그림 Fig. 1. DPPH radical scavenging activity of A. lancifolium leaf extracts Data are expressed as mean±standard deviation of triplicate experiments. Abbreviations: RSA, radical scavenging activity; AW, A. lancifolium water extract; AM, A. lancifolium methanol extract; AA, L-ascorbic acid (15 ㎍/mL). 그림 Fig. 2. ABTS radical scavenging activity of A. lancifolium leaf extracts Data are expressed as mean±standard deviation of triplicate experiments. Abbreviations: RSA, radical scavenging activity; AW, A. lancifolium water extract; AM, A. lancifolium methanol extract; AA, L-ascorbic acid (50 ㎍/mL). 표 Table 1. DPPH, ABTS Radicals scavenging assay and total polypphenol contents of A. lancifolium extracts 그림 Fig. 3. Effect of A. lancifolium leaf extracts on cell viability of RAW264.7 macrophages The cells were treated with various concentrations of leaf extracts for 24 h. Cell viability was assessed with MTT assay. Data are expressed as mean±standard deviation of triplicate experiments. Abbreviations: CTL, Control; LPS, lipopolysaccharide; AW, A. lancifolium water extract; AM, A. lancifolium methanol extract. 그림 Fig. 4. Effect of A. lancifolium leaf extracts on the NO production and iNOS protein levels in LPS-stimulated RAW264.7 cells RAW264.7 cells were pretreated with the indicated concentrations of leaf extracts for 1 h prior to LPS (100 ng/mL) for 24 h. The NO concentrations in medium were determined by Griess reagent assay (a). Whole cell lysates were then subjected to Western blot analysis(b). Data are expressed as mean±standard deviation of triplicate experiments. Abbreviations: iNOS, inducible nitric oxide synthase; LPS, lipopolysaccharide; AW, A. lancifolium water extract; AM, A. lancifolium methanol extract. 그림 Fig. 5. Inhibitory effect of A. lancifolium leaf extracts on the secretion levels of IL-6(a) and TNF-α(b) in LPS-stimulated RAW264.7 cells RAW264.7 cells were pretreated with the indicated concentrations of leaf extracts for 1 h prior to LPS (100 ng/mL) for 24 h. Cytokine secretion was determined in culture supernatant by ELISA. Data are expressed as mean±standard deviation of triplicate experiments. Abbreviations: CTL, Control; AW, A. lancifolium water extract; AM, A. lancifolium methanol extract. 그림 Fig. 6. Effect of A. lancifolium leaf extracts on the astringent activity Data are expressed as mean±standard deviation of triplicate experiments. Abbreviations: AA, L-ascorbic acid(10 mg/L); GA, Gallic acid(10 mg/mL); AW, A. lancifolium water extract; AM, A. lancifolium methanol extract.
그림 Fig. 1. DPPH radical scavenging activity of A. lancifolium leaf extracts Data are expressed as mean±standard deviation of triplicate experiments. Abbreviations: RSA, radical scavenging activity; AW, A. lancifolium water extract; AM, A. lancifolium methanol extract; AA, L-ascorbic acid (15 ㎍/mL). 그림 Fig. 2. ABTS radical scavenging activity of A. lancifolium leaf extracts Data are expressed as mean±standard deviation of triplicate experiments. Abbreviations: RSA, radical scavenging activity; AW, A. lancifolium water extract; AM, A. lancifolium methanol extract; AA, L-ascorbic acid (50 ㎍/mL). 표 Table 1. DPPH, ABTS Radicals scavenging assay and total polypphenol contents of A. lancifolium extracts 그림 Fig. 3. Effect of A. lancifolium leaf extracts on cell viability of RAW264.7 macrophages The cells were treated with various concentrations of leaf extracts for 24 h. Cell viability was assessed with MTT assay. Data are expressed as mean±standard deviation of triplicate experiments. Abbreviations: CTL, Control; LPS, lipopolysaccharide; AW, A. lancifolium water extract; AM, A. lancifolium methanol extract. 그림 Fig. 4. Effect of A. lancifolium leaf extracts on the NO production and iNOS protein levels in LPS-stimulated RAW264.7 cells RAW264.7 cells were pretreated with the indicated concentrations of leaf extracts for 1 h prior to LPS (100 ng/mL) for 24 h. The NO concentrations in medium were determined by Griess reagent assay (a). Whole cell lysates were then subjected to Western blot analysis(b). Data are expressed as mean±standard deviation of triplicate experiments. Abbreviations: iNOS, inducible nitric oxide synthase; LPS, lipopolysaccharide; AW, A. lancifolium water extract; AM, A. lancifolium methanol extract. 그림 Fig. 5. Inhibitory effect of A. lancifolium leaf extracts on the secretion levels of IL-6(a) and TNF-α(b) in LPS-stimulated RAW264.7 cells RAW264.7 cells were pretreated with the indicated concentrations of leaf extracts for 1 h prior to LPS (100 ng/mL) for 24 h. Cytokine secretion was determined in culture supernatant by ELISA. Data are expressed as mean±standard deviation of triplicate experiments. Abbreviations: CTL, Control; AW, A. lancifolium water extract; AM, A. lancifolium methanol extract. 그림 Fig. 6. Effect of A. lancifolium leaf extracts on the astringent activity Data are expressed as mean±standard deviation of triplicate experiments. Abbreviations: AA, L-ascorbic acid(10 mg/L); GA, Gallic acid(10 mg/mL); AW, A. lancifolium water extract; AM, A. lancifolium methanol extract.
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