[논문]소시호탕(小柴胡湯)이 thioacetamide로 유발된 간섬유증 동물 모델에 미치는 영향

이세희; 오민혁; 신미래; 이지혜; 노성수

doi:10.22246/jikm.2021.42.6.1223

소시호탕(小柴胡湯)이 thioacetamide로 유발된 간섬유증 동물 모델에 미치는 영향
Effect of Sosiho-tang on a Thioacetamide-induced Liver Fibrosis Mouse Model 원문보기

대한한방내과학회지 = The journal of internal Korean medicine, v.42 no.6, 2021년, pp.1223 - 1236

이세희 (대구한의대학교 한의과대학 본초약리학교실) , 오민혁 (대구한의대학교 한의과대학 본초약리학교실) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 이지혜 (세명대학교 한의과대학 한의예과) , 노성수 (대구한의대학교 한의과대학 본초약리학교실)

Abstract ▼ AI-Helper

Objectives: The aim of the current study was to investigate the effect of Sosiho-tang on thioacetamide (TAA)-induced liver fibrosis in mice and to elucidate its underlying mechanisms. Methods: The mice were divided into 4 groups: Normal mice (Normal), TAA-induced control mice (Control), TAA-induced and silymarin-treated (50 mg/kg) mice (Silymarin), and TAA-induced and Sosiho-tang treated (200 mg/kg) mice (SSHT). Liver fibrosis was induced via intraperitoneal injection of TAA three times a week for 8 weeks. Silymarin and Sosiho-tang were concomitantly administered for 8 weeks. Serum and liver tissues were then collected and the anti-oxidant and inflammatory protein levels in the liver tissues were evaluated using western blotting. Results: SSHT administration significantly reduced the levels of AST, ALT, ammonia, and MPO in the serum. SSHT also significantly down-regulated liver NADPH oxidase and regulated the Nrf2/Keap1 signaling pathway. SSHT treatment downregulated the liver NF-κB levels and suppressed inflammatory cytokines. SSHT treatment also decreased bile acid-related factors, such as CYP7A1 and NTCP, and fibrosis-related factors, such as α-SMA and Collagen I. Conclusions: Taken together, these data suggest that SSHT administration suppressed the progression of liver fibrosis by activating the Nrf2/Keap1 pathway and inhibiting NF-κB.

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표/그림 (9)

표 Table 1. Composition of Sosiho-tang
그림 Fig. 1. Levels of AST, ALT, ammonia, and MPO in serum. Serum AST level : A, Serum ALT level : B, Serum ammonia concentration : C, Serum MPO concentration : D. Normal : normal mice, Control : TAA-induced control mice, Silymarin : TAA-induced with silymarin 50 mg/kg body weight mice, SSHT : TAA-induced with Sosiho-tang water extract 200 mg/kg body weight mice. All data are expressed as means±SD (n=9). Significance : ^###p<0.001 vs. Normal mice, **p<0.01, ***p<0.001 vs. Control mice.
그림 Fig. 2. Expressions of NADPH oxidase in liver tissue. Normal : normal mice, Control : TAA-induced control mice, Silymarin : TAA-induced with silymarin 50 mg/kg body weight mice, SSHT : TAA-induced with Sosiho-tang water extract 200 mg/kg body weigh mice. All data are expressed as means±SD (n=9). Significance : ^#p<0.05, ^###p<0.001 vs. Normal mice, *p<0.05, **p<0.01, and ***p<0.001 vs. Control mice.
그림 Fig. 3. Expressions of antioxidant proteins in liver tissue. Normal : normal mice, Control : TAA-induced control mice, Silymarin : TAA-induced with silymarin 50 mg/kg body weight mice, SSHT : TAA-induced with Sosiho-tang water extract 200 mg/kg body weight mice. All data are expressed as means±SD(n=9). Significance : ^#p<0.05, _##p<0.01, and _###p<0.01 vs. Normal mice, *p<0.05, **p<0.01 vs. Control mice.
그림 Fig. 4. Expressions of inflammatory proteins in liver tissue. Normal : normal mice, Control : TAA-induced control mice, Silymarin : TAA-induced with silymarin 50 mg/kg body weight mice, SSHT : TAA-induced with Sosiho-tang water extract 200 mg/kg body weight mice. All data are expressed as means±SD (n=9). Significance : ^##p<0.01, ^###p<0.001 vs. Normal mice, *p<0.05, **p<0.01, and ***p<0.001 vs. Control mice.
그림 Fig. 5. Expressions of inflammatory cytokines in liver tissue. Normal : normal mice, Control : TAA-induced control mice, Silymarin : TAA-induced with silymarin 50 mg/kg body weight mice, SSHT : TAA-induced with Sosiho-tang water extract 200 mg/kg body weight mice. All data are expressed as means±SD (n=9). Significance : ^###p<0.001 vs. Normal mice, *p<0.05, **p<0.01, and ***p<0.001 vs. Control mice.
그림 Fig. 6. Expressions of CYP7A1 and NTCP in liver tissue. Normal : normal mice, Control : TAA-induced control mice, Silymarin : TAA-induced with silymarin 50 mg/kg body weight mice, SSHT : TAA-induced with Sosiho-tang water extract 200 mg/kg body weight mice. All data are expressed as means±SD (n=9). Significance : ^##p<0.01, ^###p<0.001 vs. Normal mice, *p<0.05, **p<0.01 vs. Control mice.
그림 Fig. 7. Expressions of α-SMA and Collagen Ⅰ in liver tissue. Fig. 7. Expressions of α-SMA and Collagen Ⅰ in liver tissue. Normal : normal mice, Control : TAA-induced control mice, Silymarin : TAA-induced with silymarin 50 mg/kg body weight mice, SSHT : TAA-induced with Sosiho-tang water extract 200 mg/kg body weight mice. All data are expressed as means±SD (n=9). Significance : ^##p<0.01, ^###p<0.001 vs. Normal mice, *p<0.05, **p<0.01 vs. Control mice.
그림 Fig. 8. Histopathological analysis. Masson's trichrome (MT) staining. (Original magnification×200, magnification bar=100 μm). Normal : normal mice, Control : TAA-induced control mice, Silymarin : TAA-induced with silymarin 50 mg/kg body weight/day mice, SSHT : TAA-induced with Sosiho-tang water extract 200 mg/kg body weight/day mice.

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