Ku, Hye-Jin
(Department of Food Science and Biotechnology, Graduate School of Biotechnology, Kyung Hee University)
,
Kim, You-Tae
(Department of Food Science and Biotechnology, Graduate School of Biotechnology, Kyung Hee University)
,
Lee, Ju-Hoon
(Department of Food Science and Biotechnology, Graduate School of Biotechnology, Kyung Hee University)
To understand the formation of initial gut microbiota, three initial fecal samples were collected from two groups of two breast milk-fed (BM1) and seven formula milk-fed (FM1) infants, and the compositional changes in gut microbiota were determined using metagenomics. Compositional change analysis d...
To understand the formation of initial gut microbiota, three initial fecal samples were collected from two groups of two breast milk-fed (BM1) and seven formula milk-fed (FM1) infants, and the compositional changes in gut microbiota were determined using metagenomics. Compositional change analysis during week one showed that Bifidobacterium increased from the first to the third fecal samples in the BM1 group (1.3% to 35.1%), while Klebsiella and Serratia were detected in the third fecal sample of the FM1 group (4.4% and 34.2%, respectively), suggesting the beneficial effect of breast milk intake. To further understand the compositional changes during progression from infancy to childhood (i.e., from three weeks to five years of age), additional fecal samples were collected from four groups of two breast milk-fed infants (BM2), one formula milk-fed toddler (FM2), three weaning food-fed toddlers (WF), and three solid food-fed children (SF). Subsequent compositional change analysis and principal coordinates analysis (PCoA) revealed that the composition of the gut microbiota changed from an infant-like composition to an adult-like one in conjunction with dietary changes. Interestingly, overall gut microbiota composition analyses during the period of progression from infancy to childhood suggested increasing complexity of gut microbiota as well as emergence of a new species of bacteria capable of digesting complex carbohydrates in WF and SF groups, substantiating that diet type is a key factor in determining the composition of gut microbiota. Consequently, this study may be useful as a guide to understanding the development of initial gut microbiota based on diet.
To understand the formation of initial gut microbiota, three initial fecal samples were collected from two groups of two breast milk-fed (BM1) and seven formula milk-fed (FM1) infants, and the compositional changes in gut microbiota were determined using metagenomics. Compositional change analysis during week one showed that Bifidobacterium increased from the first to the third fecal samples in the BM1 group (1.3% to 35.1%), while Klebsiella and Serratia were detected in the third fecal sample of the FM1 group (4.4% and 34.2%, respectively), suggesting the beneficial effect of breast milk intake. To further understand the compositional changes during progression from infancy to childhood (i.e., from three weeks to five years of age), additional fecal samples were collected from four groups of two breast milk-fed infants (BM2), one formula milk-fed toddler (FM2), three weaning food-fed toddlers (WF), and three solid food-fed children (SF). Subsequent compositional change analysis and principal coordinates analysis (PCoA) revealed that the composition of the gut microbiota changed from an infant-like composition to an adult-like one in conjunction with dietary changes. Interestingly, overall gut microbiota composition analyses during the period of progression from infancy to childhood suggested increasing complexity of gut microbiota as well as emergence of a new species of bacteria capable of digesting complex carbohydrates in WF and SF groups, substantiating that diet type is a key factor in determining the composition of gut microbiota. Consequently, this study may be useful as a guide to understanding the development of initial gut microbiota based on diet.
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가설 설정
(A) The relative abundance of each feeding type. (B) Compositional gut microbiota change in 15 major bacterial taxa by dietary change. Closed circle with a solid line, BM; closed square with dashed line, FM.
제안 방법
2. Compositional change in gut microbiota from three weeks to five years of age for evaluating the impact of diet and individual variations. (A) The relative abundance of the taxonomic distributions in each individual sample.
대상 데이터
Consequently, this study revealed the initiation and development of gut microbiota during progression from infancy to five years of age in Korean children. Therefore, it may provide a basis for a more comprehensive understanding of the maturation of the Korean initial gut microbiota and the role of the gut microbiota in lifelong health.
후속연구
Consequently, this study revealed the initiation and development of gut microbiota during progression from infancy to five years of age in Korean children. Therefore, it may provide a basis for a more comprehensive understanding of the maturation of the Korean initial gut microbiota and the role of the gut microbiota in lifelong health.
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