[논문]쿠니츠트립인히비터, 렉틴 및 7S α' 서버유닛 3가지 단백질이 없는 콩 계통의 개발

채원기; 최상우; 강경영; 정종일

doi:10.5352/jls.2020.30.7.592

쿠니츠트립인히비터, 렉틴 및 7S α' 서버유닛 3가지 단백질이 없는 콩 계통의 개발
The Development of a New Soybean Strain Without Kunitz Trypsin Inhibitor, Lectin, and 7S α' Subunit Protein 원문보기

생명과학회지 = Journal of life science, v.30 no.7, 2020년, pp.592 - 597

채원기 (경상대학교 농학과) , 최상우 (경상대학교 농학과) , 강경영 (경상대학교 농학과) , 정종일 (경상대학교 농학과)

초록
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성숙 콩[Glycine max (L.) Merr.] 종자는 약 40%의 단백질을 함유하고 있으며 아이소플라본, 사포닌, 루테인, 비타민등 다양한 기능성 성분을 함유하고 있다. 그러나, Kunitz Trypsin Inhibitor (KTI) 단백질, 렉틴 단백질, 7S α' 서버유닛 단백질이 함유되어져 있어 콩의 품질과 기능성을 저하시키고 있다. 본 연구는 콩 및 콩 제품의 품질과 기능성을 저하시키는 KTI, 렉틴 및 7S α' 서버유닛 3가지 단백질이 모두 유전적으로 결핍된 콩 계통(titilelecgy1cgy1 유전자형)을 선발하기 위하여 진행되었다. 3개의 모본(개척2호, PI506876, Le-16)을 이용한 유전집단으로부터 성숙 종자에서 KTI, 렉틴 및 7S α' 서버유닛 3가지 단백질이 모두 없는 titilelecgy1cgy1 유전자형을 가진 1개의 계통을 개발하였다. 개발된 계통은 자주색 꽃, 유한신육형, 노란종피을 가지고 있으며 초장은 58 cm로 대원콩(46 cm)보다 길었다. 백립중은 27.1 g으로 대원콩(29.0 g)보다 작았다. 본 연구를 통하여 선발된 계통은 KTI, 렉틴 및 7S α' 서버유닛 3가지 단백질이 모두 없는 고품질 기능성 콩 품종 육성을 위한 중간모본으로 이용될 수 있을 것으로 사료되었다.

Abstract ▼ AI-Helper

Soybean [Glycine max (L.) Merr.] seeds contain an average of 40% protein on a dry weight basis, but they also contain antinutritional elements such as lectin, Kunitz trypsin inhibitor (KTI), and 7S α'- subunit protein. The objective of this research was to develop a new soybean genotype with triple recessive alleles for these elements. Three parents (Gaechuck#2, PI506876, and Le-16) were used to develop the genetic population, and the presence of lectin and KTI protein was detected using Western blot while 7S α' subunit protein was detected using SDS-PAGE. One F3 plant strain with proper agronomical traits such as type, height, seed quality, and 100-seed weight was selected. The genotype of the developed strain is titilelecgy1cgy1, that is KTI, lectin, and 7S α' subunit protein free. The new strain has a purple flower, determinate growth habit, and light yellow pods at maturity. The seed has a buffer hilum and is yellow in color. The new strain's height was 58 cm compared to the Daewonkong cultivar at 46 cm, and its 100-seed weight was 27.1 g, smaller than the Daewonkong at 29.0 g. This is the first new soybean strain with the titilelecgy1cgy1 genotype, and it can be used to improve yellow soybean cultivars of high quality and function.

주제어

표/그림 (5)

표 Table 1. Genotype for Ti (ti), Le (le), and Cgy1 (cgy1) alleles of parents used in this experiment
그림 Fig. 1. Scheme for development of soybean strain with titilelecgy1cgy1 genotype (KTI protein free, lectin protein free and 7S α' subunit protein free).
그림 Fig. 2. Confirmation of Kunitz Trypsin Inhibitor (KTI) protein free (A), lectin protein free (B), and 7S α' subunit protein free (C) in the current cultivar ("Daewonkong") and new strain.
그림 Fig. 3. Plant (A) and seed (B) of new soybean strain (titilelecgy1cgy1 genotype) with Kunitz Trypsin Inhibitor protein free, lectin protein free, and 7S α' subunit protein free.
표 Table 2. Agronomic traits of cultivar and new strain developed in this experiment

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문제 정의

The objective of this research was to develop new soybean genotype with triple recessive alleles (titilelecgy1cgy1) for KTI protein, lectin protein and 7S α' subunit protein.

가설 설정

F₃ seeds with titilelecgy1cgy1 genotype were planted to F₃ plant generation. F₃ plants with a proper agronomic traits were individually harvested. Random F₄ seeds from F₃ plants harvested were used to confirm KTI protein free, lectin protein free, and 7S α' subunit protein free.

대상 데이터

Four F2 seeds with titilelecgy1cgy1 genotype (KTI, lectin and 7S α' subunit protein free) were selected and were planted.

데이터처리

Mean values of plant height and 100-seed weight were compared by Duncan’s multiple range test at the 5% level.

성능/효과

Four F₂ plants were individually harvested and two F₂ plants with a proper agronomic traits were selected. Two F₃ seed strains were planted and one F₃ plant strain with a proper agronomical traits such as plant type, plant height, seed quality, and 100-seed weight was finally selected. Random F₄ seeds were used to confirm absence for KTI, lectin and 7S α' subunit proteins (Fig.

참고문헌 (22)

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상세보기
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상세보기
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상세보기
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