[논문]청피 물 추출물의 항염증 효과와 기전 연구

안태석; 황덕상; 이진무; 장준복; 이창훈

doi:10.15204/jkobgy.2021.34.1.034

청피 물 추출물의 항염증 효과와 기전 연구
Anti-inflammatory Effect and Mechanism of Citri Reticulatae Viride Pericarpium Water Extract 원문보기

大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, v.34 no.1, 2021년, pp.34 - 47

안태석 (경희대학교 대학원 임상한의학과) , 황덕상 (경희대학교 대학원 임상한의학과) , 이진무 (경희대학교 대학원 임상한의학과) , 장준복 (경희대학교 대학원 임상한의학과) , 이창훈 (경희대학교 대학원 임상한의학과)

Abstract ▼ AI-Helper

Objectives: This study was designed to examine anti-inflammatory effect and mechanism of Citri Reticulatae Viride Pericarpium water extract (CRE). Methods: Cell cytotoxicity was tested with RAW 264.7 cells. To investigate anti-inflammatory effect of CRE in lipopolysaccharide (LPS)-induced RAW 264.7 cell, we measured nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10). In addition, mitrogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) were examined by western blotting in LPS-induced RAW 264.7 cell with treated CRE. Results: In cytotoxicity analysis, CRE does not affect cell cytotoxicity. As compared with the control group, the expression of NO, PGE2, TNF-α, IL-6 were significantly decreased, and IL-10 was significantly increased in LPS-induced RAW 264.7 cell with treated CRE. As a result of Western blotting, there was concentration-dependent inhibition of pp38, pERK in MAPK pathway and significant reduction of pp65 in the NF-κB pathway. Conclusions: CRE might have anti-inflammatory effect in LPS-induced macrophages by promoting the production of IL-10.

주제어

표/그림 (8)

그림 Fig. 1. Cytotoxicity of CRE on RAW 264.7 cell. *CRE : Citri Reticulatae Viride Pericarpium water extract
그림 Fig. 2. Production of NO from macrophage stimulated by CRE and LPS (**p<0.01). *CRE : Citri Reticulatae Viride Pericarpium water extract, ^†LPS : lipopolysaccharide, ^‡NO : nitric oxide
그림 Fig. 3. Production of PGE2 from macrophage stimulated by CRE and LPS (*p<0.05). *CRE : Citri Reticulatae Viride Pericarpium water extract, ^†LPS : lipopolysaccharide, ^‡PGE2 : prostaglandin E2
그림 Fig. 4. Production of TNF-α from macrophage stimulated by CRE and LPS (**p<0.01). *CRE : Citri Reticulatae Viride Pericarpium water extract, ^†LPS : lipopolysaccharide, ^‡TNF-α : tumor necrosis factor-alpha
그림 Fig. 5. Production of IL-6 from macrophage stimulated by CRE and LPS (**p<0.01). *CRE : Citri Reticulatae Viride Pericarpium water extract, ^†LPS : lipopolysaccharide, ^‡IL-6 : interleukin-6
그림 Fig. 6. Production of IL-10 from macrophage stimulated by CRE and LPS (**p<0.01). *CRE : Citri Reticulatae Viride Pericarpium water extract, ^†LPS : lipopolysaccharide, ^‡IL-10 : interleukin-10
그림 Fig. 7. Inhibition of MAPK pathway activation by CRE and LPS on RAW 264.7 cell. *CRE : Citri Reticulatae Viride Pericarpium water extract, ^†LPS : lipopolysaccharide
그림 Fig. 8. Inhibition of NF-κB pathway activation by CRE and LPS on RAW 264.7 cell. *CRE : Citri Reticulatae Viride Pericarpium water extract, ^†LPS : lipopolysaccharide

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저자의 다른 논문 :

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