[논문]Microsatellite marker를 이용하여 송이감염묘로부터 발생한 송이 확인

가강현; 김희수; 이제완; 장영선; 유림

doi:10.4489/kjm.20210045

[국내논문] Microsatellite marker를 이용하여 송이감염묘로부터 발생한 송이 확인
Confirmation of Fruiting Bodies of Tricholoma matsutake Occurring from Matsutake-infected Pine Trees Using Microsatellite Markers 원문보기

한국균학회지 = The Korean journal of mycology, v.49 no.4, 2021년, pp.469 - 476

가강현 (국립산림과학원 산림생명자원연구부) , 김희수 (산림농원) , 이제완 (국립산림과학원 산림생명자원연구부) , 장영선 (국립산림과학원 산림생명자원연구부) , 유림 (국립산림과학원 산림생명자원연구부)

초록
AI-Helper

송이는 상업적으로 중요한 야생 식용 버섯이다. 현재까지 송이 재배는 송이감염묘를 이용한 것이 유일한 방법이다. 2020년 송이감염묘로부터 21개의 송이가 홍천 송이시험지에서 발생하였다. 송이감염묘 생산지역의 송이와 이식한 송이감염묘로부터 발생한 송이는 모니터링을 통해 확인하였고, 13개의 microsatellite 마커를 이용하여 분석한 결과 동일한 것이 확인되었다. 송이감염묘는 송이균환 형성과 송이 발생에 성공하였고, 송이발생은 2010년과 2017년부터 2020년까지 발생하였다. 결론적으로 송이 버섯발생은 송이감염묘의 송이균에서 기원한 것이다.

Abstract ▼ AI-Helper

Tricholoma matsutake is commercially important wild edible mushroom. The only method for cultivation of T. matsutake has been to use matsutake-infected pine trees. In this study, twenty-one fruiting bodies were collected from the matsutake-infected pine trees in the Hongcheon experimental site in 2020. The fruiting bodies from the existing production area of matsutake-infected pine tree and from its transplantation site were found to be identical through monitoring and analysis of 13 microsatellite markers. Transplanted matsutake-infected pine trees succeeded in fairy-ring formation and matsutake fruiting in 2010 and 2017 to 2020. In conclusion, the matsutake mycelium of transplanted matsutake-infected pine tree originates from the existing matsutake-infected pine tree production.

Keyword

표/그림 (8)

그림 Fig. 1. Twenty-one fruiting bodies of Tricholoma matustake occurred from transplanted matsutakeinfected pine trees in Hongcheon experiment site.
표 Table 1. Analysis of fruiting bodies of Tricholoma matsutake using microsatellite markers to distinguish between production site of matsutake-infected pine trees and transplanted site of matsutake-infected pine trees.
표 Table 2. Microsatellite markers used in this study.
그림 Fig. 2. Principal coordinates analysis according to genetic distance between the fruiting body of existing production area and the fruiting body of transplanted area of matsutake-infected pine trees. The fruiting bodies with the same genotype are located at the same point. Number indicates the number of tested fruiting body used in this study (Table 1).
그림 Fig. 3. Transplanted positions of matsutake-infected pine trees (square) made in Hongcheon matsutake pine forest and fruiting bodies (circle) occurred from them in 2020.
그림 Fig. 4. Transplanted positions of matsutake-infected pine trees (square) made in Hongcheon matsutake pine forest and fruiting bodies (circle) occurred from them in 2020.
그림 Fig. 5. Transplanted positions of matsutake-infected pine trees (square) made in Yangyang matsutake pine forest and fruiting bodies (circle) occurred from them in 2020.
표 Table 3. Genetic distances between the fruiting body of existing production area and the fruiting body of transplanted area of matsutake-infected pine trees based on Codom-Genotypic distance.

참고문헌 (14)

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