[논문]Thioacetamide로 유도된 간섬유화 모델에서 생간건비탕(生肝健脾湯)의 보호 효과

최정원; 정성미; 신미래; 정다운; 노성수

doi:10.6116/kjh.2022.37.6.9.

Thioacetamide로 유도된 간섬유화 모델에서 생간건비탕(生肝健脾湯)의 보호 효과
Protective effect of Saenggangeonbi-tang on liver fibrosis induced by thioacetamide 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.6, 2022년, pp.9 - 17

최정원 (대구한의대학교 한의과대학 본초약리학교실) , 정성미 (정성미한의원) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 정다운 (대구한의대학교 간질환한약융복합활용연구센터) , 노성수 (대구한의대학교 한의과대학 본초약리학교실)

Abstract ▼ AI-Helper

Objective : In modern society, liver diseases such as liver fibrosis are on the rise as inflammation and wound healing processes of the liver are repeated due to factors such as drinking, smoking, and stress. This study was conducted to evaluate the effect of Saenggangeonbi-tang (SGGBT) on thioacetamide (TAA)-induced liver fibrosis. Methods : The mice were divided into 4 groups for examination (n=6): Normal group (Nor), distilled water-treated liver fibrosis mice (Con), silymarin 50 mg/kg-treated liver fibrosis mice (Sily), SGGBT 200 mg/kg-treated liver fibrosis mice (S200). Liver fibrosis was established in the mice via TAA for 8 weeks (1 week 100 mg/kg, 2,3 weeks 200 mg/kg, 4-8 weeks 400 mg/kg, three times a week, intraperitoneal injection) and they were administered silymarin and SGGBT (every day, oral administration) with the TAA. Results : SGGBT significantly decreased the levels of aspartate aminotransferase, alanine aminotransferanse, ammonia, and myeloperoxidase in serum increased by liver fibrosis. As a result of confirming H&E and MT staining, it was confirmed that SGGBT reduced damage and inflammatory cell infiltration in liver tissue, and alleviated changes in collagen fiber deposition and histological fibrosis. Also, it was confirmed through PAS staining that it reduced glycogen deposition in liver tissue. In addition, SGGBT significantly decreased the NADPH oxidases as well as significantly modulated the expression of MMP-2 and TIMP-2. Conclusions : These results suggest that SGGBT regulates the expression of MMP/TIMP protein through inhibition of oxidative stress and alleviates liver fibrosis by reducing collagen and glycogen deposition in liver tissue.

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표/그림 (9)

표 Table 1. Contents of Saenggangeonbi-tang
표 Table 2. Total Polyphenol and Total Flavonoid Contents of Saenggangeonbi-tang
표 Table 3. DPPH and ABTS Radical Scavenging Activity of Saenggangeonbi-tang
그림 Fig. 1. Liver histological examination through H&E staining. Hematoxylin and eosin; H&E (Original magnification ×200, scale bar; 100 μm). Normal mice; Nor, liver fibrosis mice; Con, silymarin 50 mg/kg body weight/day-treated liver fibrosis mice; Sily, Saenggangeonbi-tang 200 mg/kg body weight/day-treated liver fibrosis mice; S200.
그림 Fig. 2. Liver histological examination through MT staining. Masson's trichrome; MT (Original magnification ×200, scale bar; 100 μm). Normal mice; Nor, liver fibrosis mice; Con, silymarin 50 mg/kg body weight/day-treated liver fibrosis mice; Sily, Saenggangeonbi-tang 200 mg/kg body weight/day-treated liver fibrosis mice; S200.
표 Table 2. Body Weight and Food Efficiency Ratio
그림 Fig. 3. Liver histological examination through PAS staining. Periodic acid-Schiff; PAS (Original magnification ×200, scale bar; 100 μm). Normal mice; Nor, liver fibrosis mice; Con, silymarin 50 mg/kg body weight/day-treated liver fibrosis mice; Sily, Saenggangeonbi-tang 200 mg/kg body weight/day-treated liver fibrosis mice; S200.
그림 Fig. 4. Expression of NADPH oxidase in liver. Normal mice; Nor, liver fibrosis mice; Con, silymarin 50 mg/kg body weight/day-treated liver fibrosis mice; Sily, Saenggangeonbi-tang 200 mg/kg body weight/day-treated liver fibrosis mice; S200. All data are expressed as mean ± SD (n=6). Significance: ^#p < 0.05, ^###p < 0.001 versus Nor group, *p < 0.05, **p < 0.01, ***p < 0.001 versus Con group.
그림 Fig. 5. Expression of MMP-2 and TIMP-2 in liver. Normal mice; Nor, liver fibrosis mice; Con, silymarin 50 mg/kg body weight/day-treated liver fibrosis mice; Sily, Saenggangeonbi-tang 200 mg/kg body weight/day-treated liver fibrosis mice; S200. All data are expressed as mean ± SD (n=6). Significance: ^#p<0.05, ^##p < 0.01, versus Nor group, *p < 0.05, **p < 0.01, ***p < 0.001 versus Con group.

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