[논문]유용 홍조류 참풀가사리(Gloiopeltis tenax) 반상근의 생장에 미치는 온도와 광도의 영향

이현정; 김영식

doi:10.23005/ksmls.2020.5.2.64

유용 홍조류 참풀가사리(Gloiopeltis tenax) 반상근의 생장에 미치는 온도와 광도의 영향
Effects of Temperature and Irradiance on the Growth of Basal Crust of Economic Red Alga Gloiopeltis tenax 원문보기

한국해양생명과학회지 = Journal of marine life science, v.5 no.2, 2020년, pp.64 - 71

이현정 (국립생태원 생태공간연구팀) , 김영식 (국립군산대학교 해양생명응용과학부)

초록
AI-Helper

홍조류 참풀가사리(Gloiopeltis tenax)는 중국과 일본에서 경제적으로 유용한 종으로 알려져 있으며 이것은 예로부터 접착제나 식용 등으로 이용되었다. 실험에 사용된 재료는 전북 부안군 변산면 격포리에서 채집한 성숙한 사분포자체로부터 사분포자를 방출 받은 후 배양하여 형성된 반상근을 대량으로 확보하였다. 이를 해부현미경 하에서 4등분하여 재생과 생장 과정을 추적하였다. 절단된 반상근을 각 조건별로 배양하였고 광주기는 16:8 h L:D이며 온도조건은 10, 15, 20, 25℃이고 광도조건은 30, 85 µmol photons m^-2s^-1이다. 4등분된 반상근은 타원형으로 생장하다가 원주상의 직립 축을 형성하였다. 반상근의 크기는 배양 12주 후, 광도 85 µmol photons m^-2s^-1의 15℃ 온도에서 9.61±3.59 mm²로 최대였다. 평균 상대생장률은 광도 85 µmol photons m^-2s^-1의 15℃ 온도에서 5.15±0.80 %day-1으로 최대 생장률을 나타내었고 10℃ 온도에서 3.15±0.94 %day^-1으로 최저 생장률을 나타냈으며 통계적으로 유의한 차이를 보였다(p < 0.05). 결론적으로 참풀가사리의 반상근을 이용한 방법이 효과적인 양식 방법임이 확인되었다.

Abstract ▼ AI-Helper

Gloiopeltis tenax is one of the most economically useful species in China and Japan that has been applied to glue and food since ancient times. The material used in the experiment was a large quantity of basal crusts obtained from the culture of tetraspores that were released from the mature tetrasporophytes collected at Gyeokpori, Byeonsan-myeon, Buan-gun, Jeollabuk-do in Korea. The basal crusts were cut into quadrisect under a dissecting microscope so as to monitor the process of regeneration and growth. The cut crusts were cultured under varying conditions, where the photoperiod was 16:8 h L:D; the temperature range was 10, 15, 20, and 25℃; the irradiance range was 30 and 85 µmol photons m-2s-1. The quadrisect basal crusts grew to an oval shape, then formed a vertical axis of cylindrical shape. The maximum growth of basal crusts was 9.61±3.59 mm2 under the condition of 15℃ temperature and 85 µmol photons m-2s-1 irradiance, after 12-weeks culture. The mean relative growth rate after 12-weeks culture showed the maximum rate of 5.15±0.80 %day-1 at 15℃ and the minimum growth rate was 3.15±0.94 %day-1 at 10℃, with a statistically significant difference (p < 0.05). In conclusion, it has shown the possibility that growth of basal crusts of G. tenax is one of the good farming method of potential alga.

주제어

표/그림 (6)

그림 Fig. 1. Development of Gloiopeltis tenax in culture. (A) Tetrasporophytic plant; (B) Cross-section of tetrasporophytic plant (arrowheads are tetraspores); (C-D) Spores developed to the discoid crusts; (E), Cut fragments of basal crusts; (F) 6-month cultivated basal crust. Scale bars represent: A, 2 cm; B-D 20 μm; E, 200 μm; F, 1 cm.
그림 Fig. 2. Growth of crust (area) of Gloiopeltis tenax cultured for 12 weeks under 34 psu, 16:8 h L:D and two different irradiances conditions (n=3 replicates). Vertical bars indicate SD.
표 Table 1. RGR (%day^-1) of Gloiopeltis tenax cultured for 12 weeks under 34 psu, 16:8 h L:D, and two different irradiances conditions. Values are means ± SE (n=3 replicates). unit, mm
그림 Fig. 3. Percentage of sprouting dis (%) of Gloiopeltis tenax in culture. (A) 30 μmol photons m^-2s^-1, (B) 85 μmol photons m^-2s^-1.
표 Table 2. Average RGR (%day^-1) of Gloiopeltis tenax for 12 weeks. Culture conditions are 34 psu, and 16:8 h L:D. Values (means ± SD, n=3replicates) in each row with the different superscripts are significantly different (p<0.05)
표 Table 3. Comparison of maximum RGR (%day^-1) for the optimal condition in each study

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