[논문]수온과 저산소에 따른 조피볼락(Sebastes schlegeli)의 호흡대사와 혈액성상의 변화

김흥윤

doi:10.7847/jfp.2021.34.2.213

수온과 저산소에 따른 조피볼락(Sebastes schlegeli)의 호흡대사와 혈액성상의 변화
Changes in metabolic rate and hematological parameters of black rockfish (Sebastes schlegeli) in relation to temperature and hypoxia 원문보기

韓國魚病學會誌= Journal of fish pathology, v.34 no.2, 2021년, pp.213 - 224

김흥윤 (전남대학교 수산생명의학과)

초록
AI-Helper

본 연구는 수온 15, 20 및 25℃에서 조피볼락(Sebastes schlegeli)을 점진적인 저산소 조건에 노출시켜 호흡 대사율이 표준대사율(SMR) 이하로 감소하는 산소포화도, S_crit를 평가하고, 수온별 S_crit 에 4시간 노출 후 혈액 성상의 변화를 조사하기 위하여 실시하였다. 수온 15, 20, 25℃에서 normoxic SMR은 각각 116.5±5.5, 188.6±4.2 및 237.4±6.8 mg O₂/kg/hr이었고, 호흡계수(Q₁₀)는 15-20℃ 구간에서는 2.62, 20-25℃에서는 1.58, 그리고 전 수온 범위의 15-25℃에서는 2.04였다. 수온 15, 20 및 25℃에서 S_crit는 각각 22.1±1.2, 30.6±1.5 및 41.9±1.4 %였다. 수온별 S_crit에 4시간 노출 후 혈액 Ht와 Hb 및 혈장 cortisol, glucose, 전해질(Na⁺, K⁺, Cl^-)과 삼투질 농도는 모든 수온에서 normoxic water의 대조구에 비하여 유의하게 높았다. 수온 15, 20, 25℃의 normoxic water에서는 Ht, Hb 및 혈장 glucose 농도는 15℃에 비하여 20℃와 25℃에서, 혈장 cortisol, 전해질 및 삼투질 농도는 25℃에서 유의하게 높았다. 수온별 S_crit의 저산소에 노출된 조피볼락은 수온이 높을수록 저산소에 의한 어체의 생리적 스트레스는 가중되는 것으로 나타났다.

Abstract ▼ AI-Helper

Experiments were performed to investigate changes in metabolic rate (MO2), critical oxygen saturation (Scrit) and hematological parameters of black rockfish, Sebastes schlegeli exposed to hypoxia at 15, 20 and 25℃. The MO2 was measured at an interval of 10 min using intermittent-flow respirometry. The normoxic standard metabolic rate (SMR) was 116.5±5.5, 188.6±4.2 and 237.4±6.8 mg O2/kg/hr, and Scrit was 22.1±1.2, 30.6±1.5 and 41.9±1.4% air saturation at 15, 20 and 25℃, respectively. Q10 values were 2.62 between 15 and 20℃, 1.58 between 20 and 25℃, and 2.04 over the full temperature range. In the investigation of blood (hematocrit and hemoglobin) and biochemical parameters (plasma cortisol, glucose, electrolyte and osmolality), the rockfish were subjected to Scrit for each temperature during 4 hr. All of hematological parameters of the rockfish exposed to hypoxic water were significantly higher than those of normoxic control. Moreover, blood and biochemical parameters of the rockfish maintained to normoxic water showed the tendency of increase with temperature, and were significantly higher at 25C. As a result of this experiment, it was found that physiological stress due to hypoxia increased at high temperature.

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표/그림 (6)

그림 Fig. 1. Examples of metabolic rate (MO₂) data determined during 20 hr period and used to estimate the standard metabolic rate (SMR) and routine metabolic rate (RMR) in black rockfish Sebastes schlegeli at 15, 20 and 25℃, respectively. The total period taken for one determination of MO₂ was 10 min. Horizontal dotted lines indicate SMR for each fish calculated as the mean of the six (equals 1 hr) lowest measurements following the elevated MO₂ by initial handling. RMR was considered as higher measurements of MO₂ than the SMR. Numeric values are the fish's biomass.
표 Table 1. Standard metabolic rate (SMR), routine metabolic rate (RMR) and critical oxygen saturation (S_crit) in black rockfish Sebastes schlegeli exposed to normoxic and progressive hypoxic water at 15, 20 and 25℃, respectively
표 Table 2. Levels of plasma electrolyte and osmolality in black rockfish Sebastes schlegeli following 4 hr-exposure to normoxic and hypoxic water (critical oxygen saturation, S_crit for each temperature) at 15, 20 and 25℃, respectively
그림 Fig. 2. Critical oxygen saturation (S_crit) in black rockfish Sebastes schlegeli exposed to progressive hypoxic water at 15, 20 and 25℃, respectively. The horizontal dotted lines indicate SMR. Scrit was taken to be the intercept of the SMR and the regression line through the points below SMR. Data (showing opened-circles) include SMR and RMR measurements in Fig. 1. Numeric values are the fish's biomass.
그림 Fig. 3. Blood parameters in black rockfish Sebastes schlegeli following 4 hr-exposure to normoxic and hypoxic water (critical oxygen saturation, S_crit for each temperature). Experiments of normoxic and hypoxic exposure were performed separately. Data were analyzed via two-way ANOVA and Tukey's multiple range test. Data are presented as mean±S.E. (n=6). Bars with different letters are statistically different (P<0.05). Asterisks indicate significant difference between normoxic and hypoxic water.
그림 Fig. 4. Levels of plasma cortisol and glucose in black rockfish Sebastes schlegeli following 4 hr-exposure to normoxic and hypoxic water (critical oxygen saturation, S_crit for each temperature). Experiments of normoxic and hypoxic exposure were performed separately. Data were analyzed via two-way ANOVA and Tukey's multiple range test. Data are presented as mean±S.E. (n=6). Bars with different letters are statistically different (P<0.05). Asterisks indicate significant difference between normoxic and hypoxic water.

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