토마토(Lycopersicon esculentum)로부터 flavone 화합물의 분리 동정과 세포 내 GSH 회복능을 통한 항산화 활성 평가 Flavone from the Lycopersicon esculentum and their antioxidant capacity through GSH recovery effect원문보기
토마토 액상을 70% MeOH 수용액으로 추출하고, 얻어진 추출물을 EtOAc n-BuOH 및 물로 용매 분획 하였다. 이 중 활성이 확인된 ethyl acetate (EtOAc) 분획으로부터 silica gel(SiO2)과 octadecyl silica gel column chromatography로 정제하여 1종의 화합물을 분리 하였다. Nuclear magnetic resornance, mass spectroscopy 및 infrarad spectroscopy 등의 스텍트럼 데이터를 통해 화합물 1의 화학구조를 5,7,3'-trihydroxy-6,4',5'-trimethoxyflavone (1) 로 동정 하였다. 화합물 1의 함량분석 결과, 추출물에서 0.96±0.03 ㎍/mg 그리고 EtOAc 분획(TME)에서 4.02±0.12 ㎍/mg으로 확인되었다. 본 연구를 통해서 glutathione mean의 증가와 glutathione heterogeneity의 감소를 보인 토마토 추출물 및 분획물과 토마토 유래 화합물이 세포 내 glutathione 수준을 균일하게 올려준다는 것을 입증함으로써 항산화 효능을 확인할 수 있었다.
토마토 액상을 70% MeOH 수용액으로 추출하고, 얻어진 추출물을 EtOAc n-BuOH 및 물로 용매 분획 하였다. 이 중 활성이 확인된 ethyl acetate (EtOAc) 분획으로부터 silica gel(SiO2)과 octadecyl silica gel column chromatography로 정제하여 1종의 화합물을 분리 하였다. Nuclear magnetic resornance, mass spectroscopy 및 infrarad spectroscopy 등의 스텍트럼 데이터를 통해 화합물 1의 화학구조를 5,7,3'-trihydroxy-6,4',5'-trimethoxyflavone (1) 로 동정 하였다. 화합물 1의 함량분석 결과, 추출물에서 0.96±0.03 ㎍/mg 그리고 EtOAc 분획(TME)에서 4.02±0.12 ㎍/mg으로 확인되었다. 본 연구를 통해서 glutathione mean의 증가와 glutathione heterogeneity의 감소를 보인 토마토 추출물 및 분획물과 토마토 유래 화합물이 세포 내 glutathione 수준을 균일하게 올려준다는 것을 입증함으로써 항산화 효능을 확인할 수 있었다.
The fruits of tomato (Lycopersicon esculentum) were extracted with 70% aqueous methanol (MeOH) and the concentrates were partitioned into ethyl acetate (EtOAc), n-butanol (n-BuOH), and water (H2O) fractions. The repeated silica gel (SiO2) and octadecyl silica gel column chromatographies for the EtOA...
The fruits of tomato (Lycopersicon esculentum) were extracted with 70% aqueous methanol (MeOH) and the concentrates were partitioned into ethyl acetate (EtOAc), n-butanol (n-BuOH), and water (H2O) fractions. The repeated silica gel (SiO2) and octadecyl silica gel column chromatographies for the EtOAc fraction, whose activity was confirmed, led to isolation of one flavone compound. Nuclear magnetic resornance, infrarad spectroscopy, and mass spectroscopy (MS) revealed the chemical structure of the isolated compound, 5,7,3'-trihydroxy-6,4',5'-trimethoxyflavone (1). LC-MS/MS analysis determined the content level of compounds 1 in the MeOH extract to be 4.02±0.12 ㎍/mg and in the TME-10 fraction to be 0.96±0.03 ㎍/mg. Through this study, the antioxidantive capacity was confirmed by demonstrating that the L. esculentum extract and their fractions showing an increase in glutathione mean and a decrease in glutathione heterogeneity uniformly raises the intracellular glutathione level.
The fruits of tomato (Lycopersicon esculentum) were extracted with 70% aqueous methanol (MeOH) and the concentrates were partitioned into ethyl acetate (EtOAc), n-butanol (n-BuOH), and water (H2O) fractions. The repeated silica gel (SiO2) and octadecyl silica gel column chromatographies for the EtOAc fraction, whose activity was confirmed, led to isolation of one flavone compound. Nuclear magnetic resornance, infrarad spectroscopy, and mass spectroscopy (MS) revealed the chemical structure of the isolated compound, 5,7,3'-trihydroxy-6,4',5'-trimethoxyflavone (1). LC-MS/MS analysis determined the content level of compounds 1 in the MeOH extract to be 4.02±0.12 ㎍/mg and in the TME-10 fraction to be 0.96±0.03 ㎍/mg. Through this study, the antioxidantive capacity was confirmed by demonstrating that the L. esculentum extract and their fractions showing an increase in glutathione mean and a decrease in glutathione heterogeneity uniformly raises the intracellular glutathione level.
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