[논문]Pectolinarin 고함유 곤드레 미세분말의 항산화 및 항비만 활성

박성진; 김현덕; 이윤환

doi:10.9799/ksfan.2021.34.1.069

Pectolinarin 고함유 곤드레 미세분말의 항산화 및 항비만 활성
Antioxidant and Anti-Obesity Properties of Pectolinarin-rich Cirsium setidens Nakai Fine Powder 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.34 no.1, 2021년, pp.69 - 78

박성진 (한림성심대학교 관광외식조리과) , 김현덕 ((주)찬누리라이프) , 이윤환 ((주)찬누리라이프)

Abstract ▼ AI-Helper

The aim of this study was to evaluate the basic data of Cirsium setidens Nakai fine powder (FPC), which will then be used in the development of functional fooditems. We measures and evaluated the level of pectolinarin content, phenol content, flavonoids content, antioxidants and anti-obesity properties of FPC. Our results from the study showed that the pectolinarin, phenol, and flavonoids contents of FPC measured at 10.95±0.1 5mg/g, 12.92±0.18 mg gallic acid equivalent (GAE)/g and 26.47±0.77 mg rutin equivalent (RE)/g, respectively. The exhibited antioxidant activity of FPC increased significantly depending on the dosage, and additionally. FPC did not show any cytotoxicity up to the dosage level of 500 μg/mL. During adipocyte differentiation, FPC significantly inhibited ROS production and lipid accumulation, compared with the control substance. These results suggest that FPC could be considered a promising resource of natural antioxidants and could serve a variety of health-improving roles in the production of functional food ingredients.

주제어

표/그림 (9)

표 Table 1. HPLC condition for analysis of pectolinarin
표 Table 2. Pectolinarin, total phenol and total flavonoid contents of FPC (Cirsium setidens Nakai fine powder)
그림 Fig. 1. DPPH radical scavenging activity (a), FRAP value (b) and reducing power (c), ABTS radical scavenging activities (d) of FPC (Cirsium setidens Nakai fine powder). Each bar represents the mean±S.D. of quadraplicate determinations, n=3. Statistical analysis was performed using the one-way ANOVA (p<0.05).
표 Table 3. Correlation analysis (R) among the antioxidant activity and antioxidant compounds of Cirsium setidens Nakai fine powder
그림 Fig. 2. Effects of FPC (Cirsium setidens Nakai fine powder) on cell viability in 3T3-L1 adipocytes. Results are presented as the mean±S.D. of 3 independent in triplicate. Means with different letters on the same kind of bars are significantly different at p<0.05 by Duncan's multiple range test.
그림 Fig. 3. Effects of FPC (Cirsium setidens Nakai fine powder) on lipid accumulation in 3T3-L1 adipocytes. Results are presented as the mean±S.D. of 3 independent in triplicate. Means with different letters on the same kind of bars are signi-ficantly different at p<0.05 by Duncan's multiple range test.
그림 Fig. 4. Effects of FPC (Cirsium setidens Nakai fine powder) on ROS production in 3T3-L1 adipocytes. Results are presented as the mean±S.D. of 3 independent in triplicate. Means with different letters on the same kind of bars are significantly different at p<0.05 by Duncan's multiple range test.
그림 Fig. 5. Effects of FPC (Cirsium setidens Nakai fine powder) on the expression levels of adipocyte-specific transcription factors in 3T3-L1 adipocytes. All values are presented as the mean±S.D. Bars with different letters indicate statistically significant differences among groups at p<0.05 by one-way ANOVA.
그림 Fig. 6. Effects of FPC (Cirsium setidens Nakai fine powder) on the expression levels of p-AMPK and p-HSL proteins in 3T3-L1 adipocytes. All values are presented as the mean±S.D. Bars with different letters indicate statistically significant differences among groups at p<0.05 by one-way ANOVA.

참고문헌 (37)

Ahn MJ, Hur SJ, Kim EH, Lee SH, Shin JS, Kim MK, Uchizono JA, Whang WK, Kim DS. 2014. Scopoletin from Cirsium setidens increases melanin synthesis via CREB phosphorylation in B16F10 cells. Korean J Physiol Pharmacol 18:307-311

원문보기 상세보기
Benzie IFF, Stranin JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem 239:70-76

상세보기
Cho BY, Park MR, Lee JH, Ra MJ, Han KC, Kang IJ, Lee OH. 2017. Standardized Cirsium setidens Nakai ethanolic extract suppresses adipogenesis and regulates lipid metabolisms in 3T3-L1 adipocytes and C57BL/6J mice fed high-fat diets. J Med Food 20:763-776

상세보기
Cho ML, Yoon SJ, Kim YB. 2013. The nutritional composition and antioxidant activity from Undariopsis peterseniana. Ocean Polar Res 35:273-280

원문보기 상세보기
Cho ML, Lee HS, Kang IJ, Won MH, You SG. 2011. Antioxidant properties of extract and fractions from Enteromorpha prolifera, a type of green seaweed. Food Chem 127:999-1006

상세보기
Choi HY, Kim GH. 2014. Inhibitory effects of Allium sacculiferum Max. methanol extracts on ROS production and lipid accumulation during differentiation of 3T3-L1 cells. J Korean Soc Food Sci Nutr 43:822-828

원문보기 상세보기
Choi SI, Lee JS, Lee S, Lee HJ, Yeo J, Cho BY, Lee JH, Kim JM, Jung TD, Choi SH, Kim JY, Kang SN, Lee OK. 2017. Effects of extracts of five species of Korean native forest plants on lipid accumulation and reactive oxygen species production during differentiation of 3T3-L1 preadipocytes. J Korean Soc Food Sci Nutr 46:523-528

원문보기 상세보기
Chung MJ, Lee S, Park YI, Lee J, Kwon KH. 2016. Neuroprotective effects of phytosterols and flavonoids from Cirsium setidens and Aster scaber in human brain neuroblastoma SK-N-SH cells. Life Sci 148:173-182

상세보기
Do GP, Lee HJ, Do JR, Kim HK. 2012. Antiobesity effect of the Cucubita moschata Duch extracts in 3T3-L1 adipocytes. Korean J Food Preserv 19:138-143

원문보기 상세보기
Han X, Choi SI, Kim MJ, Lee OH. 2019. Evaluation of antioxidant and cognition improvement effects of 10 wild vegetables cultivated in Gangwon province. Korean J Food Nutr 32:662-668
Hsu CL, Yen GC. 2007. Effects of flavonoids and phenolic acids on the inhibition of adipogenesis in 3T3-L1 adipocytes. J Agric Food Chem 55:8404-8410

상세보기
Hwang EG, Kim SJ, Kim BK. 2014. Effects of the characteristics of wild vegetables on customer satisfaction, trust and repurchase intention. Korean J Culin Res 20:59-74
Jung EY, Lee SK 2007. Anti-oxidant activities and regeneration effect in HaCaT cell line by Jeju Island aboriginal Eclonia cava. J Korean Soc Cosmetol 13:1071-1077
Kim DJ, Jung JH, Kim SG, Lee HK, Lee SK, Hong HD, Lee BY, Lee OH. 2011a. Antioxidants and anti-obesity activities of hot water and ethanolic extracts from Cheonnyuncho (Opuntia humifusa). Korean J Food Preserv 18:366-373

원문보기 상세보기
Kim IS, Yang MR, Lee OH, Kang SN. 2011b. Antioxidant activities of hot water extracts from various spices. Int J Mol Sci 12:4120-4131

상세보기
Kim JH, Park JH, Park SD, Choi SY, Seong JH, Hoon KD. 2002a. Preparation and antioxidant activity of health drink with extract powders from safflower (Carthamus tinctorius L.) seed. Korean J Food Sci 34:617-624
Kim JH, Park JH, Park SD, Kim JK, Kang WW, Moon KD. 2002b. Effect of addition of various mesh sifted powders from safflower seed on quality characteristic of Yangeng. Korean J Food Preserv 9:309-313
Lee JH, Jung HK, Han YS, Yoon YM, Yun CW, Sun HY, Cho HW, Lee SH. 2016. Antioxidant effects of Cirsium setidens extract on oxidative stress in human mesenchymal stem cells. Mol Med Rep 14:3777-3784

상세보기
Lee JH, Moon SY, Cho BY, Choi SI, Jung TD, Choi SH, Kim JD, Lee OH. 2017. Stability of ethanolic extract from fermented Cirsium setidens Nakai by bioconversion during different storing conditions. Korean J Food Nutr 30:388-394

원문보기 상세보기
Lee MY, Yoo MS, Whang YJ, Jin YJ, Hong MH, Pyo YH. 2012. Vitamin C, total polyphenol, flavonoid contents and antioxidant capacity of several fruit peels. Korean J Food Sci Technol 44:540-544

원문보기 상세보기
Lee SH, Jin YS, Heo SI, Shim TH, Sa JH, Choi DS, Wang MH. 2006. Composition analysis and antioxidative activity from different organs of Cirsium setidens Nakai. Korean J Food Sci Technol 38:571-576
Lee WB, Kwon HC, Cho OR, Lee KC, Choi SU, Baek NI, Lee KR. 2002. Phytochemical constituents of Cirsium setidens Nakai and their cytotoxicity against human cancer cell lines. Arch Pharmacal Res 25:628-635

원문보기 상세보기
Lee YJ, Lee JH, Kim YH, Kim JH, Yu SY, Kim DB, Lee JS, Cho ML, Cho JH, Kim BK, Lee BY, Lee OH. 2015. Assessment of the pectolinarin content and the radical scavenging-linked antiobesity activity of Cirsium setidens Nakai extracts. Food Sci Biotechnol 24:2235-2243

상세보기
Li H, Choi YM, Lee JS, Park JS, Yeon KS, Han CS. 2007. Drying and antioxidant characteristics of the shiitake (Lentinus edodes) mushroom in a conveyer-type far-infrared dryer. J Korean Soc Food Sci Nutr 36:250-254

원문보기 상세보기
Miquel J, Quintaniha AT, Weber H. 1989. Handbook of Free Radicals and Antioxidants in Biomedicine. Vol. 1. pp.223-244. CRC Press
Noh H, Lee H, Kim E, Mu L, Rhee YK, Cho CW, Chung J. 2013. Inhibitory effect of a Cirsium setidens extracts on hepatic fat accumulation in mice fed a high-fat diet via the induction of fatty acid β-oxidation. Biosci Biotechnol Biochem 77:1424-1429

상세보기
Oh JW, Lee JH, Cho ML, Shin GH, Kim JM, Choi SI, Jung TD, Kim YH, Lee SJ, Lee BJ, Park SJ, Lee OH. 2015. Development and validation of analytical method for pectolinarin and pectolinarigenin in fermented Cirsium setidens Nakai by bioconversion. J Korean Soc Food Sci Nutr 44:1504-1509

원문보기 상세보기
Park DJ, Ku KH, Kim SH. 1996. Characteristics and application of defatted soybean meal fractions obtained by microparticulation/air-classification. Korean J Food Sci Technol 28:497-505
Park SJ, Kim HD, Lee YH. 2020. Validation of analytical method of pectolinarin for standardized Cirsium setidens fine powder by air-classification. J Agric Life Environ Sci 32:407-416
Prior RL, Cao G, Martin A, Sofic E, McEwen J, O'Brien C, Lischner N, Ehlenfeldt M, Kalt W, Krewer G, Mainland CM. 1998. Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity, and variety of Vaccinium species. J Agric Food Chem 46:2686-2693

상세보기
Que F, Mao L, Zhu C, Xie G. 2006. Antioxidant properties of Chineses yellow wine, its concentrate and volatiles. LWTFood Sci Technol 39:111-117
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231-1237

상세보기
Student AK, Hsu RY, Lane MD. 1980. Induction of fatty acid synthetase synthesis in differentiating 3T3-L1 preadipocytes. J Biol Chem 225:4745-4750

상세보기
Thao NTP, Cuong TD, Hung TM, Lee JH, Na M, Son JK, Jung HJ, Fang Z, Woo MH, Choi JS, Min BS. 2011. Simultaneous determination of bioactive flavonoids in some selected Korean thistles by highperformance liquid chromatography. Arch Pharmacal Res 34:455-461

원문보기 상세보기
Yang HJ, Park MJ, Lee HS. 2011. Antioxidative activities and components of Gardenia jasminoides. Korean J Food Sci Technol 43:51-57

원문보기 상세보기
Yoon BR, Cho BJ, Lee HK, Kim DJ, Rhee SK, Hong HD, Kim KT, Cho CW, Choi HS, Lee BY, Lee OH. 2012. Antioxidant and anti-adipogenic effects of ethanolic extracts from Tartary and common buckwheats. Korean J Food Preserv 19:123-130

원문보기 상세보기
You SG, Kim SW, Jung KH, Moon SK, Yu KW, Choi WS. 2010. Effect of Astragali Radix and Opuntia humifusa on quality of red ginseng drink. Food Eng Prog 14:299-306

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