[논문]Prevention of Cholesterol Gallstone Formation by Lactobacillus acidophilus ATCC 43121 and Lactobacillus fermentum MF27 in Lithogenic Diet-Induced Mice

Oh, Ju Kyoung; Kim, You Ra; Lee, Boin; Choi, Young Min; Kim, Sae Hun

doi:10.5851/kosfa.2020.e93

Prevention of Cholesterol Gallstone Formation by Lactobacillus acidophilus ATCC 43121 and Lactobacillus fermentum MF27 in Lithogenic Diet-Induced Mice 원문보기

Food science of animal resources, v.41 no.2, 2021년, pp.343 - 352

Oh, Ju Kyoung (Department of Food Bioscience and Technology, Korea University) , Kim, You Ra (Department of Food Bioscience and Technology, Korea University) , Lee, Boin (Department of Animal Sciences and Biotechnology, Kyungpook National University) , Choi, Young Min (Department of Animal Sciences and Biotechnology, Kyungpook National University) , Kim, Sae Hun (Department of Food Bioscience and Technology, Korea University)

Abstract ▼ AI-Helper

The objective of this study was to evaluate the effects of Lactobacillus acidophilus ATCC 43121 and L. fermentum MF27 on biochemical indices in the serum, cholesterol metabolism in the liver and mucin expression in the gallbladder in lithogenic diet (LD)-induced C57BL/6J mice to determine the preventive effects of lactobacilli on gallstone formation. By the end of 4 wk of the experimental period, mice fed on a LD with high-fat and high-cholesterol exhibited higher levels of total and low-density lipoprotein cholesterol in the serum compared to mice fed on control diet or LD with L. acidophilus ATCC 43121 (LD+P1; p<0.05). Cholesterol-lowering effects observed in the LD+P1 and LD with L. fermentum MF27 (LD+P2) groups were associated with reduced expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase in the liver compared to the LD group (p<0.05). Furthermore, expression of the gel-forming mucin, including MUC5AB and MUC5B, was suppressed in the LD+P1 and LD+P2 groups compared to the LD group (p<0.05). Therefore, steady intake of both L. acidophilus ATCC 43121 and L. fermentum MF27 may have the ability to prevent the formation of cholesterol gallstones in LD-induced C57BL/6J mice.

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표/그림 (4)

그림 Fig. 1. Comparison of body weight between the experimental groups of C57BL/6J mice. Error bars represent standard errors. Level of significance: * p<0.05, *** p<0.001. LD, lithogenic diet; UDCA, ursodeoxycholic acid; P1, Lactobacillus acidophillus ATCC 43121; P2, Lactobacillus fermentum MF27.
표 Table 1. Primer sequences of real-time PCR for gene amplification
표 Table 2. Comparison of serum biochemical indices between the experimental groups of C57BL/6J mice
그림 Fig. 2. Comparison of expression levels of genes involved in cholesterol metabolism in liver (A) and gallstone formation in gallbladder (B) by quantitative real-time PCR between the experimental groups of C57BL/6J mice. Error bars indicate standard errors. ^a-e Different letters were considered statistically different (p<0.05). HMG CoA R, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; CYP7Al, cholesterol 7α-hydroxylase; MUC, mucin; LD, lithogenic diet; UDCA, ursodeoxycholic acid; P1, Lactobacillus acidophillus ATCC 43121; P2, Lactobacillus fermentum MF27.

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데이터처리

To compare the body weight, serum biochemical indices, and expressions of various genes involved in cholesterol synthesis and gallstone formation among the five groups, association analysis by the general linear model was carried out using SAS software (SAS Institute Cary, NC, USA). Significant differences among the five treatments were determined using the probability difference (PDIFF), based on a significant value of 5%. All means were presented as least square means with standard errors.
To compare the body weight, serum biochemical indices, and expressions of various genes involved in cholesterol synthesis and gallstone formation among the five groups, association analysis by the general linear model was carried out using SAS software (SAS Institute Cary, NC, USA). Significant differences among the five treatments were determined using the probability difference (PDIFF), based on a significant value of 5%.

성능/효과

acidophilus ATCC 43121, were mediated by a decreased expression of HMG CoA R in the liver. Additionally, these properties may contribute to decreased expression of gel-forming mucins, including MUC5AC and MUC5B, in the gallbladder. However, no differences were detected in the expressions of mucins between two strains.
Additionally, these properties may contribute to decreased expression of gel-forming mucins, including MUC5AC and MUC5B, in the gallbladder. However, no differences were detected in the expressions of mucins between two strains. Thus, both lactobacilli have the preventive effect against the formation of cholesterol gallstones in the gallbladder in LD- induced C57BL/6J mice.
Thus, both lactobacilli have the preventive effect against the formation of cholesterol gallstones in the gallbladder in LD- induced C57BL/6J mice. Therefore, a steady intake of these lactobacilli can be used clinically to prevent the formation of cholesterol gallstones.
fermentum MF27 positively affect the biochemical indices of the serum without impairing the growth rate in LD-induced C57BL/6J mice after 4 wk of treatment. These hypocholesterolemic effects in the serum, more evidently seen from L. acidophilus ATCC 43121, were mediated by a decreased expression of HMG CoA R in the liver.
However, no differences were detected in the expressions of mucins between two strains. Thus, both lactobacilli have the preventive effect against the formation of cholesterol gallstones in the gallbladder in LD- induced C57BL/6J mice. Therefore, a steady intake of these lactobacilli can be used clinically to prevent the formation of cholesterol gallstones.
These hypocholesterolemic effects in the serum, more evidently seen from L. acidophilus ATCC 43121, were mediated by a decreased expression of HMG CoA R in the liver. Additionally, these properties may contribute to decreased expression of gel-forming mucins, including MUC5AC and MUC5B, in the gallbladder.
acidophilus ATCC 43121 and L. fermentum MF27 positively affect the biochemical indices of the serum without impairing the growth rate in LD-induced C57BL/6J mice after 4 wk of treatment. These hypocholesterolemic effects in the serum, more evidently seen from L.

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