[논문]Isolation and characterization of unrecorded yeasts species in the family Metschnikowiaceae and Bulleribasidiaceae in Korea

Park, Yuna; Maeng, Soohyun; Srinivasan, Sathiyaraj

doi:10.12651/jsr.2020.9.3.198

Isolation and characterization of unrecorded yeasts species in the family Metschnikowiaceae and Bulleribasidiaceae in Korea 원문보기

Journal of species research, v.9 no.3, 2020년, pp.198 - 203

Park, Yuna (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) , Maeng, Soohyun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) , Srinivasan, Sathiyaraj (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)

Abstract ▼ AI-Helper

The goal of this study was to isolate and identify wild yeasts from soil samples. The 15 wild yeast strains were isolated from the soil samples collected in Pocheon city, Gyeonggi Province, Korea. Among them, four yeast stains were unrecorded, and 11 yeast stains were previously recorded in Korea. To identify wild yeasts, microbiological characteristics were observed by API 20C AUX kit. Pairwise sequence comparisons of the D1/D2 domain of the 26S rRNA were performed using Basic Local Alignment Search Tool(BLAST). Cell morphology of yeast strains was examined by phase contrast microscope. All strains were oval-shaped and polar budding and positive for assimilation of glucose, 2-keto-ᴅ-gluconate, N-acetyl-ᴅ-glucosamine, ᴅ-maltose and ᴅ-saccharose (sucrose). There is no official report that describes these four yeast species: one strain of the genus Kodamaea in the family Metschnikowiaceae and three strains of the Hannaella in the family Bulleribasidiaceae. Kodamaea ohmeri YI7, Hannaella kunmingensis YP355, Hannaella luteola YP230 and Hannaella oryzae YP366 were recorded in Korea, for the first time.

주제어

표/그림 (5)

그림 Fig. 1. Photomicrographs showing budding cells of strains YI7 (a); YP355 (b); YP366 (c); YP320 (d).
표 Table 1. Yeasts isolated from soils of Korea.
그림 Fig. 2. Phylogenetic tree derived from neighbor-joining analysis based on the D1/D2 domains of the LSU rRNA gene sequences, showing the placement of strain YI7 in the Kodamaea clade.
그림 Fig. 3. Phylogenetic tree derived from neighbor-joining analysis based on the D1/D2 domains of the LSU rRNA gene sequences, showing the placement of strains YP355, YP230 and YP366 in the genus Hannaella.
표 Table 2. Characteristics of the unrecorded yeasts from soil in Korea.

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문제 정의

This study focuses on the description of four yeast species belonging to genera Kodamaea and Hannaella that have not officially been reported in Korea.
The identified yeasts belong to the family Metschnikowiaceae and Bulleribasidiceae. This study focusses on the isolation and description of unrecorded species in the genera Kodamaea and Hannaella.

대상 데이터

Soil samples were collected in Pocheon city, Gyeonggi Province, Korea and serially diluted in distilled water. The aliquot was spread onto YM agar incubated at 25℃ for 3-4 days.
In the API 20C AUX, strain YI7 is positive for assimilation of l-arabinose (w, weak), adonitol(w), D_^-lactose (bovine origin)(w), D-melezitose (w), glucose, glycerol, 2-keto-D-gluconate, D-galactose, D-sorbitol, D-methyl-D-glucoside, N-acetyl-D-glucosamine, D-cellobiose, D-maltose, D-saccharose (sucrose), D-trehalose and D-raffinose; but negative for D-xylose, xylitol and inositol. Strain YI7 was isolated from a soil sample collected in Pocheon city, Gyeonggi Province, Korea.
In the API 20C AUX, strain YP230 is positive for assimilation of glycerol (w), adonitol (w), xylitol (w), D-sorbitol (w), d-methyl-D-glucoside (w), D-lactose (bovine origin) (w), glucose, 2-keto-D-gluconate, D-galactose, inositol, N-acetyl-D-glucosamine, D-cellobiose, D-maltose, D-saccharose (sucrose), D-trehalose, D-melezitose and D-raffinose; but negative for _L-arabinose and D-xylose. Strain YP230 was isolated from a soil sample collected in Pocheon city, Gyeonggi Province, Korea.
In the API 20C AUX, strain YP335 is positive for assimilation of l-arabinose (w), D-xylose (w), D-galactose (w), D-sorbitol (w), D-cellobiose (w), D-raffinose (w), glucose, 2-keto-d-gluconate, N-acetyl-D-glucosamine, D-maltose, d-saccharose (sucrose) and D-melezitose; but negative for glycerol, adonitol, xylitol, inositol, D-methyl-d-glucoside, d-lactose (bovine origin) and D-trehalose. Strain YP335 was isolated from a soil sample collected in Pocheon city Gyeonggi Province, Korea.
In the API 20C AUX, strain YP366 is positive for assimilation of D-cellobiose (w), glucose, 2-keto-D-gluconate, _L-arabinose, D-xylose, D-galactose, inositol, D-methyl-D-glucoside, N-acetyl-D-glucosamine, D-maltose, D-saccharose (sucrose), D-melezitose and D-raffinose; but negative for glycerol, adonitol, xylitol, D-sorbitol, D-lactose (bovine origin) and D-trehalose. Strain YP366 was isolated from a soil sample collected in Pocheon city, Gyeonggi Province, Korea.

이론/모형

Biochemical characteristics were established using API 20C AUX (bioMérieux) according to the manufacturer’s instructions.
0 (Saitou and Nei, 1987). The evolutionary distances were calculated using the two-parameter model of Kimura (Kimura, 1980) for the neighbor-joining analyses. The confidence levels of the clades were estimated through bootstrap analysis (1,000 replicates)(Felsenstein, 1985).

성능/효과

The strains YI, YP335, YP230 and YP366 were most closely related to K. ohmeri (CBS5367^T; 99% D1/D2 domain of LSU sequence similarity), H. kunmingensis (CBS8960^T; 100%), H. luteola (CBS943^T; 100%) and H. oryzae (CBS7194^T; 99%), respectively. The D1/D2 domain of the LSU rRNA gene similarities of the four strains also formed a robust phylogenetic clade with the most closely related species(Figs.

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