[논문]미역 추출물과 알긴산의 근육손실 억제 효능

최상윤; 김미나; 이현희; 허진영

doi:10.5352/jls.2021.31.2.137

미역 추출물과 알긴산의 근육손실 억제 효능
Undaria pinnatifida Extracts and Alginic Acid Attenuated Muscle Atrophy in TNF-α Induced Myoblast Cells through MAFbx Signaling Cascade 원문보기

생명과학회지 = Journal of life science, v.31 no.2, 2021년, pp.137 - 143

최상윤 (한국식품연구원 기능성소재연구단) , 김미나 (한국식품연구원 기능성소재연구단) , 이현희 (한국식품연구원 기능성소재연구단) , 허진영 (한국식품연구원 기능성소재연구단)

초록
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근육 위축증은 근섬유의 손상으로 인한 근육세포의 감소에 의해 일어나며 심장질환, 당뇨, 각종 노인성 만성질환을 일으킨다고 알려져 있다. 본 연구는 미역 추출물 및 이의 성분인 알긴산의 근육세포보호, 근육감소억제 및 근육재생효과를 확인하고자 하였다. 미역 추출물 및 알긴산을 분화된 C2C12 myoblast 세포에 처리한 결과 TNF-α에 의한 근육섬유의 감소가 억제하였고murf1과 MaFbx의 발현량도 감소하였다. 또한, 마우스에 미역 추출물 및 알긴산을 10주간 급여한 결과 cardiotoxin에 의한 다리부종이 감소하였으며 근육단백질인 MyHC와 PGC-1α의 발현량이 증가 하였다. 따라서 미역 추출물 및 알긴산은 근감소 억제 및 근육재생 효과를 나타내어 기능성소재의 활용 가능성을 확인하였다.

Abstract ▼ AI-Helper

Muscle atrophy refers to a decrease in muscle cells due to damage to muscle fibers. It is reported that muscle atrophy is caused by heart disease, diabetes, and other chronic diseases related to aging. The purpose of this study is to reveal the inhibitory effects of seaweed extracts, which are widely consumed in Korea, and alginic acid on muscle cell damage in muscle atrophy and regeneration models. We found that seaweed extracts (U) and alginic acid (A) attenuated TNF-α-induced muscle atrophy in differentiated C2C12 myoblast cells and inhibited muscle atrophy markers such as MuRF1 and MAFbx. In addition, U and A also regulated ubiquitination marker FoxO1 protein. To confirm the muscle regeneration effect in animal tissue, cardiotoxin (CTX) was used for the regeneration model. Six hours after CTX injection, gastrocnemius muscle volume was increased compared to control. Otherwise, the muscle volume of the U and A treatment groups was not changed. U and A also upregulated regeneration markers MyHC and PGC-1α in a CTX mouse model. These results indicate that seaweed extracts and alginic acid, a seaweed component, are applicable to senile sarcopenia by inhibiting muscle loss and promoting muscle regeneration.

주제어

표/그림 (4)

그림 Fig. 1. Effects of Undaria pinnatifida extracts (U) and alginate (A) on myotube differentiation in TNF-α-stimulated C2C12 cells. Cells were induced to differentiate in DM for 5 days. U (50 μg/ml) or A (10 μM) as administered alone for 24 hr and was then replaced with TNF-α (25 μg/ml) in DM. After incubation for 1 hr, myotube formation was quantified. Data are presented as the means ± SEMs of three independent experiments. #p<0.05 versus the con-trol; *p<0.05 versus TNF-α alone; **p<0.01 versus TNF-α alone. Scale bar: 100 μm.
그림 Fig. 2. Effects of Undaria pinnatifida extracts (U) and Alginate (A) on muscle atrophy biomarker (A) and ubiquitination marker (B) expression. Differentiated C2C12 were treated by U or A with TNF-α (25 μg/ml) for 1 hr and then were analysed by Western blot. The muscle atrophy marker in this study were MyHC, Murf and MaFbx. The expression were normalized to β-actin (A). The ubiquitination markers p-FoxO1 and p-smad3 were normalized by total FoxO1 and total smad3 (B). Data are presented as the means ± SEMs of three independent experiments. #p<0.05, ##p<0.01 and ###p<0.001 versus the control; *p<0.05, ***p<0.001 versus TNF-α alone.
그림 Fig. 3. Undaria pinnatifida extracts (U) and Alginate (A) regu-lated differentiation of C2C12 cells. C2C12 cells were treated with U (50 μg/ml) or A (10 μM) in the presence DM and cell lysates were prepared for western blot analysis. Levels Pax7, MyoD, MyoG MyHC were nor-malized to β-actin respectively. Representative band im-ages from individual experiments are shown in U (A) and A (B). Data are presented as the means ± SEMs of three independent experiments.
그림 Fig. 4. Undaria pinnatifida extracts (U) and Alginate (A) regulated CTX induced muscle regeneration in mouse gastrocnemius tissue. Rright gastrocnemius tissue were isolated after CTX injection (6 hr). Left gastrocnemius were injected saline for control. The muscle volume change were divided by weight and normalized to left gastrocnemius tissue (Fig. 4A). The atrophy marker and regeneration marker were measured by Westerb blot anlaysis. Each side of gastrocnemius protein lysates were blotted to Murf1, MyHC and PGC-1α. the each marker expression were normalized to GAPDH. Data are presented as the means ± SEMs of three independent experiments. ###p<0.001 versus the control; ***p<0.001 versus CTX alone.

AI 본문요약
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데이터처리

각 실험의 결과는 3번 이상 반복 실험을 하였으며 평균 ± 평균오차(mean ± S.E.M)로 표시하였으며, GraphPad prism 7.0 (GraphPad software Inc., La Jolla, CA, USA) 프로그램을 사용하여 two-way Anova, Tukey Multiple comparison으로 유의성을 분석하였다.

이론/모형

각 그룹의 모든 샘플들의 동량을 채취하여 pooling을 한 후 단백질을 정량하고 atrophy 마커인 Murf1 과 분화 마커인 MHC, PGC-1α 항체를 이용하여 분화 마커의 단백질 발현량을 웨스턴 블랏 방법으로 분석하였다.

참고문헌 (25)

Baumgartner, R. N., Koehler, K. M., Gallagher, D., Romero, L., Heymsfield, S. B., Ross, R. R. and Lindeman, R. D. 1998. Epidemiology of sarcopenia among the elderly in New Mexico. Am. J. Epidemiol. 147, 755-763.

상세보기
Bonaldo, P. and Sandri, M. 2013. Cellular and molecular mechanisms of muscle atrophy. Dis. Models Mech. 6, 25-39.

상세보기
Chen, Y. M., Tsai, Y. H., Tsai, T. Y., Chiu, Y. S., Wei, L., Chen, W. C. and Huang, C. C. 2014. Fucoidan supplementation improves exercise performance and exhibits anti-fatigue action in mice. Nutrients 7, 239-252.

상세보기
Choi, J. S., Bae, H. J., Kim, Y. C., Park, N. H., Kim, T. B., Choi, Y. J., Choi, E. Y., Park S. M. and Choi, I. S. 2008. Nutritional composition and biological activities of the methanol extracts of sea mustard (Undaria pinnatifida) in market. J. Life Sci. 18, 387-394.

원문보기 상세보기
Fong, Y., Moldawer, L. L., Marano, M., Wei, H., Barber, A., Manogue, K., Tracey, K. J., Kuo, G., Fischman, D. A. and Cerami, A. 1989. Cachectin/TNF or IL-1 alpha induces cachexia with redistribution of body proteins. Am. J. Physiol. 256, R659-665.

상세보기
Garry, G. A., Antony, M. L. and Garry, D. J. 2016. Cardiotoxin induced injury and skeletal muscle regeneration. Methods Mol. Biol. 1460, 61-71.

상세보기
Glass, D. J. 2003. Signalling pathways that mediate skeletal muscle hypertrophy and atrophy. Nat. Cell Biol. 5, 87-90.

상세보기
Guardiola, O., Andolfi, G., Tirone, M., Iavarone, F., Brunelli, S. and Minchiotti, G. 2017. Induction of acute skeletal muscle regeneration by cardiotoxin injection. J. Vis. Exp. 119, e54515, doi:10.3791/54515.

상세보기
Hardy, D., Besnard, A., Latil, M., Jouvion, G., Briand, D., Thepenier, C., Pascal, Q., Guguin, A., Gayraud-Morel, B., Cavaillon, J. M., Tajbakhsh, S., Rocheteau, P. and Chretien, F. 2016. Comparative study of injury models for studying muscle regeneration in mice. PLoS One 11, e0147198. doi: 10.1371/journal.pone.0147198.

상세보기
Kandarian, S. C. and Jackman, R. W. 2006. Intracellular signaling during skeletal muscle atrophy. Muscle Nerve 33, 155-165.

상세보기
Khan, M. N., Choi, J. S., Lee, M. C., Kim, E., Nam, T. J., Fujii, H. and Hong, Y. K. 2008. Anti-inflammatory activities of methanol extracts from various seaweed species. J. Environ. Biol. 29, 465-469.

상세보기
Kong, R., Shon, M. Y., Seo, Y. S, Kang, O. H., Zhou, T., Kim, D. Y., Choi, S. H. and Kwon, D. Y. 2016. The anti-diabetic effects and nephroprotective effect of black ginseng prosapogenin extract in streptozotocin-induced mice. Kor. J. Medicinal Crop Sci. 24, 115-120.

원문보기 상세보기
Lang, C. H., Frost, R. A., Nairn, A. C., MacLean, D. A. and Vary, T. C. 2002. TNF-alpha impairs heart and skeletal muscle protein synthesis by altering translation initiation. Am. J. Physiol. Endocrinol. Metab. 282, E336-347.

상세보기
Lepper, C., Partridge, T. A. and Fan, C. M. 2011. An absolute requirement for Pax7-positive satellite cells in acute injury-induced skeletal muscle regeneration. Development 138, 3639-3646.

상세보기
Li, J., Cai, C., Yang, C., Li, J., Sun, T. and Yu, G. 2019. Recent advances in pharmaceutical potential of brown algal polysaccharides and their derivatives. Curr. Pharm. Des. 25, 1290-1311.

상세보기
Montesano, A., Luzi, L., Senesi, P., Mazzocchi, N. and Terruzzi, I. 2013. Resveratrol promotes myogenesis and hypertrophy in murine myoblasts. J. Transl. Med. 11, 310. doi: 10.1186/1479-5876-11-310.

상세보기
Muhamad, I. I., Zulkifli, N., Selvakumaran, S. A. and Lazim, N. A. M. 2019. Bioactive algal-derived polysaccharides: multi-functionalization, therapeutic potential and biomedical applications. Curr. Pharm. Des. 25, 1147-1162.

상세보기
Puigserver, P. and Spiegelman, B. M. 2003. Peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC1 alpha): transcriptional coactivator and metabolic regulator. Endocr. Rev. 24, 78-90.

상세보기
Rolland, Y., Czerwinski, S., Abellan Van Kan, G., Morley, J. E., Cesari, M., Onder, G., Woo, J., Baumgartner, R., Pillard, F., Boirie, Y., Chumlea, W. M. C. and Vellas, B. 2008. Sarcopenia: its assessment, etiology, pathogenesis, consequences and future perspectives. J. Nutr. Health Aging 12, 433-450.

상세보기
Rosenberg, I. H. 1997. Sarcopenia: origins and clinical relevance. J. Nutr. 127, 990s-991s.

상세보기
Schiaffino, S., Dyar, K. A., Ciciliot, S., Blaauw, B. and Sandri, M. 2013. Mechanisms regulating skeletal muscle growth and atrophy. FEBS J. 280, 4294-4314.

상세보기
Taboada, C., Millan, R. and Miguez, I. 2013. Evaluation of marine algae Undaria pinnatifida and Porphyra purpurea as a food supplement: composition, nutritional value and effect of intake on intestinal, hepatic and renal enzyme activities in rats. J. Sci. Food Agric. 93, 1863-1868.

상세보기
Volpi, E. and Rasmussen, B. B. 2000. Nutrition and muscle protein metabolism in the elderly. Diabetes Nutr. Metab. 13, 99-107.
Wang, S. K., Li, Y., White, W. L. and Lu, J. 2014. Extracts from New zealand undaria pinnatifida containing fucoxanthin as potential functional biomaterials against cancer in vitro. J. Funct. Biomater. 5, 29-42.

상세보기
Wells, L., Edwards, K. A. and Bernstein, S. I. 1996. Myosin heavy chain isoforms regulate muscle function but not myofibril assembly. EMBO J. 15, 4454-4459.

상세보기

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