[논문]Association of PLIN2 polymorphisms with economic traits in Berkshire pigs

Kim, Yesong; Seong, Jiyeon; Lee, Yoonseok; Kong, Hong Sik

doi:10.12750/jarb.35.3.239

[국내논문] Association of PLIN2 polymorphisms with economic traits in Berkshire pigs 원문보기

Journal of animal reproduction and biotechnology = 한국동물생명공학회지, v.35 no.3, 2020년, pp.239 - 244

Kim, Yesong (The Graduate School, Department of Animal Life and Environment Science, Hankyong National University) , Seong, Jiyeon (Genomic Informatics Center, Hankyong National University) , Lee, Yoonseok (Genomic Informatics Center, Hankyong National University) , Kong, Hong Sik (The Graduate School, Department of Animal Life and Environment Science, Hankyong National University)

Abstract ▼ AI-Helper

Meat and carcass quality attributes are important factors influencing consumer preference and profitability in the pork industry. Single nucleotide polymorphisms (SNPs) are essential for livestock breeding and improvement. In the present study, the pig Perilipin 2 (PLIN2) gene was characterized with the aim of detecting genetic variation at these loci in relation to economic traits in Berkshire pigs. Four SNPs (G6714C, G6813A, G10340A, and G10632A) were detected in this studied. Statistical analysis indicated that G6714C was significantly associated with the National Pork Producers Council (NPPC) color score, G6813A, and G10340A significantly affected NPPC color score and NPPC marbling score, and G10632A significantly affected backfat thickness (BF) (p < 0.05). Therefore, the molecular markers used in the present study might provide a useful basis for identification and improvement of traits in the Berkshire pigs.

주제어

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문제 정의

In this study, the efficiency of PLIN2 polymorphisms for Berkshire pigs economic traits was examined and suggested that polymorphisms might be important genetic factors influencing economic traits in Berkshire breeds.
The purpose of this study was to find a molecular marker for the improved economic traits in pig using candidate gene analysis. We identified four SNPs (G6714C, G6813A, G10340A, and G10632A) in the Berkshire pig PLIN2 gene.

제안 방법

Slaughtering followed standard procedures under the supervision of a Korean grading service for animal products. After slaughter, 103 pigs were used for the analysis and a total of 13 traits including: carcass weight (CW), backfat thickness (BF), pH value after 45 minutes (pH45m), pH value after 24 hours (pH24h), meat color L, a, b, filter-paper fluid uptake (FFU), drip loss (DL), cooking loss (CL), muscle shear force (SF) national pork producers council (NPPC) color score and NPPC marbling score. Following slaughter, the CW was recorded and the BF was measured between the 10th and 11th ribs.
To investigate the effects of SNPs, the association of the PLIN2 genotypes were analyzed to determine the effects on variation in the economic traits in Berkshire pigs. Statistical analysis indicated that G6714C was significantly associated with the NPPC color score, G6813A, and G10340A significantly affected NPPC color score and NPPC marbling score, and G10632A significantly affected BF (p < 0.

대상 데이터

A total of 103 Berkshire pigs (41 sows and 62 boars) were used for the association study. Meat quality traits were studied.

데이터처리

Statistical analysis of the association of the genotypes based on the molecular markers with the economic traits was evaluated with a general linear model (GLM) fit by the least squares method using the SAS software package (SAS institute, USA). The GLM used in the present study was

성능/효과

The GA (3.73 ± 0.10, 3.75 ± 0.10) genotype had a higher NPPC color score than that in the AA (3.40 ± 0.25, 3.41 ± 0.26) and GG genotype (3.38 ± 0.07, 3.36 ± 0.07); GG (2.46 ± 0.236, 2.46 ± 0.233) genotype had a higher NPPC marbling score than that in AA (2.08± 0.0.065, 2.07 ± 0.065) and GA (1.94 ± 0.092, 1.91 ±0.092) genotype.
Statistical analysis indicated that G6714C was significantly associated with the NPPC color score, G6813A, and G10340A significantly affected NPPC color score and NPPC marbling score, and G10632A significantly affected BF (p < 0.05).
We identified four SNPs in Berkshire pigs PLIN2 gene; one SNP (G6714C) was detected in the intron 5 region, one SNP (G6813A) was detected in the exon 6 region, one SNP (G10340A) was detected in the exon 8 region, and one SNP (G10632A) was detected 3’UTR region.
We identified four SNPs (G6714C, G6813A, G10340A, and G10632A) in the Berkshire pig PLIN2 gene. The G6714C was significantly associated with the NPPC color score, G6813A, and G10340A significantly affected NPPC color score and NPPC marbling score, and G10632A significantly affects BF (p < 0.05). These results indicate that PLIN2 may be candidate genes for economic traits.
The SNPs G6714C was associated with a significant effect on NPPC color score, the GC (3.80 ± 0.12) genotype had a higher NPPC color score than that in the CC (3.28 ± 0.25) and GG (3.15 ± 0.06) genotype (p < 0.05).

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