[논문]고지방식이 유도 비만 마우스에서 아연 보충이 골격 근육의 아연 수송체 ZIP7 수준과 혈당 조절에 미치는 영향

주치앤징; 정자용

doi:10.4163/jnh.2021.54.6.594

고지방식이 유도 비만 마우스에서 아연 보충이 골격 근육의 아연 수송체 ZIP7 수준과 혈당 조절에 미치는 영향
Dietary zinc supplementation in high-fat diet-induced obese mice: Effects on the skeletal muscle ZIP7 expression and blood glucose regulation 원문보기

Journal of nutrition and health, v.54 no.6, 2021년, pp.594 - 602

주치앤징 (경희대학교 생활과학대학 식품영양학과) , 정자용 (경희대학교 생활과학대학 식품영양학과)

초록
AI-Helper

본 연구는 고지방식이로 유도한 비만 동물모델에서 아연의 식이를 통한 보충 급여가 혈당 조절과 골격 근육의 ZIP7의 작용에 미치는 영향에 대해 살펴보았다. 고지방식이를 공급한 HF군은 정상 대조군에 비하여 단위 체중당 골격 근육 무게가 유의하게 감소하였으며, 혈당은 유의적으로 증가하였다. 고지방식이와 함께 아연을 보충 공급한 HF+Zn군은 아연을 보충하지 않은 HF군과 비교하여, 공복 혈당과 경구 포도당 부하 후 혈당 증가 면적이 유의하게 감소하였다. 또한, HF+Zn군은 HF군에 비해 골격 근육의 ZIP7 단백질 수준이 유의하게 증가하였으며, AKT 활성과 GLUT4 단백질 수준도 유의하게 증가하는 것으로 나타났다. 이상의 결과를 종합해 볼 때, 아연 보충은 비만으로 인한 고혈당 증세를 완화하는 효과를 나타내며, 이는 골격 근육에서의 ZIP7 아연 수송체에 의한 당 대사 조절과 관련이 있을 것으로 생각된다.

Abstract ▼ AI-Helper

Purpose: The zinc transporter ZIP7 is known to regulate glucose metabolism in skeletal muscles, and skeletal muscles are known to play a critical role in glycemic control. The present study examines the effects of dietary zinc supplementation on the blood glucose concentration and expression of ZIP7 in skeletal muscle obtained from obese mice fed a high-fat diet (HF). Methods: C57BL/6J male mice were divided into three groups and were administered either a HF (60% of total calories from fat), HF supplemented with zinc (HF+Zn, 60% calories from fat + 300 mg zinc/kg diet), or low-fat diet (CON, 10% calories from fat), for 15 weeks. Results: Compared to CON group mice, the final body weights and adipose tissue weights were significantly increased, while the skeletal muscle weights were significantly decreased in mice belonging to the HF and HF+Zn groups. The HF+Zn group had significantly lower levels of fasting blood glucose concentrations than the HF group. Similarly, zinc supplementation significantly decreased the HF-elevated area under the curve values obtained from the oral glucose tolerance test. Skeletal muscle protein levels of ZIP7 in samples obtained from the HF group were significantly decreased as compared to the CON group. Conversely, the skeletal ZIP7 protein levels in the HF+Zn group were significantly increased as compared to the HF group. Moreover, the protein levels of phosphorylated-AKT and glucose transporter 4 in the skeletal muscle were significantly increased subsequent to zinc supplementation. Conclusion: Our data demonstrates that zinc supplementation up-regulates the skeletal muscle ZIP7 expression, which is associated with improved glucose tolerance in the obesity.

주제어

표/그림 (4)

그림 Fig. 1. Effects of dietary zinc supplementation on (A) total body weight, (B) adipose tissue weight, and (C) skeletal muscle weight in mice fed HF. One-way analysis of variance followed by Duncan's multiple range test was performed. Horizontal bar indicates mean values in each group. CON, low-fat diet (▵); HF, high-fat diet (▪); HF+Zn, high-fat diet and zinc (•) (n = 15/group). ¹⁾Indicates statistical significance (p < 0.05) vs. CON group.
그림 Fig. 2. Effects of dietary zinc supplementation on (A) blood glucose concentrations, (B) area under curve values upon oral glucose tolerance test in mice fed HF. Fasting blood glucose was measured after 12 hours of fasting. Post-prandial blood glucose was measured after 15, 30, 60, 90, and 120 minutes of oral feeding of glucose solution (2 g/kg) to the fasted mouse. Values are presented as means ± SEM. One-way analysis of variance followed by Duncan's multiple range test was performed. Different letters indicate statistical significance (p < 0.05). OGTT, oral glucose tolerance test; AUC, area under the curve; CON, low-fat diet; HF, high-fat diet; HF+Zn, high-fat diet and zinc.
그림 Fig. 3. Effects of dietary zinc supplementation on ZIP7 protein levels in mice fed HF. ZIP7 and β-actin protein expression in the skeletal muscle were determined by Western blot analyses. Representative images are presented. ZIP7 protein expression levels are quantified and normalized relative to β-actin protein level. The relative values are expressed as mean ± SEM. One-way analysis of variance followed by Duncan's multiple range test was performed. Different letters indicate statistical significance (p < 0.05). CON, low-fat diet; HF, high-fat diet; HF+Zn, high-fat diet and zinc.
그림 Fig. 4. Effects of dietary zinc supplementation on (A) pAKT and (B) GLUT4 protein levels in mice fed high-fat diet. pAKT, AKT, GLUT4 and β-actin protein expression in the skeletal muscle were determined by Western blot analyses. Representative images are presented. pAKT, AKT, and GLUT4 protein expression levels are quantified and normalized relative to β-actin protein level. The relative values are expressed as mean ± SEM. One-way analysis of variance followed by Duncan's multiple range test was performed. Different letters indicate statistical significance (p < 0.05). CON, low-fat diet; HF, high-fat diet; HF+Zn, high-fat diet and zinc; pAKT, phosphorylated-AKT; GLUT4, glucose transporter 4; NS, not significant.

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저자의 다른 논문 :

표제어: PCR

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