Korea. Mycobiology 2019;47:12-9.

원문보기 상세보기
Nguyen TTT, Lee HB. Isolation and characterization of Blakeslea trispora isolated from gut of grasshopper and soldier fly larva in Korea. Kor J Mycol 2016;44:355-9.
Nguyen TTT, Duong TT, Lee HB. Characterization of two new records of Mucoralean species isolated from gut of soldier fly larva in Korea. Mycobiology 2016;44:310-3.

원문보기 상세보기
Inacio ML, Henriques J, Guimaraes GL, Azinheira HG, Lima A, Sousa E. Platypus cylindrus Fab. (Coleoptera: Platypodidae) transports Biscogniauxia mediterranea, agent of cork oak charcoal canker. Bol San Veg Plagas 2011;37:181-6.
Hyde KD, Norphanphoun C, Maharachchikumbura SSN, Bhat DJ, Jones EBG, Bundhun D, Chen YJ, Bao DF, Boonmee S, Calabon MS, et al. Refined families of Sordariomycetes. Mycosphere 2020;11:305-1059.
Raimondo ML, Lops F, Carlucci A. Charcoal canker of pear, plum, and quince trees caused by Biscogniauxia rosacearum sp. nov. in southern Italy. Plant Dis 2016;100:1813-22.

상세보기
Luchi1 N, Capretti P, Vettraino AM, Vannini A, Pinzani P, Pazzagli M. Early detection of Biscogniauxia nummularia in symptomless European beech (Fagus sylvatica L.) by TaqManTM quantitative real-time PCR. Lett Appl Microbiol 2006;43:33-8.

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증