[논문]Physiological Characteristics and Anti-Diabetic Effect of Pediococcus pentosaceus KI62

Kim, Seulki; Hong, Sang-pil; Lim, Sang-Dong

doi:10.5851/kosfa.2020.e99

Physiological Characteristics and Anti-Diabetic Effect of Pediococcus pentosaceus KI62 원문보기

Food science of animal resources, v.41 no.2, 2021년, pp.274 - 287

Kim, Seulki (Korea Food Research Institute) , Hong, Sang-pil (Korea Food Research Institute) , Lim, Sang-Dong (Korea Food Research Institute)

Abstract ▼ AI-Helper

The purpose of this study is to examine the physiological characteristics and anti-diabetic effects of Pediococcus pentosaceus KI62. The α-amylase and α-glucosidase inhibitory activity of P. pentosaceus KI62 was 94.86±3.30% and 98.59±0.52%, respectively. In MRS broth containing 3% maltodextrin inoculated by P. pentosaceus KI62, the amounts of short chain fatty acids (SCFA) were propionic acid 18.05±1.85 mg/kg, acetic acid 1.12±0.07 g/100 mL, and butyric acid 2.19±0.061 g/kg, and those of medium chain fatty acids (MCFA) were C8 0.262±0.031 mg/kg, C10 0.279±0.021 mg/kg, and C12 0.203±0.009 mg/kg. Compared to sixteen antibiotics, P. pentosaceus KI62 had the highest sensitivity to penicillin-G and rifampicin, as well as the highest resistance to vancomycin and ampicillin. The strain also showed higher leucine arylamidase and valine arylamidase activities than other enzyme activities, but it did not produce β-glucuronidase which is carcinogenic enzymes. The survival rate of P. pentosaceus KI62 in 0.3% bile was 91.67%. Moreover, the strain showed a 98.63% survival rate in pH 2.0. P. pentosaceus KI62 exhibits resistance to Escherichia coli, Salmonella Typhimurium, Listeria monocytogenes, and Staphylococcus aureus at rates of 29.41%, 38.10%, 51.72%, and 50.47%, respectively. P. pentosaceus (23.31%) showed a similar adhesion ability to L. rhamnosus GG, the positive control (24.49%). These results show that P. pentosaceus KI62 has possibility as a probiotic with anti-diabetic effects.

주제어

표/그림 (11)

표 Table 1. Specification and operating condition of GC for propionic acid analysis
표 Table 2. Specification and operating condition of GC for butyric acid analysis
표 Table 3. Selected lactic acid bacteria having anti-diabetes (%)
그림 Fig. 1. Production of short chain fatty acid of Pediococcus pentosaceus KI62 in MRS broth and MRS broth with 3% maltodextrin. * p<0.05 between with maltodextrin and without maltodextrin (t-test).
그림 Fig. 2. Production of medium chain fatty acid of Pediococcus pentosaceus KI62 in MRS broth and MRS broth with 3% maltodextrin. ^NS Means that the values are not significantly different between with maltodextrin and without maltodextrin (t-test).
표 Table 4. Antibiotics susceptibility of Pediococcus pentosaceus KI62
표 Table 5. Enzyme patterns of Pediococcus pentosaceus KI62
그림 Fig. 3. Growth of Pediococcus pentosaceus KI62 in MRS broth containing 0.05% L-cysteine with/without 0.3% oxgall. Values are mean±SD of the three replicates; * p<0.05, ** p<0.01, and *** p<0.001 between with ox gall and without oxgall (t-test).
그림 Fig. 4. Survival of Pediococcus pentosaceus KI62 after 3 h in HCl solution. Values are mean±SD of the three replicates; * p<0.05 and ** p<0.01 compared with initial time (t-test).
그림 Fig. 5. Adhesion ability of Pediococcus pentosaceus KI62 to HT-29 cell. Values are mean±SD of the three replicates. ^NS Means that the values are not significantly different compared with Lactobacillus rhamnosus GG (t-test, p<0.05).
표 Table 6. Inhibition of pathogens by Pediococcus pentosaceus KI62 in MRS broth

AI 본문요약
AI-Helper

문제 정의

This study was conducted to investigate the anti-diabetic effects of P. pentosaceus KI62 selected from among LAB isolated from kimchi, and to study its physiological characteristics to confirm the potential of health functional food or fermented milk as a starter.

이론/모형

Each experiment was performed in triplicate, and the results were displayed as the mean±SD. Statistical analysis was performed using a XLSTAT (Addinsoft, Paris, France). All analysis was conducted on p<0.

성능/효과

47%, respectively. It exhibited 23.31% adherence to the epithelial surface. These results demonstrate that P.
pentosaceus KI62 has the best bile and acid tolerance ability. It showed resistance to E. coli, S.
P
003 g/kg. The contents of MCFAs in MRS broth containing 3% maltodextrin inoculated by P. pentosaceus KI62 were C8 0.
52%, respectively. The contents of SCFA in MRS broth containing 3% maltodextrin inoculated by P. pentosaceus KI62 were propionic acid 8.
31% adherence to the epithelial surface. These results demonstrate that P. pentosaceus KI62 has potential as a probiotic with anti-diabetic effects.
monocytogenes, and S. aureus at rates of 29.41%, 38.10%, 51.72%, and 50.47%, respectively (Table 6). The pH value of pathogens after incubation for 6 h was around 5.
Typhimurium, L. monocytogenes, and S. aureus at rates of 29.
This study was conducted to investigate the antidiabetic effect and physiological characteristics of P. pentosaceus KI62 to determine whether P. pentosaceus KI62 isolated from kimchi can be applied as a functional food or fermented milk.
These results demonstrate that P. pentosaceus KI62 has potential as a probiotic with anti-diabetic effects.
In other words, P. pentosaceus KI62 has the best acid and bile tolerance ability because a relatively high percentage of the strain survived in MRS broth adding 0.3% bile salt as well as under a highly acidic condition.
P. pentosaceus KI62 has the best bile and acid tolerance ability. It showed resistance to E.
Consequently, the survival rate of P. pentosaceus KI62 in MRS broth containing 0.3% bile was 91.67% (Fig. 3).
This study was conducted to investigate the anti-diabetic effects of P. pentosaceus KI62 selected from among LAB isolated from kimchi, and to study its physiological characteristics to confirm the potential of health functional food or fermented milk as a starter. On the basis of the nucleotide sequence of 16S rDNA gene, it was named P.
In a comparison of sixteen different antibiotics, P. pentosaceus KI62 showed higher sensitivity to penicillin-G, rifampicin, and clindamycin, as well as the highest resistance to vancomycin and ampicillin.
pentosaceus KI62 strain was observed to exhibit α-amylase and α-glucosidase inhibitory activity of 94.86±3.30% and 98.59±0.52%, respectively
pentosaceus KI62 were C8 0.262±0.031 mg/kg, C10 0.279±0.021 mg/kg, and C12 0.203±0.009 mg/kg
pentosaceus KI62 were propionic acid 8.78±1.12 mg/kg, acetic acid 1.34±0.07 g/100 mL, and butyric acid 0.876±0.003 g/kg

참고문헌 (43)

Ahmadova A Todorov SD Choiset Y Rabesona H Zadi TM Kuliyev A Franco BDG Chobert JM Haertlé T 2013 Evaluation of antimicrobial activity, probiotic properties, and safety of wild strain Enterococcus faecium AQ71 isolated from Azerbaijani Motal cheese Food Control 30 631 641 10.1016/j.foodcont.2012.08.009

상세보기
Ali H Houghton PJ Soumyanath A 2006 α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus J Ethnopharmacol 107 449 455 10.1016/j.jep.2006.04.004 16678367

상세보기
Banwo K Sanni A Tan H 2013 Functional properties of Pediococcus species isolated from traditional fermented cereal gruel and milk in Nigeria Food Biotechnol 27 14 38 10.1080/08905436.2012.755626

상세보기
Bao Y Zhang Y Zhang Y Liu Y Wang S Dong X Wang Y Zhang H 2010 Screening of potential probiotic properties of Lactobacillus fermentum isolated from traditional dairy products Food Control 21 695 701 10.1016/j.foodcont.2009.10.010

상세보기
Bernet MF Brassart D Neeser JR Servin AL 1993 Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interactions Appl Environ Microbiol 59 4121 4128 10.1128/AEM.59.12.4121-4128.1993 8285709

상세보기
Bird AR Brown IL Topping DL 2000 Strarches, resistant starches, the gut microflora and human health Curr Issues Intest Microbiol 1 25 37 11709851
Blum S Reniero R Schiffrin EJ Crittenden R Mattila-Sandholm T Ouwehand AC Salminen S von Wright A Saarela M Saxelin M Collins K Morelli L 1999 Adhesion studies for probiotics: Need for validation and refinement Trends Food Sci Technol 10 405 410 10.1016/S0924-2244(00)00028-5

상세보기
Chesson A Franklin A Aumaître A Sköld O Leclercq R von Wright A Guillot JF 2002 Opinion of the scientific committee on animal nutrition on the criteria for assessing the safety of micro-organisms resistant to antibiotics of human clinical and veterinary importance European Commission, Health and Consumer Protection Directorate General Brussels, Belgium
Clark PA Cotton LN Martin JH 1993 Selection of bifidobacteria for use as dietary adjuncts in cultured dairy foods: II-Tolerance to simulated pH of human stomachs Cult Dairy Prod J 28 11 14
Cummings JH 1981 Short chain fatty acids in human colon Gut 22 763 779 10.1136/gut.22.9.763 7028579

상세보기
Dabek M McCrae SI Stevens VJ Duncan SH Louis P 2008 Distribution of β-glucosidase and β-glucuronidase activity and of β-glucuronidase gene gus in human colonic bacteria FEMS Microbiol Ecol 66 487 495 10.1111/j.1574-6941.2008.00520.x 18537837

상세보기
Danielsen M Simpson PJ O’Connor EB Ross RP Stanton C 2007 Susceptibility of Pediococcus spp. to antimicrobial agents J Appl Microbiol 102 384 389 10.1111/j.1365-2672.2006.03097.x 17241343

상세보기
De Verse M Stegelmann A Richter B Fenselau S Laue C Schrezenmeir J 2001 Probiotics-compensation for lactase insufficiency Am J Clin Nutr 73 421s 429s 10.1093/ajcn/73.2.421s 11157352

상세보기
European Food Safety Authority [EFSA] 2008 Technical guidance prepared by the panel on additives and products or substances in animal feed (FEEDAP) on the update of the criteria used in the assessment of bacterial resistance to antibiotics of human and veterinary importance EFSA J 732 1 15
Gilliand SE Speck ML 1977 Deconjugation of bile acids by intestinal lactobacilli Appl Environ Micobiol 33 15 18 10.1128/AEM.33.1.15-18.1977 13710

상세보기
Gilliland SE Walker DK 1990 Factors to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjunct to produce a hypocholesterolemic effect in humans J Dairy Sci 73 905 911 10.3168/jds.S0022-0302(90)78747-4 2111831

상세보기
Gopal PK Prasad J Smart J Gill HS 2001 In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Esherichia coli Int J Food Microbiol 67 207 216 10.1016/S0168-1605(01)00440-8 11518430

상세보기
Henriksson R Bergström P Franzén L Lewin F Wagenius G 1999 Aspects of reducing gastrointestinal adverse effects associated with radiotherapy Acta Oncologica 38 159 164 10.1080/028418699431564 10227436

상세보기
Hummel AS Hertel C Holzapfel WH Franz CMA 2007 Antibiotic resistances of starter and probiotic strains of lactic acid bacteria Appl Environ Microbiol 73 730 739 10.1128/AEM.02105-06 17122388

상세보기
Jang YS Jeong JM 2010 Antioxidative effect and digestive enzyme inhibition of grape seed extract (GSE) J Korean Soc Food Sci Nutr 39 783 788 10.3746/jkfn.2010.39.6.783

원문보기 상세보기
Kesarcodi-Watson A Miner P Nicolas JL Robert R 2012 Protective effect of four potential probiotics against pathogen-challenge of the larvae of three bivalves: Pacific oyster ( Crassostrea gigas ), flat oyster ( Ostrea edulis ), and scallop ( Pecten maximus ) Aquaculture 344 29 34 10.1016/j.aquaculture.2012.02.029

상세보기
Kim SJ Cho SY Kim SH Song OJ Shin IS Cha DS Park HJ 2008 Effect of microencapsulation on viability and other characteristics in Lactobacillus acidophilus ATCC 43121 LWT-Food Sci Technol 41 493 500 10.1016/j.lwt.2007.03.025

상세보기
Klare I Konstabel C Werner G Huys G Vankerckhoven V Kahlmeter G Hildebrandt B Muller-Bertling S Witte W Goossens H 2007 Antimicrobial susceptibilities of Lactobacillus , Pediococcus and Lactococcus human isolates and cultures intended for probiotic or nutritional use J Antimicrob Chemother 59 900 912 10.1093/jac/dkm035 17369278

상세보기
Lee YK Puong KY Ouwehand AC Salminen S 2003 Displacement of bacterial pathogens from mucus and Caco-2 cell surface Lactobacilli J Med Microbiol 52 925 930 10.1099/jmm.0.05009-0 12972590

상세보기
Lee YK Salminen S 1995 The coming age of probiotics Trends Food Sci Technol 6 241 245 10.1016/S0924-2244(00)89085-8

상세보기
Lim SD Kim KS Do JR 2011 Physiological characteristics and production of vitamin K 2 by Lactobacillus fermentum LC272 isolated from raw milk Korean J Food Sci Anim Resour 31 513 520 10.5851/kosfa.2011.31.4.513

원문보기 상세보기
Louis P Scott KP Duncan SH Flint HJ 2007 Understanding the effects of diet on bacterial metabolism in the large intestine J Appl Microbiol 102 1197 1208 10.1111/j.1365-2672.2007.03322.x 17448155

상세보기
Maragkoudakis PA Zoumpopoulou G Miaris C Kalantzopoulos G Pot B Tsakalidou E 2006 Probiotic potential of Lactobacillus strains isolated from dairy products Int Dairy J 16 189 199 10.1016/j.idairyj.2005.02.009

상세보기
Martín R Olivares M Marín ML Fernández L Xaus J Rodríguez JM 2005 Probiotic potential of 3 lactobacilli strains isolated from breast milk J Hum Lact 21 8 17 10.1177/0890334404272393 15681631

상세보기
Phillips I 1991 A guide to sensitivity testing. Report of the working party on Antibiotic sensitivity testing of the British Society for Antimicrobial Chemotherapy J Antimicrob Chemother 27 1 50
Rhee SJ Lee JE Lee CH 2011 Importance of lactic acid bacteria in Asian fermented foods Microb Cell Fact 10 1 5 10.1186/1475-2859-10-S1-S5 21211066

상세보기
Rittiphairoj T Pongpirul K Mueller NT Li T 2019 Probiotics for glycemic control in patients with type 2 diabetes mellitus: Protocol for a systematic review Syst Rev 8 227 10.1186/s13643-019-1145-y 31481125

상세보기
Schwiertz A Taras D Schäfer K Beijer S Bos NA Donus C Hardt PD 2010 Microbiota and SCFA in lean and overweight healthy subjects Obesity 18 190 195 10.1038/oby.2009.167 19498350

상세보기
Si MM Lou JS Zhou CX Shen JN Wu HH Yang Bo He QJ Wu HS 2010 Insulin releasing and alpha-glucosidase inhibitory activity of ethyl acetate fraction of Acorus calamus in vitro and in vivo J Ethnopharmacol 128 154 159 10.1016/j.jep.2009.12.044 20051258

상세보기
Succi M Tremonte P Reale A Sorrentino E Grazia L Pacifico S Coppola R 2005 Bile salt and acid tolerance of Lactobacillus rhamnosus strains isolated from Parmigiano Reggiano cheese FEMS Microbiol Lett 244 129 137 10.1016/j.femsle.2005.01.037 15727832

상세보기
Topping DL Clifton PM 2001 Short-chain fatty acids and human colonic function: Roles of resistant starch and nonstarch polysaccharides Physiol Rev 81 1031 1064 10.1152/physrev.2001.81.3.1031 11427691

상세보기
Turner N Hariharan K TidAng J Frangioudakis G Beale SM Wright LE Zeng XY Leslie SJ Li JY Kraegen EW Cooney GJ Ye JM 2009 Enhancement of muscle mitochondrial oxidative capacity and alterations in insulin action are lipid species dependent: Potent tissue-specific effects of medium-chain fatty acids Diabetes 58 2547 2554 10.2337/db09-0784 19720794

상세보기
Tzanetakis N Litopoulou-Tzanetaki E 1989 Biochemical activities of Pediococcus pentosaceus isolates of dairy origin J Dairy Sci 72 859 863 10.3168/jds.S0022-0302(89)79178-5

상세보기
Vidhyasagar V Jeevaratnam K 2013 Evaluation of Pediococcus pentosaceus strains isolated from idly batter for probiotic properties in vitro J Func Foods 5 235 243 10.1016/j.jff.2012.10.012

상세보기
Wein S Wolffrarm S Schrezenmeir J Gašperiková D Klimeš I Šebokövá E 2009 Medium-chain fatty acids ameliorate insulin resistance caused by high-fat diets in rats Diabetes Metab Res Rev 25 185 194 10.1002/dmrr.925 19219861

상세보기
Xiao Z Storms R Tsang A 2006 A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities Anal Biochem 351 146 148 10.1016/j.ab.2006.01.036 16500607

상세보기
Yan F Li N Shi J Li H Yue Y Jiao W Wang N Song Y Huo G Li B 2019 Lactobacillus acidophilus alleviates type 2 diabetes by regulating hepatic glucose, lipid metabolism and gut microbiota in mice Food Funct 10 5804 5815 10.1039/C9FO01062A 31461095

상세보기
Zhao L Zhang F Ding X Wu G Lam YY Wang X Fu H Xue X Lu C Ma J Yu L Xu C Ren Z Xu Y Xu S Shen H Zhu X Shi Y Shen Q Dong W Liu R Ling Y Zeng Y Wang X Zhang Q Wang J Wang L Wu Y Zeng B Wei H Zhang M Peng Y Zhang C 2018 Gut bacteria selectively promoted by dietary fibers alleviate type 2 diabetes Science 359 1151 1156 10.1126/science.aao5774 29590046

상세보기

LOADING...

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증